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Screening of recombinant proteins as antigens in indirect ELISA for diagnosis of bovine tuberculosis.

Souza II, Melo ES, Ramos CA, Farias TA, Osório AL, Jorge KS, Vidal CE, Silva AS, Silva MR, Pellegrin AO, Araújo FR - Springerplus (2012)

Bottom Line: Control programs in most countries, including Brazil, are based on the identification and slaughter of infected animals, as defined by the skin tuberculin test, which has its constraints.Also, 236 sera from two BTB-free beef cattle herds were tested.Among the proteins tested, only the ESAT-6/MPB70/MPB83 chimera demonstrated satisfactory agreement with the CITT (kappa index: 0.688), reflecting in 83.2% sensitivity and 86.5% specificity.

View Article: PubMed Central - PubMed

Affiliation: Pós Graduação em Ciência Animal, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS Brazil.

ABSTRACT
Bovine tuberculosis is an important infectious disease caused by Mycobacterium bovis, which is responsible for considerable economic losses. This disease constitutes a serious public health problem. Control programs in most countries, including Brazil, are based on the identification and slaughter of infected animals, as defined by the skin tuberculin test, which has its constraints. In the present study, the recombinant proteins CFP-10, ESAT-6, Mb0143, MPB83, PE5, PE13, TB10.4, TB15.3 and a chimera of ESAT-6/MPB70/MPB83 (fusion protein) were tested as ELISA antigens for the diagnosis of bovine tuberculosis. The proteins were produced in Escherichia coli, purified and tested in ELISAs with sera from 126 cattle having tested negative in the comparative intradermal tuberculin test (CITT) and 107 sera from cattle having tested positive in the CITT. Also, 236 sera from two BTB-free beef cattle herds were tested. Among the proteins tested, only the ESAT-6/MPB70/MPB83 chimera demonstrated satisfactory agreement with the CITT (kappa index: 0.688), reflecting in 83.2% sensitivity and 86.5% specificity. The ELISA absorbances of the cattle sera from BTB-free herds showed similar levels to those of CITT positive cattle, probably as the result of successive skin tuberculinizations to define the BTB-free status of the herds. However, the ELISA with the ESAT-6/MPB70/MPB83 chimera was useful to discriminate BTB positive and negative cattle in herds prior to the tuberculin skin test.

No MeSH data available.


Related in: MedlinePlus

Dot-plot showing normalized ELISA absorbance of sera from CITT negative, CITT positive cattle, BTB-free beef cattle and dairy cattle herds. The chimera antigen (MPB70/MPB83/ESAT-6) alone was used as a model in this figure, as it provided better differentiation between positive or negative cattle for tuberculosis.
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Fig2: Dot-plot showing normalized ELISA absorbance of sera from CITT negative, CITT positive cattle, BTB-free beef cattle and dairy cattle herds. The chimera antigen (MPB70/MPB83/ESAT-6) alone was used as a model in this figure, as it provided better differentiation between positive or negative cattle for tuberculosis.

Mentions: The normalized ELISA absorbances of the cattle sera from BTB-free herds showed similar levels to those of CITT positive cattle (Figure 2).Figure 2


Screening of recombinant proteins as antigens in indirect ELISA for diagnosis of bovine tuberculosis.

Souza II, Melo ES, Ramos CA, Farias TA, Osório AL, Jorge KS, Vidal CE, Silva AS, Silva MR, Pellegrin AO, Araújo FR - Springerplus (2012)

Dot-plot showing normalized ELISA absorbance of sera from CITT negative, CITT positive cattle, BTB-free beef cattle and dairy cattle herds. The chimera antigen (MPB70/MPB83/ESAT-6) alone was used as a model in this figure, as it provided better differentiation between positive or negative cattle for tuberculosis.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3569591&req=5

Fig2: Dot-plot showing normalized ELISA absorbance of sera from CITT negative, CITT positive cattle, BTB-free beef cattle and dairy cattle herds. The chimera antigen (MPB70/MPB83/ESAT-6) alone was used as a model in this figure, as it provided better differentiation between positive or negative cattle for tuberculosis.
Mentions: The normalized ELISA absorbances of the cattle sera from BTB-free herds showed similar levels to those of CITT positive cattle (Figure 2).Figure 2

Bottom Line: Control programs in most countries, including Brazil, are based on the identification and slaughter of infected animals, as defined by the skin tuberculin test, which has its constraints.Also, 236 sera from two BTB-free beef cattle herds were tested.Among the proteins tested, only the ESAT-6/MPB70/MPB83 chimera demonstrated satisfactory agreement with the CITT (kappa index: 0.688), reflecting in 83.2% sensitivity and 86.5% specificity.

View Article: PubMed Central - PubMed

Affiliation: Pós Graduação em Ciência Animal, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS Brazil.

ABSTRACT
Bovine tuberculosis is an important infectious disease caused by Mycobacterium bovis, which is responsible for considerable economic losses. This disease constitutes a serious public health problem. Control programs in most countries, including Brazil, are based on the identification and slaughter of infected animals, as defined by the skin tuberculin test, which has its constraints. In the present study, the recombinant proteins CFP-10, ESAT-6, Mb0143, MPB83, PE5, PE13, TB10.4, TB15.3 and a chimera of ESAT-6/MPB70/MPB83 (fusion protein) were tested as ELISA antigens for the diagnosis of bovine tuberculosis. The proteins were produced in Escherichia coli, purified and tested in ELISAs with sera from 126 cattle having tested negative in the comparative intradermal tuberculin test (CITT) and 107 sera from cattle having tested positive in the CITT. Also, 236 sera from two BTB-free beef cattle herds were tested. Among the proteins tested, only the ESAT-6/MPB70/MPB83 chimera demonstrated satisfactory agreement with the CITT (kappa index: 0.688), reflecting in 83.2% sensitivity and 86.5% specificity. The ELISA absorbances of the cattle sera from BTB-free herds showed similar levels to those of CITT positive cattle, probably as the result of successive skin tuberculinizations to define the BTB-free status of the herds. However, the ELISA with the ESAT-6/MPB70/MPB83 chimera was useful to discriminate BTB positive and negative cattle in herds prior to the tuberculin skin test.

No MeSH data available.


Related in: MedlinePlus