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Comprehensive cytogenomic profile of the in vitro neuronal model SH-SY5Y.

Yusuf M, Leung K, Morris KJ, Volpi EV - Neurogenetics (2012)

Bottom Line: An important feature of the SH-SY5Y cells is their ability to differentiate into a functionally mature neuronal phenotype.This property has conferred them the potential to be used as an in vitro model for studies of neurodegenerative and neurodevelopmental disorders.Our results advocate for molecular cytogenetic data to inform the use of cancer cell lines in research.

View Article: PubMed Central - PubMed

Affiliation: Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, OX3 7BN, Oxford, UK.

ABSTRACT
The widely studied SH-SY5Y human neuroblastoma cell line provides a classic example of how a cancer cell line can be instrumental for discoveries of broad biological and clinical significance. An important feature of the SH-SY5Y cells is their ability to differentiate into a functionally mature neuronal phenotype. This property has conferred them the potential to be used as an in vitro model for studies of neurodegenerative and neurodevelopmental disorders. Here, we present a comprehensive assessment of the SH-SY5Y cytogenomic profile. Our results advocate for molecular cytogenetic data to inform the use of cancer cell lines in research.

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Three-colour FISH shows a complex rearrangement involving chromosomes 15, 17 and 22. Three-colour FISH with directly labelled chromosome-specific “paints” for chromosome 15 (red), 17 (green) and 22 (blue) on reverse-DAPI banded chromosomes confirms the presence of a der(22)t(15;22) (top arrow) and a der(15)t(15;17;22) (bottom arrow)
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Fig2: Three-colour FISH shows a complex rearrangement involving chromosomes 15, 17 and 22. Three-colour FISH with directly labelled chromosome-specific “paints” for chromosome 15 (red), 17 (green) and 22 (blue) on reverse-DAPI banded chromosomes confirms the presence of a der(22)t(15;22) (top arrow) and a der(15)t(15;17;22) (bottom arrow)

Mentions: An intriguing finding emerging from our investigations was the nature of the rearrangement(s) involving chromosome 17 and 22, which were revealed to be more complex than previously reported. Structural abnormalities of chromosome 17 resulting in gain of material are the most frequent genetic abnormalities in neuroblastoma and powerful independent predictor of poor outcome, commonly found in primary tumours and cell lines. Our SNP array analysis showed in the SH-SY5Y cell line a gain on chromosome 17q (17q21.33-17q25.3) and two distinct losses on chromosome 22q (22q12.3 and 22q13.1-22q13.31). Differently from what reported previously, our M-FISH and validation FISH experiments with chromosome-specific probes revealed the derivative 22 to be the result of a translocation involving not chromosome 17, as initially thought, but chromosome 15. We also identified a der(15)t(15; 17; 22) (Figs. 1 and 2).Fig. 2


Comprehensive cytogenomic profile of the in vitro neuronal model SH-SY5Y.

Yusuf M, Leung K, Morris KJ, Volpi EV - Neurogenetics (2012)

Three-colour FISH shows a complex rearrangement involving chromosomes 15, 17 and 22. Three-colour FISH with directly labelled chromosome-specific “paints” for chromosome 15 (red), 17 (green) and 22 (blue) on reverse-DAPI banded chromosomes confirms the presence of a der(22)t(15;22) (top arrow) and a der(15)t(15;17;22) (bottom arrow)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3569589&req=5

Fig2: Three-colour FISH shows a complex rearrangement involving chromosomes 15, 17 and 22. Three-colour FISH with directly labelled chromosome-specific “paints” for chromosome 15 (red), 17 (green) and 22 (blue) on reverse-DAPI banded chromosomes confirms the presence of a der(22)t(15;22) (top arrow) and a der(15)t(15;17;22) (bottom arrow)
Mentions: An intriguing finding emerging from our investigations was the nature of the rearrangement(s) involving chromosome 17 and 22, which were revealed to be more complex than previously reported. Structural abnormalities of chromosome 17 resulting in gain of material are the most frequent genetic abnormalities in neuroblastoma and powerful independent predictor of poor outcome, commonly found in primary tumours and cell lines. Our SNP array analysis showed in the SH-SY5Y cell line a gain on chromosome 17q (17q21.33-17q25.3) and two distinct losses on chromosome 22q (22q12.3 and 22q13.1-22q13.31). Differently from what reported previously, our M-FISH and validation FISH experiments with chromosome-specific probes revealed the derivative 22 to be the result of a translocation involving not chromosome 17, as initially thought, but chromosome 15. We also identified a der(15)t(15; 17; 22) (Figs. 1 and 2).Fig. 2

Bottom Line: An important feature of the SH-SY5Y cells is their ability to differentiate into a functionally mature neuronal phenotype.This property has conferred them the potential to be used as an in vitro model for studies of neurodegenerative and neurodevelopmental disorders.Our results advocate for molecular cytogenetic data to inform the use of cancer cell lines in research.

View Article: PubMed Central - PubMed

Affiliation: Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, OX3 7BN, Oxford, UK.

ABSTRACT
The widely studied SH-SY5Y human neuroblastoma cell line provides a classic example of how a cancer cell line can be instrumental for discoveries of broad biological and clinical significance. An important feature of the SH-SY5Y cells is their ability to differentiate into a functionally mature neuronal phenotype. This property has conferred them the potential to be used as an in vitro model for studies of neurodegenerative and neurodevelopmental disorders. Here, we present a comprehensive assessment of the SH-SY5Y cytogenomic profile. Our results advocate for molecular cytogenetic data to inform the use of cancer cell lines in research.

Show MeSH
Related in: MedlinePlus