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Screening of recombinant proteins as antigens in indirect ELISA for diagnosis of bovine tuberculosis.

Souza II, Melo ES, Ramos CA, Farias TA, Osório AL, Jorge KS, Vidal CE, Silva AS, Silva MR, Pellegrin AO, Araújo FR - Springerplus (2012)

Bottom Line: Control programs in most countries, including Brazil, are based on the identification and slaughter of infected animals, as defined by the skin tuberculin test, which has its constraints.Also, 236 sera from two BTB-free beef cattle herds were tested.Among the proteins tested, only the ESAT-6/MPB70/MPB83 chimera demonstrated satisfactory agreement with the CITT (kappa index: 0.688), reflecting in 83.2% sensitivity and 86.5% specificity.

View Article: PubMed Central - PubMed

Affiliation: Pós Graduação em Ciência Animal, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS Brazil.

ABSTRACT
Bovine tuberculosis is an important infectious disease caused by Mycobacterium bovis, which is responsible for considerable economic losses. This disease constitutes a serious public health problem. Control programs in most countries, including Brazil, are based on the identification and slaughter of infected animals, as defined by the skin tuberculin test, which has its constraints. In the present study, the recombinant proteins CFP-10, ESAT-6, Mb0143, MPB83, PE5, PE13, TB10.4, TB15.3 and a chimera of ESAT-6/MPB70/MPB83 (fusion protein) were tested as ELISA antigens for the diagnosis of bovine tuberculosis. The proteins were produced in Escherichia coli, purified and tested in ELISAs with sera from 126 cattle having tested negative in the comparative intradermal tuberculin test (CITT) and 107 sera from cattle having tested positive in the CITT. Also, 236 sera from two BTB-free beef cattle herds were tested. Among the proteins tested, only the ESAT-6/MPB70/MPB83 chimera demonstrated satisfactory agreement with the CITT (kappa index: 0.688), reflecting in 83.2% sensitivity and 86.5% specificity. The ELISA absorbances of the cattle sera from BTB-free herds showed similar levels to those of CITT positive cattle, probably as the result of successive skin tuberculinizations to define the BTB-free status of the herds. However, the ELISA with the ESAT-6/MPB70/MPB83 chimera was useful to discriminate BTB positive and negative cattle in herds prior to the tuberculin skin test.

No MeSH data available.


Related in: MedlinePlus

Distributions of normalized absorbances of ELISAs with recombinant proteins ofMycobacterium bovis.
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Fig1: Distributions of normalized absorbances of ELISAs with recombinant proteins ofMycobacterium bovis.

Mentions: Figure 1 displays the normalized ELISA absorbances. Results are expressed as relative sensitivity or relative specificity taking CITT as the reference test. Relative sensitivity ranged from 28.0% to 83.2% and relative specificity ranged from 58.7% to 92.9%. The highest relative specificity (92.9%) of the ELISA with CFP-10 was in detriment to the relative sensitivity of the test (28.0%).Figure 1


Screening of recombinant proteins as antigens in indirect ELISA for diagnosis of bovine tuberculosis.

Souza II, Melo ES, Ramos CA, Farias TA, Osório AL, Jorge KS, Vidal CE, Silva AS, Silva MR, Pellegrin AO, Araújo FR - Springerplus (2012)

Distributions of normalized absorbances of ELISAs with recombinant proteins ofMycobacterium bovis.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3569591&req=5

Fig1: Distributions of normalized absorbances of ELISAs with recombinant proteins ofMycobacterium bovis.
Mentions: Figure 1 displays the normalized ELISA absorbances. Results are expressed as relative sensitivity or relative specificity taking CITT as the reference test. Relative sensitivity ranged from 28.0% to 83.2% and relative specificity ranged from 58.7% to 92.9%. The highest relative specificity (92.9%) of the ELISA with CFP-10 was in detriment to the relative sensitivity of the test (28.0%).Figure 1

Bottom Line: Control programs in most countries, including Brazil, are based on the identification and slaughter of infected animals, as defined by the skin tuberculin test, which has its constraints.Also, 236 sera from two BTB-free beef cattle herds were tested.Among the proteins tested, only the ESAT-6/MPB70/MPB83 chimera demonstrated satisfactory agreement with the CITT (kappa index: 0.688), reflecting in 83.2% sensitivity and 86.5% specificity.

View Article: PubMed Central - PubMed

Affiliation: Pós Graduação em Ciência Animal, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS Brazil.

ABSTRACT
Bovine tuberculosis is an important infectious disease caused by Mycobacterium bovis, which is responsible for considerable economic losses. This disease constitutes a serious public health problem. Control programs in most countries, including Brazil, are based on the identification and slaughter of infected animals, as defined by the skin tuberculin test, which has its constraints. In the present study, the recombinant proteins CFP-10, ESAT-6, Mb0143, MPB83, PE5, PE13, TB10.4, TB15.3 and a chimera of ESAT-6/MPB70/MPB83 (fusion protein) were tested as ELISA antigens for the diagnosis of bovine tuberculosis. The proteins were produced in Escherichia coli, purified and tested in ELISAs with sera from 126 cattle having tested negative in the comparative intradermal tuberculin test (CITT) and 107 sera from cattle having tested positive in the CITT. Also, 236 sera from two BTB-free beef cattle herds were tested. Among the proteins tested, only the ESAT-6/MPB70/MPB83 chimera demonstrated satisfactory agreement with the CITT (kappa index: 0.688), reflecting in 83.2% sensitivity and 86.5% specificity. The ELISA absorbances of the cattle sera from BTB-free herds showed similar levels to those of CITT positive cattle, probably as the result of successive skin tuberculinizations to define the BTB-free status of the herds. However, the ELISA with the ESAT-6/MPB70/MPB83 chimera was useful to discriminate BTB positive and negative cattle in herds prior to the tuberculin skin test.

No MeSH data available.


Related in: MedlinePlus