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Isolation and Identification of hydrocarbon degrading bacteria from Ennore creek.

Subathra MK, Immanuel G, Suresh AH - Bioinformation (2013)

Bottom Line: Bioremediation is a non-invasive and cost effective technique for the clean-up of these petroleum hydrocarbons.Of the 113 crude oil degrading isolates 15 isolates were selected and cultivated in BH media with 1% crude oil as a sole carbon and energy source. 3 efficient crude oil bacterial isolates Bacillus subtilis I1, Pseudomonas aeruginosa I5 and Pseudomonas putida I8 were identified both biochemically and phylogenetically.The quantitative analysis of biodegradation is carried out gravimetrically and highest degradation rate, 55% was recorded by Pseudomonas aeruginosa I5 isolate.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, SRM Arts & Science College, Kattangulathur - 603203, Kancheepuram Dist, Tamilnadu, India.

ABSTRACT
The widespread problem caused due to petroleum products, is their discharge and accidental spillage in marine environment proving to be hazardous to the surroundings as well as life forms. Thus remediation of these hydrocarbons by natural decontamination process is of utmost importance. Bioremediation is a non-invasive and cost effective technique for the clean-up of these petroleum hydrocarbons. In this study we have investigated the ability of microorganisms present in the sediment sample to degrade these hydrocarbons, crude oil in particular, so that contaminated soils and water can be treated using microbes. Sediments samples were collected once in a month for a period of twelve months from area surrounding Ennore creek and screened for hydrocarbon degrading bacteria. Of the 113 crude oil degrading isolates 15 isolates were selected and cultivated in BH media with 1% crude oil as a sole carbon and energy source. 3 efficient crude oil bacterial isolates Bacillus subtilis I1, Pseudomonas aeruginosa I5 and Pseudomonas putida I8 were identified both biochemically and phylogenetically. The quantitative analysis of biodegradation is carried out gravimetrically and highest degradation rate, 55% was recorded by Pseudomonas aeruginosa I5 isolate.

No MeSH data available.


Related in: MedlinePlus

(A) Total heterotrophic bacterial population; (B) Totalhydrocarbon degrading bacterial population; (C) Percentage ofhydrocarbon degraders.
© Copyright Policy - open-access
Related In: Results  -  Collection


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Figure 1: (A) Total heterotrophic bacterial population; (B) Totalhydrocarbon degrading bacterial population; (C) Percentage ofhydrocarbon degraders.

Mentions: The average number of heterotrophic bacteria during the studyperiod was in the order of 22.32× 105 CFUg-1, with the highestcount 32.4×105 CFUg-1 being recorded in the post monsoon,August, 2009 and the lowest 12.21×104 CFUg-1 in the beginningof summer, March and April, 2010 Table 1 (see supplementarymaterial) & (Figure 1A). Similar studies recorded that the THBcounts ranged from 9.0×103 to 2.6×106 CFUg-1 in thehydrocarbon contaminated surface sediment samples ofGokana, River state [9]. THB population ranged from 105 to9.0×108 CFUg-1 on sediment samples collected from Cuddalorefishing harbor over a period of 8 months from October 2011onwards was reported [10].


Isolation and Identification of hydrocarbon degrading bacteria from Ennore creek.

Subathra MK, Immanuel G, Suresh AH - Bioinformation (2013)

(A) Total heterotrophic bacterial population; (B) Totalhydrocarbon degrading bacterial population; (C) Percentage ofhydrocarbon degraders.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3569603&req=5

Figure 1: (A) Total heterotrophic bacterial population; (B) Totalhydrocarbon degrading bacterial population; (C) Percentage ofhydrocarbon degraders.
Mentions: The average number of heterotrophic bacteria during the studyperiod was in the order of 22.32× 105 CFUg-1, with the highestcount 32.4×105 CFUg-1 being recorded in the post monsoon,August, 2009 and the lowest 12.21×104 CFUg-1 in the beginningof summer, March and April, 2010 Table 1 (see supplementarymaterial) & (Figure 1A). Similar studies recorded that the THBcounts ranged from 9.0×103 to 2.6×106 CFUg-1 in thehydrocarbon contaminated surface sediment samples ofGokana, River state [9]. THB population ranged from 105 to9.0×108 CFUg-1 on sediment samples collected from Cuddalorefishing harbor over a period of 8 months from October 2011onwards was reported [10].

Bottom Line: Bioremediation is a non-invasive and cost effective technique for the clean-up of these petroleum hydrocarbons.Of the 113 crude oil degrading isolates 15 isolates were selected and cultivated in BH media with 1% crude oil as a sole carbon and energy source. 3 efficient crude oil bacterial isolates Bacillus subtilis I1, Pseudomonas aeruginosa I5 and Pseudomonas putida I8 were identified both biochemically and phylogenetically.The quantitative analysis of biodegradation is carried out gravimetrically and highest degradation rate, 55% was recorded by Pseudomonas aeruginosa I5 isolate.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, SRM Arts & Science College, Kattangulathur - 603203, Kancheepuram Dist, Tamilnadu, India.

ABSTRACT
The widespread problem caused due to petroleum products, is their discharge and accidental spillage in marine environment proving to be hazardous to the surroundings as well as life forms. Thus remediation of these hydrocarbons by natural decontamination process is of utmost importance. Bioremediation is a non-invasive and cost effective technique for the clean-up of these petroleum hydrocarbons. In this study we have investigated the ability of microorganisms present in the sediment sample to degrade these hydrocarbons, crude oil in particular, so that contaminated soils and water can be treated using microbes. Sediments samples were collected once in a month for a period of twelve months from area surrounding Ennore creek and screened for hydrocarbon degrading bacteria. Of the 113 crude oil degrading isolates 15 isolates were selected and cultivated in BH media with 1% crude oil as a sole carbon and energy source. 3 efficient crude oil bacterial isolates Bacillus subtilis I1, Pseudomonas aeruginosa I5 and Pseudomonas putida I8 were identified both biochemically and phylogenetically. The quantitative analysis of biodegradation is carried out gravimetrically and highest degradation rate, 55% was recorded by Pseudomonas aeruginosa I5 isolate.

No MeSH data available.


Related in: MedlinePlus