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Bacterial toxicity/compatibility of platinum nanospheres, nanocuboids and nanoflowers.

Gopal J, Hasan N, Manikandan M, Wu HF - Sci Rep (2013)

Bottom Line: The bacterio-toxic or compatible properties of these five different sized Pt NPs with the clinical pathogen, Pseudomonas aeruginosa were explored by many analytical methods such as the conventional plate count method, matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS), fluorescence microscopy and fluorescence sensoring techniques.The results revealed that the 1-3 nm sized (P1 and P2) Pt NPs showed bacterio-toxic properties while the 4-21 nm (P3, P4 and P5) Pt NPs exhibited bacterio-compatible properties.The information released from this study is significantly important to future clinical, medical, biological and biomedical applications of Pt NPs.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 70 Lien-Hai Road, 80424, Taiwan.

ABSTRACT
For the first time, we have investigated the bacterial toxicity or compatibility properties of Pt nanoparticles (NPs) with different sizes (P1, P2, P3, P4 and P5). The bacterio-toxic or compatible properties of these five different sized Pt NPs with the clinical pathogen, Pseudomonas aeruginosa were explored by many analytical methods such as the conventional plate count method, matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS), fluorescence microscopy and fluorescence sensoring techniques. The results revealed that the 1-3 nm sized (P1 and P2) Pt NPs showed bacterio-toxic properties while the 4-21 nm (P3, P4 and P5) Pt NPs exhibited bacterio-compatible properties. This is the first study which reports the bacterial toxicity of Pt NPs. The information released from this study is significantly important to future clinical, medical, biological and biomedical applications of Pt NPs.

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Related in: MedlinePlus

(a), Graph showing the growth curve of bacteria in the presence of 8.5 mg/L concentrations of P1, P2, P3, P4, P5 Pt NPs and control, based on the total viable counts obtained by plate count method. (b), Graph showing the growth curve of bacteria in the presence of 15 mg/L concentrations of P1, P2, P3, P4, P5 Pt NPs and control, based on the total viable counts obtained by plate count method.
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f2: (a), Graph showing the growth curve of bacteria in the presence of 8.5 mg/L concentrations of P1, P2, P3, P4, P5 Pt NPs and control, based on the total viable counts obtained by plate count method. (b), Graph showing the growth curve of bacteria in the presence of 15 mg/L concentrations of P1, P2, P3, P4, P5 Pt NPs and control, based on the total viable counts obtained by plate count method.

Mentions: Cells belonging to the clinical pathogen, P. aeruginosa were incubated with different sized Pt NPs P1, P2, P3, P4 and P5 for 3 h, 6 h, 9 h, 24 h and the bacterial counts following incubation with Pt NPs was determined by the plate count method. Fig. 2a and Fig. 2b display the growth curve of the bacterium in the presence of 8.5 mg/L (Fig. 2a) and 15 mg/L (Fig. 2b) concentrations of the various Pt NPs sizes. As can be observed from these figures, P1 and P2 showed reduction in the bacterial counts, especially after 6 h incubation, while all the other Pt NPs, (P3, P4 and P5) showed bacterial counts similar to or in some cases even more than the control. Even very high (almost saturated) concentrations of NPs (15 mg/L) did not demonstrate bactericidal activity.


Bacterial toxicity/compatibility of platinum nanospheres, nanocuboids and nanoflowers.

Gopal J, Hasan N, Manikandan M, Wu HF - Sci Rep (2013)

(a), Graph showing the growth curve of bacteria in the presence of 8.5 mg/L concentrations of P1, P2, P3, P4, P5 Pt NPs and control, based on the total viable counts obtained by plate count method. (b), Graph showing the growth curve of bacteria in the presence of 15 mg/L concentrations of P1, P2, P3, P4, P5 Pt NPs and control, based on the total viable counts obtained by plate count method.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3569627&req=5

f2: (a), Graph showing the growth curve of bacteria in the presence of 8.5 mg/L concentrations of P1, P2, P3, P4, P5 Pt NPs and control, based on the total viable counts obtained by plate count method. (b), Graph showing the growth curve of bacteria in the presence of 15 mg/L concentrations of P1, P2, P3, P4, P5 Pt NPs and control, based on the total viable counts obtained by plate count method.
Mentions: Cells belonging to the clinical pathogen, P. aeruginosa were incubated with different sized Pt NPs P1, P2, P3, P4 and P5 for 3 h, 6 h, 9 h, 24 h and the bacterial counts following incubation with Pt NPs was determined by the plate count method. Fig. 2a and Fig. 2b display the growth curve of the bacterium in the presence of 8.5 mg/L (Fig. 2a) and 15 mg/L (Fig. 2b) concentrations of the various Pt NPs sizes. As can be observed from these figures, P1 and P2 showed reduction in the bacterial counts, especially after 6 h incubation, while all the other Pt NPs, (P3, P4 and P5) showed bacterial counts similar to or in some cases even more than the control. Even very high (almost saturated) concentrations of NPs (15 mg/L) did not demonstrate bactericidal activity.

Bottom Line: The bacterio-toxic or compatible properties of these five different sized Pt NPs with the clinical pathogen, Pseudomonas aeruginosa were explored by many analytical methods such as the conventional plate count method, matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS), fluorescence microscopy and fluorescence sensoring techniques.The results revealed that the 1-3 nm sized (P1 and P2) Pt NPs showed bacterio-toxic properties while the 4-21 nm (P3, P4 and P5) Pt NPs exhibited bacterio-compatible properties.The information released from this study is significantly important to future clinical, medical, biological and biomedical applications of Pt NPs.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 70 Lien-Hai Road, 80424, Taiwan.

ABSTRACT
For the first time, we have investigated the bacterial toxicity or compatibility properties of Pt nanoparticles (NPs) with different sizes (P1, P2, P3, P4 and P5). The bacterio-toxic or compatible properties of these five different sized Pt NPs with the clinical pathogen, Pseudomonas aeruginosa were explored by many analytical methods such as the conventional plate count method, matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS), fluorescence microscopy and fluorescence sensoring techniques. The results revealed that the 1-3 nm sized (P1 and P2) Pt NPs showed bacterio-toxic properties while the 4-21 nm (P3, P4 and P5) Pt NPs exhibited bacterio-compatible properties. This is the first study which reports the bacterial toxicity of Pt NPs. The information released from this study is significantly important to future clinical, medical, biological and biomedical applications of Pt NPs.

Show MeSH
Related in: MedlinePlus