Limits...
Genomic variations define divergence of water/wildlife-associated Campylobacter jejuni niche specialists from common clonal complexes.

Hepworth PJ, Ashelford KE, Hinds J, Gould KA, Witney AA, Williams NJ, Leatherbarrow H, French NP, Birtles RJ, Mendonca C, Dorrell N, Wren BW, Wigley P, Hall N, Winstanley C - Environ. Microbiol. (2011)

Bottom Line: Studies employing multilocus sequence typing have indicated that some clonal complexes are more commonly associated with particular sources.By genome sequencing one representative of this diverse group (C. jejuni 1336), and a representative of the bank-vole niche specialist ST-3704 (C. jejuni 414), we identified deletions of genomic regions normally carried by human food-chain-associated C. jejuni.We suggest that the genomic divergence observed constitutes evidence of adaptation leading to niche specialization.

View Article: PubMed Central - PubMed

Affiliation: Institute of Infection and Global Health Institute of Integrative Biology National Centre for Zoonosis Research, University of Liverpool, Liverpool L69 3GA, UK.

Show MeSH

Related in: MedlinePlus

Venn diagrams showing orthologues shared by strains by 1336 and 414 with (A) C. jejuni NCTC11168 only and (B) 12 C. jejuni strains.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3569610&req=5

fig03: Venn diagrams showing orthologues shared by strains by 1336 and 414 with (A) C. jejuni NCTC11168 only and (B) 12 C. jejuni strains.

Mentions: In order to further investigate the divergence between WW/ST-3704 isolates and common human-associated isolates, we shotgun sequenced the genomes of strains 1336 (ST-841; a representative of the WW group, chosen because CGH indicated it as one of the most divergent from NCTC11168) and 414 (ST-3704). A basic summary of the sequencing data is shown in Table S3. When compared with the genome of C. jejuni NCTC11168 and each other, the genomes of strains 1336 and 414 harboured 260 and 334 unique ORFs respectively (Fig. 3A). These numbers were further reduced to 133 and 182, respectively, when a comparison was extended to include 12 genome sequenced C. jejuni strains (Fig. 3B).


Genomic variations define divergence of water/wildlife-associated Campylobacter jejuni niche specialists from common clonal complexes.

Hepworth PJ, Ashelford KE, Hinds J, Gould KA, Witney AA, Williams NJ, Leatherbarrow H, French NP, Birtles RJ, Mendonca C, Dorrell N, Wren BW, Wigley P, Hall N, Winstanley C - Environ. Microbiol. (2011)

Venn diagrams showing orthologues shared by strains by 1336 and 414 with (A) C. jejuni NCTC11168 only and (B) 12 C. jejuni strains.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3569610&req=5

fig03: Venn diagrams showing orthologues shared by strains by 1336 and 414 with (A) C. jejuni NCTC11168 only and (B) 12 C. jejuni strains.
Mentions: In order to further investigate the divergence between WW/ST-3704 isolates and common human-associated isolates, we shotgun sequenced the genomes of strains 1336 (ST-841; a representative of the WW group, chosen because CGH indicated it as one of the most divergent from NCTC11168) and 414 (ST-3704). A basic summary of the sequencing data is shown in Table S3. When compared with the genome of C. jejuni NCTC11168 and each other, the genomes of strains 1336 and 414 harboured 260 and 334 unique ORFs respectively (Fig. 3A). These numbers were further reduced to 133 and 182, respectively, when a comparison was extended to include 12 genome sequenced C. jejuni strains (Fig. 3B).

Bottom Line: Studies employing multilocus sequence typing have indicated that some clonal complexes are more commonly associated with particular sources.By genome sequencing one representative of this diverse group (C. jejuni 1336), and a representative of the bank-vole niche specialist ST-3704 (C. jejuni 414), we identified deletions of genomic regions normally carried by human food-chain-associated C. jejuni.We suggest that the genomic divergence observed constitutes evidence of adaptation leading to niche specialization.

View Article: PubMed Central - PubMed

Affiliation: Institute of Infection and Global Health Institute of Integrative Biology National Centre for Zoonosis Research, University of Liverpool, Liverpool L69 3GA, UK.

Show MeSH
Related in: MedlinePlus