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Isolation and Identification of hydrocarbon degrading bacteria from Ennore creek.

Subathra MK, Immanuel G, Suresh AH - Bioinformation (2013)

Bottom Line: Bioremediation is a non-invasive and cost effective technique for the clean-up of these petroleum hydrocarbons.Of the 113 crude oil degrading isolates 15 isolates were selected and cultivated in BH media with 1% crude oil as a sole carbon and energy source. 3 efficient crude oil bacterial isolates Bacillus subtilis I1, Pseudomonas aeruginosa I5 and Pseudomonas putida I8 were identified both biochemically and phylogenetically.The quantitative analysis of biodegradation is carried out gravimetrically and highest degradation rate, 55% was recorded by Pseudomonas aeruginosa I5 isolate.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, SRM Arts & Science College, Kattangulathur - 603203, Kancheepuram Dist, Tamilnadu, India.

ABSTRACT
The widespread problem caused due to petroleum products, is their discharge and accidental spillage in marine environment proving to be hazardous to the surroundings as well as life forms. Thus remediation of these hydrocarbons by natural decontamination process is of utmost importance. Bioremediation is a non-invasive and cost effective technique for the clean-up of these petroleum hydrocarbons. In this study we have investigated the ability of microorganisms present in the sediment sample to degrade these hydrocarbons, crude oil in particular, so that contaminated soils and water can be treated using microbes. Sediments samples were collected once in a month for a period of twelve months from area surrounding Ennore creek and screened for hydrocarbon degrading bacteria. Of the 113 crude oil degrading isolates 15 isolates were selected and cultivated in BH media with 1% crude oil as a sole carbon and energy source. 3 efficient crude oil bacterial isolates Bacillus subtilis I1, Pseudomonas aeruginosa I5 and Pseudomonas putida I8 were identified both biochemically and phylogenetically. The quantitative analysis of biodegradation is carried out gravimetrically and highest degradation rate, 55% was recorded by Pseudomonas aeruginosa I5 isolate.

No MeSH data available.


Related in: MedlinePlus

16s RNA partial sequences.
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Related In: Results  -  Collection


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Figure 5: 16s RNA partial sequences.

Mentions: The 16s RNA partial sequencing (Figure 5) of the 3 differentbacterial generae viz., 1 Bacillus sp. and 2 Pseudomonas sp. hadshown that the organisms were identified as B. subtilis (I1), P.aeruginosa (I5) and P. putida (I8). The sequences aligned with theBLAST search of NCBI data bases were found to show 98%similarity for all the three strains. Bacterial strain PS-I isolatedfrom soil samples collected from oil production site of Oil andNatural Gas Commission (ONGC) was identified as P. putidawith 97% similarity by 16s rDNA analysis with blast search ofNCBI, RDP and microseqTM [16]. Studies on 16s RNA genesequence of the isolate VITDDK3 from Ennore creek werealigned with ClustW data base and the strain was designated asStreptomyces sp [17].


Isolation and Identification of hydrocarbon degrading bacteria from Ennore creek.

Subathra MK, Immanuel G, Suresh AH - Bioinformation (2013)

16s RNA partial sequences.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3569603&req=5

Figure 5: 16s RNA partial sequences.
Mentions: The 16s RNA partial sequencing (Figure 5) of the 3 differentbacterial generae viz., 1 Bacillus sp. and 2 Pseudomonas sp. hadshown that the organisms were identified as B. subtilis (I1), P.aeruginosa (I5) and P. putida (I8). The sequences aligned with theBLAST search of NCBI data bases were found to show 98%similarity for all the three strains. Bacterial strain PS-I isolatedfrom soil samples collected from oil production site of Oil andNatural Gas Commission (ONGC) was identified as P. putidawith 97% similarity by 16s rDNA analysis with blast search ofNCBI, RDP and microseqTM [16]. Studies on 16s RNA genesequence of the isolate VITDDK3 from Ennore creek werealigned with ClustW data base and the strain was designated asStreptomyces sp [17].

Bottom Line: Bioremediation is a non-invasive and cost effective technique for the clean-up of these petroleum hydrocarbons.Of the 113 crude oil degrading isolates 15 isolates were selected and cultivated in BH media with 1% crude oil as a sole carbon and energy source. 3 efficient crude oil bacterial isolates Bacillus subtilis I1, Pseudomonas aeruginosa I5 and Pseudomonas putida I8 were identified both biochemically and phylogenetically.The quantitative analysis of biodegradation is carried out gravimetrically and highest degradation rate, 55% was recorded by Pseudomonas aeruginosa I5 isolate.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, SRM Arts & Science College, Kattangulathur - 603203, Kancheepuram Dist, Tamilnadu, India.

ABSTRACT
The widespread problem caused due to petroleum products, is their discharge and accidental spillage in marine environment proving to be hazardous to the surroundings as well as life forms. Thus remediation of these hydrocarbons by natural decontamination process is of utmost importance. Bioremediation is a non-invasive and cost effective technique for the clean-up of these petroleum hydrocarbons. In this study we have investigated the ability of microorganisms present in the sediment sample to degrade these hydrocarbons, crude oil in particular, so that contaminated soils and water can be treated using microbes. Sediments samples were collected once in a month for a period of twelve months from area surrounding Ennore creek and screened for hydrocarbon degrading bacteria. Of the 113 crude oil degrading isolates 15 isolates were selected and cultivated in BH media with 1% crude oil as a sole carbon and energy source. 3 efficient crude oil bacterial isolates Bacillus subtilis I1, Pseudomonas aeruginosa I5 and Pseudomonas putida I8 were identified both biochemically and phylogenetically. The quantitative analysis of biodegradation is carried out gravimetrically and highest degradation rate, 55% was recorded by Pseudomonas aeruginosa I5 isolate.

No MeSH data available.


Related in: MedlinePlus