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Bacterial toxicity/compatibility of platinum nanospheres, nanocuboids and nanoflowers.

Gopal J, Hasan N, Manikandan M, Wu HF - Sci Rep (2013)

Bottom Line: The bacterio-toxic or compatible properties of these five different sized Pt NPs with the clinical pathogen, Pseudomonas aeruginosa were explored by many analytical methods such as the conventional plate count method, matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS), fluorescence microscopy and fluorescence sensoring techniques.The results revealed that the 1-3 nm sized (P1 and P2) Pt NPs showed bacterio-toxic properties while the 4-21 nm (P3, P4 and P5) Pt NPs exhibited bacterio-compatible properties.The information released from this study is significantly important to future clinical, medical, biological and biomedical applications of Pt NPs.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 70 Lien-Hai Road, 80424, Taiwan.

ABSTRACT
For the first time, we have investigated the bacterial toxicity or compatibility properties of Pt nanoparticles (NPs) with different sizes (P1, P2, P3, P4 and P5). The bacterio-toxic or compatible properties of these five different sized Pt NPs with the clinical pathogen, Pseudomonas aeruginosa were explored by many analytical methods such as the conventional plate count method, matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS), fluorescence microscopy and fluorescence sensoring techniques. The results revealed that the 1-3 nm sized (P1 and P2) Pt NPs showed bacterio-toxic properties while the 4-21 nm (P3, P4 and P5) Pt NPs exhibited bacterio-compatible properties. This is the first study which reports the bacterial toxicity of Pt NPs. The information released from this study is significantly important to future clinical, medical, biological and biomedical applications of Pt NPs.

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Related in: MedlinePlus

(a), Fluorescence spectra of the AO stained P.aeruginosa control cells and cells incubated with 8.5 mg/L concentrations of P1, P2, P3, P4 and P5 Pt NPs for 6 h. (b), Fluorescence spectra of the AO stained P.aeruginosa control cells and cells incubated with 15 mg/L concentrations of P1, P2, P3, P4 and P5 Pt NPs for 6 h.
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f5: (a), Fluorescence spectra of the AO stained P.aeruginosa control cells and cells incubated with 8.5 mg/L concentrations of P1, P2, P3, P4 and P5 Pt NPs for 6 h. (b), Fluorescence spectra of the AO stained P.aeruginosa control cells and cells incubated with 15 mg/L concentrations of P1, P2, P3, P4 and P5 Pt NPs for 6 h.

Mentions: Finally, we also confirm the size dependant bacteriotoxic/compatible property of the Pt NPs using fluorescence spectroscopy. Recently, we have reported the use of acridine orange based techniques for differentiation of live/dead cells based on the 547 nm peak during fluorescence spectroscopic studies (unpublished data). Since the dead cells take up more green fluorescence, the intensity of this peak was higher in cell mixtures where dead cells dominated. We also used this technique to assay the cell viability of the P.aeruginosa cells after incubation with the different Pt NPs. As observed from Fig. 5a, the P1 and P2 Pt NPs showed highest 547 nm peaks compared to all the others at NPs concentration of 8.5 mg/L and also at 15 mg/L (Fig. 5b). The P3, P4, P5 and control showed lower intensity confirming their bacterio-compatibility. The same trend was observed after 9 h incubation (Fig. S5A) and even up to 24 h incubation (Fig. S5B) too. These results further confirmed the bacteriotoxic property exhibited by the P1 and P2 Pt NPs and the bacterio-compatible property exhibited by the P3, P4 and P5 Pt NPs.


Bacterial toxicity/compatibility of platinum nanospheres, nanocuboids and nanoflowers.

Gopal J, Hasan N, Manikandan M, Wu HF - Sci Rep (2013)

(a), Fluorescence spectra of the AO stained P.aeruginosa control cells and cells incubated with 8.5 mg/L concentrations of P1, P2, P3, P4 and P5 Pt NPs for 6 h. (b), Fluorescence spectra of the AO stained P.aeruginosa control cells and cells incubated with 15 mg/L concentrations of P1, P2, P3, P4 and P5 Pt NPs for 6 h.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3569627&req=5

f5: (a), Fluorescence spectra of the AO stained P.aeruginosa control cells and cells incubated with 8.5 mg/L concentrations of P1, P2, P3, P4 and P5 Pt NPs for 6 h. (b), Fluorescence spectra of the AO stained P.aeruginosa control cells and cells incubated with 15 mg/L concentrations of P1, P2, P3, P4 and P5 Pt NPs for 6 h.
Mentions: Finally, we also confirm the size dependant bacteriotoxic/compatible property of the Pt NPs using fluorescence spectroscopy. Recently, we have reported the use of acridine orange based techniques for differentiation of live/dead cells based on the 547 nm peak during fluorescence spectroscopic studies (unpublished data). Since the dead cells take up more green fluorescence, the intensity of this peak was higher in cell mixtures where dead cells dominated. We also used this technique to assay the cell viability of the P.aeruginosa cells after incubation with the different Pt NPs. As observed from Fig. 5a, the P1 and P2 Pt NPs showed highest 547 nm peaks compared to all the others at NPs concentration of 8.5 mg/L and also at 15 mg/L (Fig. 5b). The P3, P4, P5 and control showed lower intensity confirming their bacterio-compatibility. The same trend was observed after 9 h incubation (Fig. S5A) and even up to 24 h incubation (Fig. S5B) too. These results further confirmed the bacteriotoxic property exhibited by the P1 and P2 Pt NPs and the bacterio-compatible property exhibited by the P3, P4 and P5 Pt NPs.

Bottom Line: The bacterio-toxic or compatible properties of these five different sized Pt NPs with the clinical pathogen, Pseudomonas aeruginosa were explored by many analytical methods such as the conventional plate count method, matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS), fluorescence microscopy and fluorescence sensoring techniques.The results revealed that the 1-3 nm sized (P1 and P2) Pt NPs showed bacterio-toxic properties while the 4-21 nm (P3, P4 and P5) Pt NPs exhibited bacterio-compatible properties.The information released from this study is significantly important to future clinical, medical, biological and biomedical applications of Pt NPs.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 70 Lien-Hai Road, 80424, Taiwan.

ABSTRACT
For the first time, we have investigated the bacterial toxicity or compatibility properties of Pt nanoparticles (NPs) with different sizes (P1, P2, P3, P4 and P5). The bacterio-toxic or compatible properties of these five different sized Pt NPs with the clinical pathogen, Pseudomonas aeruginosa were explored by many analytical methods such as the conventional plate count method, matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS), fluorescence microscopy and fluorescence sensoring techniques. The results revealed that the 1-3 nm sized (P1 and P2) Pt NPs showed bacterio-toxic properties while the 4-21 nm (P3, P4 and P5) Pt NPs exhibited bacterio-compatible properties. This is the first study which reports the bacterial toxicity of Pt NPs. The information released from this study is significantly important to future clinical, medical, biological and biomedical applications of Pt NPs.

Show MeSH
Related in: MedlinePlus