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Comprehensive cytogenomic profile of the in vitro neuronal model SH-SY5Y.

Yusuf M, Leung K, Morris KJ, Volpi EV - Neurogenetics (2012)

Bottom Line: An important feature of the SH-SY5Y cells is their ability to differentiate into a functionally mature neuronal phenotype.This property has conferred them the potential to be used as an in vitro model for studies of neurodegenerative and neurodevelopmental disorders.Our results advocate for molecular cytogenetic data to inform the use of cancer cell lines in research.

View Article: PubMed Central - PubMed

Affiliation: Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, OX3 7BN, Oxford, UK.

ABSTRACT
The widely studied SH-SY5Y human neuroblastoma cell line provides a classic example of how a cancer cell line can be instrumental for discoveries of broad biological and clinical significance. An important feature of the SH-SY5Y cells is their ability to differentiate into a functionally mature neuronal phenotype. This property has conferred them the potential to be used as an in vitro model for studies of neurodegenerative and neurodevelopmental disorders. Here, we present a comprehensive assessment of the SH-SY5Y cytogenomic profile. Our results advocate for molecular cytogenetic data to inform the use of cancer cell lines in research.

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Related in: MedlinePlus

High-resolution multi-colour chromosome banding provides new clues on complex 1q rearrangement. A duplication of the entire long arm appears to have been followed by a paracentric inversion
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Related In: Results  -  Collection


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Fig3: High-resolution multi-colour chromosome banding provides new clues on complex 1q rearrangement. A duplication of the entire long arm appears to have been followed by a paracentric inversion

Mentions: Our investigations by high-resolution multi-colour chromosome banding on the long arm of chromosome 1 suggest a complex rearrangement arising from a duplication of the entire chromosome arm—as confirmed by our SNP-array analysis—followed by a paracentric inversion, resulting in a der(1)(1q12 → 1q31::1q31 → 1q12::1q44 → 1q31::1q31 → 1q44) (Fig. 3). Duplication of the juxtacentromeric heterochromatic area was confirmed by FISH analysis with a D1Z1 probe for the classical satellite DNA (data not shown).Fig. 3


Comprehensive cytogenomic profile of the in vitro neuronal model SH-SY5Y.

Yusuf M, Leung K, Morris KJ, Volpi EV - Neurogenetics (2012)

High-resolution multi-colour chromosome banding provides new clues on complex 1q rearrangement. A duplication of the entire long arm appears to have been followed by a paracentric inversion
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3569589&req=5

Fig3: High-resolution multi-colour chromosome banding provides new clues on complex 1q rearrangement. A duplication of the entire long arm appears to have been followed by a paracentric inversion
Mentions: Our investigations by high-resolution multi-colour chromosome banding on the long arm of chromosome 1 suggest a complex rearrangement arising from a duplication of the entire chromosome arm—as confirmed by our SNP-array analysis—followed by a paracentric inversion, resulting in a der(1)(1q12 → 1q31::1q31 → 1q12::1q44 → 1q31::1q31 → 1q44) (Fig. 3). Duplication of the juxtacentromeric heterochromatic area was confirmed by FISH analysis with a D1Z1 probe for the classical satellite DNA (data not shown).Fig. 3

Bottom Line: An important feature of the SH-SY5Y cells is their ability to differentiate into a functionally mature neuronal phenotype.This property has conferred them the potential to be used as an in vitro model for studies of neurodegenerative and neurodevelopmental disorders.Our results advocate for molecular cytogenetic data to inform the use of cancer cell lines in research.

View Article: PubMed Central - PubMed

Affiliation: Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, OX3 7BN, Oxford, UK.

ABSTRACT
The widely studied SH-SY5Y human neuroblastoma cell line provides a classic example of how a cancer cell line can be instrumental for discoveries of broad biological and clinical significance. An important feature of the SH-SY5Y cells is their ability to differentiate into a functionally mature neuronal phenotype. This property has conferred them the potential to be used as an in vitro model for studies of neurodegenerative and neurodevelopmental disorders. Here, we present a comprehensive assessment of the SH-SY5Y cytogenomic profile. Our results advocate for molecular cytogenetic data to inform the use of cancer cell lines in research.

Show MeSH
Related in: MedlinePlus