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Comprehensive cytogenomic profile of the in vitro neuronal model SH-SY5Y.

Yusuf M, Leung K, Morris KJ, Volpi EV - Neurogenetics (2012)

Bottom Line: An important feature of the SH-SY5Y cells is their ability to differentiate into a functionally mature neuronal phenotype.This property has conferred them the potential to be used as an in vitro model for studies of neurodegenerative and neurodevelopmental disorders.Our results advocate for molecular cytogenetic data to inform the use of cancer cell lines in research.

View Article: PubMed Central - PubMed

Affiliation: Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, OX3 7BN, Oxford, UK.

ABSTRACT
The widely studied SH-SY5Y human neuroblastoma cell line provides a classic example of how a cancer cell line can be instrumental for discoveries of broad biological and clinical significance. An important feature of the SH-SY5Y cells is their ability to differentiate into a functionally mature neuronal phenotype. This property has conferred them the potential to be used as an in vitro model for studies of neurodegenerative and neurodevelopmental disorders. Here, we present a comprehensive assessment of the SH-SY5Y cytogenomic profile. Our results advocate for molecular cytogenetic data to inform the use of cancer cell lines in research.

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Representative example of M-FISH analysis. The karyotypic identity of the SH-SY5Y cell line is confirmed by multi-colour karyotyping
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Fig1: Representative example of M-FISH analysis. The karyotypic identity of the SH-SY5Y cell line is confirmed by multi-colour karyotyping

Mentions: M-FISH analysis confirmed the presence of a set of previously reported chromosomal abnormalities that in combination define the cytogenetic identity of the SH-SY5Y cell line (Fig. 1), consisting of chromosome 7 trisomy, a duplication of the entire q arm of chromosome 1, a balanced translocation involving chromosomes 7 and 8, a derivative 9 t(2;9), and a derivative 22 believed to be the result a of a t(17;22). Another distinctive karyotypic trait of the SH-SY5Y is the lack of amplification of the N-myc proto-oncogene.Fig. 1


Comprehensive cytogenomic profile of the in vitro neuronal model SH-SY5Y.

Yusuf M, Leung K, Morris KJ, Volpi EV - Neurogenetics (2012)

Representative example of M-FISH analysis. The karyotypic identity of the SH-SY5Y cell line is confirmed by multi-colour karyotyping
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3569589&req=5

Fig1: Representative example of M-FISH analysis. The karyotypic identity of the SH-SY5Y cell line is confirmed by multi-colour karyotyping
Mentions: M-FISH analysis confirmed the presence of a set of previously reported chromosomal abnormalities that in combination define the cytogenetic identity of the SH-SY5Y cell line (Fig. 1), consisting of chromosome 7 trisomy, a duplication of the entire q arm of chromosome 1, a balanced translocation involving chromosomes 7 and 8, a derivative 9 t(2;9), and a derivative 22 believed to be the result a of a t(17;22). Another distinctive karyotypic trait of the SH-SY5Y is the lack of amplification of the N-myc proto-oncogene.Fig. 1

Bottom Line: An important feature of the SH-SY5Y cells is their ability to differentiate into a functionally mature neuronal phenotype.This property has conferred them the potential to be used as an in vitro model for studies of neurodegenerative and neurodevelopmental disorders.Our results advocate for molecular cytogenetic data to inform the use of cancer cell lines in research.

View Article: PubMed Central - PubMed

Affiliation: Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, OX3 7BN, Oxford, UK.

ABSTRACT
The widely studied SH-SY5Y human neuroblastoma cell line provides a classic example of how a cancer cell line can be instrumental for discoveries of broad biological and clinical significance. An important feature of the SH-SY5Y cells is their ability to differentiate into a functionally mature neuronal phenotype. This property has conferred them the potential to be used as an in vitro model for studies of neurodegenerative and neurodevelopmental disorders. Here, we present a comprehensive assessment of the SH-SY5Y cytogenomic profile. Our results advocate for molecular cytogenetic data to inform the use of cancer cell lines in research.

Show MeSH
Related in: MedlinePlus