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Isolation and Identification of hydrocarbon degrading bacteria from Ennore creek.

Subathra MK, Immanuel G, Suresh AH - Bioinformation (2013)

Bottom Line: Bioremediation is a non-invasive and cost effective technique for the clean-up of these petroleum hydrocarbons.Of the 113 crude oil degrading isolates 15 isolates were selected and cultivated in BH media with 1% crude oil as a sole carbon and energy source. 3 efficient crude oil bacterial isolates Bacillus subtilis I1, Pseudomonas aeruginosa I5 and Pseudomonas putida I8 were identified both biochemically and phylogenetically.The quantitative analysis of biodegradation is carried out gravimetrically and highest degradation rate, 55% was recorded by Pseudomonas aeruginosa I5 isolate.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, SRM Arts & Science College, Kattangulathur - 603203, Kancheepuram Dist, Tamilnadu, India.

ABSTRACT
The widespread problem caused due to petroleum products, is their discharge and accidental spillage in marine environment proving to be hazardous to the surroundings as well as life forms. Thus remediation of these hydrocarbons by natural decontamination process is of utmost importance. Bioremediation is a non-invasive and cost effective technique for the clean-up of these petroleum hydrocarbons. In this study we have investigated the ability of microorganisms present in the sediment sample to degrade these hydrocarbons, crude oil in particular, so that contaminated soils and water can be treated using microbes. Sediments samples were collected once in a month for a period of twelve months from area surrounding Ennore creek and screened for hydrocarbon degrading bacteria. Of the 113 crude oil degrading isolates 15 isolates were selected and cultivated in BH media with 1% crude oil as a sole carbon and energy source. 3 efficient crude oil bacterial isolates Bacillus subtilis I1, Pseudomonas aeruginosa I5 and Pseudomonas putida I8 were identified both biochemically and phylogenetically. The quantitative analysis of biodegradation is carried out gravimetrically and highest degradation rate, 55% was recorded by Pseudomonas aeruginosa I5 isolate.

No MeSH data available.


Related in: MedlinePlus

Molecular phylogenetic ananlysis by maximum likelihood method
© Copyright Policy - open-access
Related In: Results  -  Collection


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Figure 6: Molecular phylogenetic ananlysis by maximum likelihood method

Mentions: The systematic phylogenetic tree (Figure 6) of Bacillus sp (I1),Pseudomonas sp (I5) and Pseudomonas sp (I8) and other 15 strainswith high homology was constructed. Evolutionary analyseswere conducted in MEGA5.1. The evolutionary history wasinferred using the Neighbor-Joining method .The optimal treewith the sum of branch length = 11.01557767 are shown (next tothe branches). The evolutionary distances were computed usingthe Maximum Composite Likelihood method and are in theunits of the number of transversional substitutions per site. Theanalysis involved 18 nucleotide sequences. All positionscontaining gaps and missing data were eliminated. There werea total of 462 positions in the final dataset.


Isolation and Identification of hydrocarbon degrading bacteria from Ennore creek.

Subathra MK, Immanuel G, Suresh AH - Bioinformation (2013)

Molecular phylogenetic ananlysis by maximum likelihood method
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3569603&req=5

Figure 6: Molecular phylogenetic ananlysis by maximum likelihood method
Mentions: The systematic phylogenetic tree (Figure 6) of Bacillus sp (I1),Pseudomonas sp (I5) and Pseudomonas sp (I8) and other 15 strainswith high homology was constructed. Evolutionary analyseswere conducted in MEGA5.1. The evolutionary history wasinferred using the Neighbor-Joining method .The optimal treewith the sum of branch length = 11.01557767 are shown (next tothe branches). The evolutionary distances were computed usingthe Maximum Composite Likelihood method and are in theunits of the number of transversional substitutions per site. Theanalysis involved 18 nucleotide sequences. All positionscontaining gaps and missing data were eliminated. There werea total of 462 positions in the final dataset.

Bottom Line: Bioremediation is a non-invasive and cost effective technique for the clean-up of these petroleum hydrocarbons.Of the 113 crude oil degrading isolates 15 isolates were selected and cultivated in BH media with 1% crude oil as a sole carbon and energy source. 3 efficient crude oil bacterial isolates Bacillus subtilis I1, Pseudomonas aeruginosa I5 and Pseudomonas putida I8 were identified both biochemically and phylogenetically.The quantitative analysis of biodegradation is carried out gravimetrically and highest degradation rate, 55% was recorded by Pseudomonas aeruginosa I5 isolate.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, SRM Arts & Science College, Kattangulathur - 603203, Kancheepuram Dist, Tamilnadu, India.

ABSTRACT
The widespread problem caused due to petroleum products, is their discharge and accidental spillage in marine environment proving to be hazardous to the surroundings as well as life forms. Thus remediation of these hydrocarbons by natural decontamination process is of utmost importance. Bioremediation is a non-invasive and cost effective technique for the clean-up of these petroleum hydrocarbons. In this study we have investigated the ability of microorganisms present in the sediment sample to degrade these hydrocarbons, crude oil in particular, so that contaminated soils and water can be treated using microbes. Sediments samples were collected once in a month for a period of twelve months from area surrounding Ennore creek and screened for hydrocarbon degrading bacteria. Of the 113 crude oil degrading isolates 15 isolates were selected and cultivated in BH media with 1% crude oil as a sole carbon and energy source. 3 efficient crude oil bacterial isolates Bacillus subtilis I1, Pseudomonas aeruginosa I5 and Pseudomonas putida I8 were identified both biochemically and phylogenetically. The quantitative analysis of biodegradation is carried out gravimetrically and highest degradation rate, 55% was recorded by Pseudomonas aeruginosa I5 isolate.

No MeSH data available.


Related in: MedlinePlus