Limits...
Bacterial toxicity/compatibility of platinum nanospheres, nanocuboids and nanoflowers.

Gopal J, Hasan N, Manikandan M, Wu HF - Sci Rep (2013)

Bottom Line: The bacterio-toxic or compatible properties of these five different sized Pt NPs with the clinical pathogen, Pseudomonas aeruginosa were explored by many analytical methods such as the conventional plate count method, matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS), fluorescence microscopy and fluorescence sensoring techniques.The results revealed that the 1-3 nm sized (P1 and P2) Pt NPs showed bacterio-toxic properties while the 4-21 nm (P3, P4 and P5) Pt NPs exhibited bacterio-compatible properties.The information released from this study is significantly important to future clinical, medical, biological and biomedical applications of Pt NPs.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 70 Lien-Hai Road, 80424, Taiwan.

ABSTRACT
For the first time, we have investigated the bacterial toxicity or compatibility properties of Pt nanoparticles (NPs) with different sizes (P1, P2, P3, P4 and P5). The bacterio-toxic or compatible properties of these five different sized Pt NPs with the clinical pathogen, Pseudomonas aeruginosa were explored by many analytical methods such as the conventional plate count method, matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS), fluorescence microscopy and fluorescence sensoring techniques. The results revealed that the 1-3 nm sized (P1 and P2) Pt NPs showed bacterio-toxic properties while the 4-21 nm (P3, P4 and P5) Pt NPs exhibited bacterio-compatible properties. This is the first study which reports the bacterial toxicity of Pt NPs. The information released from this study is significantly important to future clinical, medical, biological and biomedical applications of Pt NPs.

Show MeSH

Related in: MedlinePlus

MALDI-MS spectra of P. aeruginosa incubated with (a) no Pt NPs (control) and 15 mg/L concentrations of (b) P1 , (c) P2 , (d) P3, (e) P4, (f) P5 Pt NPs for 24 h, showing distinctly lesser peaks in P1 and P2.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3569627&req=5

f3: MALDI-MS spectra of P. aeruginosa incubated with (a) no Pt NPs (control) and 15 mg/L concentrations of (b) P1 , (c) P2 , (d) P3, (e) P4, (f) P5 Pt NPs for 24 h, showing distinctly lesser peaks in P1 and P2.

Mentions: MALDI-MS studies of the cells after incubation with Pt NPs (15 mg/L) revealed that compared to the control (Fig.S4A(a)), P1(Fig. S4A(b)) and P2 (Fig. S4A(c)) produced lower number of bacterial peaks. Similar to the plate count results, the P3, P4 and P5 Pt NP interaction lead to enhanced bacterial signals compared to the control at incubation periods of 3 h (Fig. S4A(d–f)), 6 h (Fig.S4B(d–f)) and 24 h (Fig. 3(d–f). P1 and P2 did not show any distinct difference in the results up to 3 h (Fig. S4A(b–c)), but beyond 6 h (Fig. 4(b–c)) even up to 24 h (Fig. 3(b–c)), distinct suppression on the bacterial signals were observed. Such suppression of bacterial peaks, following incubation with NPs has been reported earlier by our group4344. Thus, the MALDI-MS results indicate that the P1 and P2 Pt NPs showed bacterial toxicity, while the P3, P4 and P5 NPs showed bacterial compatibility.


Bacterial toxicity/compatibility of platinum nanospheres, nanocuboids and nanoflowers.

Gopal J, Hasan N, Manikandan M, Wu HF - Sci Rep (2013)

MALDI-MS spectra of P. aeruginosa incubated with (a) no Pt NPs (control) and 15 mg/L concentrations of (b) P1 , (c) P2 , (d) P3, (e) P4, (f) P5 Pt NPs for 24 h, showing distinctly lesser peaks in P1 and P2.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3569627&req=5

f3: MALDI-MS spectra of P. aeruginosa incubated with (a) no Pt NPs (control) and 15 mg/L concentrations of (b) P1 , (c) P2 , (d) P3, (e) P4, (f) P5 Pt NPs for 24 h, showing distinctly lesser peaks in P1 and P2.
Mentions: MALDI-MS studies of the cells after incubation with Pt NPs (15 mg/L) revealed that compared to the control (Fig.S4A(a)), P1(Fig. S4A(b)) and P2 (Fig. S4A(c)) produced lower number of bacterial peaks. Similar to the plate count results, the P3, P4 and P5 Pt NP interaction lead to enhanced bacterial signals compared to the control at incubation periods of 3 h (Fig. S4A(d–f)), 6 h (Fig.S4B(d–f)) and 24 h (Fig. 3(d–f). P1 and P2 did not show any distinct difference in the results up to 3 h (Fig. S4A(b–c)), but beyond 6 h (Fig. 4(b–c)) even up to 24 h (Fig. 3(b–c)), distinct suppression on the bacterial signals were observed. Such suppression of bacterial peaks, following incubation with NPs has been reported earlier by our group4344. Thus, the MALDI-MS results indicate that the P1 and P2 Pt NPs showed bacterial toxicity, while the P3, P4 and P5 NPs showed bacterial compatibility.

Bottom Line: The bacterio-toxic or compatible properties of these five different sized Pt NPs with the clinical pathogen, Pseudomonas aeruginosa were explored by many analytical methods such as the conventional plate count method, matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS), fluorescence microscopy and fluorescence sensoring techniques.The results revealed that the 1-3 nm sized (P1 and P2) Pt NPs showed bacterio-toxic properties while the 4-21 nm (P3, P4 and P5) Pt NPs exhibited bacterio-compatible properties.The information released from this study is significantly important to future clinical, medical, biological and biomedical applications of Pt NPs.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 70 Lien-Hai Road, 80424, Taiwan.

ABSTRACT
For the first time, we have investigated the bacterial toxicity or compatibility properties of Pt nanoparticles (NPs) with different sizes (P1, P2, P3, P4 and P5). The bacterio-toxic or compatible properties of these five different sized Pt NPs with the clinical pathogen, Pseudomonas aeruginosa were explored by many analytical methods such as the conventional plate count method, matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS), fluorescence microscopy and fluorescence sensoring techniques. The results revealed that the 1-3 nm sized (P1 and P2) Pt NPs showed bacterio-toxic properties while the 4-21 nm (P3, P4 and P5) Pt NPs exhibited bacterio-compatible properties. This is the first study which reports the bacterial toxicity of Pt NPs. The information released from this study is significantly important to future clinical, medical, biological and biomedical applications of Pt NPs.

Show MeSH
Related in: MedlinePlus