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Isolation and Identification of hydrocarbon degrading bacteria from Ennore creek.

Subathra MK, Immanuel G, Suresh AH - Bioinformation (2013)

Bottom Line: Bioremediation is a non-invasive and cost effective technique for the clean-up of these petroleum hydrocarbons.Of the 113 crude oil degrading isolates 15 isolates were selected and cultivated in BH media with 1% crude oil as a sole carbon and energy source. 3 efficient crude oil bacterial isolates Bacillus subtilis I1, Pseudomonas aeruginosa I5 and Pseudomonas putida I8 were identified both biochemically and phylogenetically.The quantitative analysis of biodegradation is carried out gravimetrically and highest degradation rate, 55% was recorded by Pseudomonas aeruginosa I5 isolate.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, SRM Arts & Science College, Kattangulathur - 603203, Kancheepuram Dist, Tamilnadu, India.

ABSTRACT
The widespread problem caused due to petroleum products, is their discharge and accidental spillage in marine environment proving to be hazardous to the surroundings as well as life forms. Thus remediation of these hydrocarbons by natural decontamination process is of utmost importance. Bioremediation is a non-invasive and cost effective technique for the clean-up of these petroleum hydrocarbons. In this study we have investigated the ability of microorganisms present in the sediment sample to degrade these hydrocarbons, crude oil in particular, so that contaminated soils and water can be treated using microbes. Sediments samples were collected once in a month for a period of twelve months from area surrounding Ennore creek and screened for hydrocarbon degrading bacteria. Of the 113 crude oil degrading isolates 15 isolates were selected and cultivated in BH media with 1% crude oil as a sole carbon and energy source. 3 efficient crude oil bacterial isolates Bacillus subtilis I1, Pseudomonas aeruginosa I5 and Pseudomonas putida I8 were identified both biochemically and phylogenetically. The quantitative analysis of biodegradation is carried out gravimetrically and highest degradation rate, 55% was recorded by Pseudomonas aeruginosa I5 isolate.

No MeSH data available.


Related in: MedlinePlus

Percentage of zone of clearance on BHA plates byhydrocarbon degraders.
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Related In: Results  -  Collection


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Figure 3: Percentage of zone of clearance on BHA plates byhydrocarbon degraders.

Mentions: 15 isolates of 113 hydrocarbon degraders, were found tomaintain its crude oil degrading ability, which was establishedby the isolates growing both on BHA with 100µl of crude oiland on NA plates. The efficiency to degrade was recorded bythe zone of clearance exhibited by the degraders on BHA paltes.The zone of clearance, shown a minimum of 2 mm, by Serratiasp and a maximum of 11mm, by Pseudomonas sp. Pseudomonas spfound to predominate the group by 40%, followed by Bacillussp. 26.67%, Micrococcus sp. by 20% and lastly by Serratia sp. andAchromobacter sp. by 6.7%. Five isolates, I1, I2, I5, I8 and I14were found to exhibit maximum clearing zone, Table 4 (seesupplementary material) & (Figure 3) compared to theremaining isolates.


Isolation and Identification of hydrocarbon degrading bacteria from Ennore creek.

Subathra MK, Immanuel G, Suresh AH - Bioinformation (2013)

Percentage of zone of clearance on BHA plates byhydrocarbon degraders.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3569603&req=5

Figure 3: Percentage of zone of clearance on BHA plates byhydrocarbon degraders.
Mentions: 15 isolates of 113 hydrocarbon degraders, were found tomaintain its crude oil degrading ability, which was establishedby the isolates growing both on BHA with 100µl of crude oiland on NA plates. The efficiency to degrade was recorded bythe zone of clearance exhibited by the degraders on BHA paltes.The zone of clearance, shown a minimum of 2 mm, by Serratiasp and a maximum of 11mm, by Pseudomonas sp. Pseudomonas spfound to predominate the group by 40%, followed by Bacillussp. 26.67%, Micrococcus sp. by 20% and lastly by Serratia sp. andAchromobacter sp. by 6.7%. Five isolates, I1, I2, I5, I8 and I14were found to exhibit maximum clearing zone, Table 4 (seesupplementary material) & (Figure 3) compared to theremaining isolates.

Bottom Line: Bioremediation is a non-invasive and cost effective technique for the clean-up of these petroleum hydrocarbons.Of the 113 crude oil degrading isolates 15 isolates were selected and cultivated in BH media with 1% crude oil as a sole carbon and energy source. 3 efficient crude oil bacterial isolates Bacillus subtilis I1, Pseudomonas aeruginosa I5 and Pseudomonas putida I8 were identified both biochemically and phylogenetically.The quantitative analysis of biodegradation is carried out gravimetrically and highest degradation rate, 55% was recorded by Pseudomonas aeruginosa I5 isolate.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, SRM Arts & Science College, Kattangulathur - 603203, Kancheepuram Dist, Tamilnadu, India.

ABSTRACT
The widespread problem caused due to petroleum products, is their discharge and accidental spillage in marine environment proving to be hazardous to the surroundings as well as life forms. Thus remediation of these hydrocarbons by natural decontamination process is of utmost importance. Bioremediation is a non-invasive and cost effective technique for the clean-up of these petroleum hydrocarbons. In this study we have investigated the ability of microorganisms present in the sediment sample to degrade these hydrocarbons, crude oil in particular, so that contaminated soils and water can be treated using microbes. Sediments samples were collected once in a month for a period of twelve months from area surrounding Ennore creek and screened for hydrocarbon degrading bacteria. Of the 113 crude oil degrading isolates 15 isolates were selected and cultivated in BH media with 1% crude oil as a sole carbon and energy source. 3 efficient crude oil bacterial isolates Bacillus subtilis I1, Pseudomonas aeruginosa I5 and Pseudomonas putida I8 were identified both biochemically and phylogenetically. The quantitative analysis of biodegradation is carried out gravimetrically and highest degradation rate, 55% was recorded by Pseudomonas aeruginosa I5 isolate.

No MeSH data available.


Related in: MedlinePlus