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Using Candida oleophila as a biocontriol agens to prevent foodborne Escherichia coli O157 EHEC infections.

Wang Y, Wei A, Li H - Springerplus (2012)

Bottom Line: Escherichia coli O157:H7 (EHEC O157) is a serious pathogen causing haemorrhagic colitis.The results presented in this study indicated that EHEC O157 could survive and grow in wounded 'Fuji' apples and extensively proliferate.Water as a decontaminant was ineffective in reducing EHEC O157 in wounded apples.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041 People's Republic of China.

ABSTRACT
Escherichia coli O157:H7 (EHEC O157) is a serious pathogen causing haemorrhagic colitis. In this study, inactivation kinetics of inoculated EHEC O157 and response of EHEC O157 in apple wounds by different concentrations of C. oleophila was investigated. The results presented in this study indicated that EHEC O157 could survive and grow in wounded 'Fuji' apples and extensively proliferate. Water as a decontaminant was ineffective in reducing EHEC O157 in wounded apples. C. oleophila could be a viable method of controlling wound contaminated by this pathogen at room temperature.

No MeSH data available.


Related in: MedlinePlus

Concentration dynamics of EHEC O157 during storage at 25°C withC. oleophila.
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Fig2: Concentration dynamics of EHEC O157 during storage at 25°C withC. oleophila.

Mentions: Concentration dynamics of EHEC O157 in wounded apples during storage at 25°C after treatments by different concentration of C. oleophila is shown in Figure 2. Inoculum of C. oleophila showed a significant effect on development of EHEC O157. The higher concentration of C .oleophila inoculated, the smaller increase of EHEC O157 was. Compared with K8 (C. oleophila, 108 CFU/ml), K5 (C. oleophila, 105 CFU/ml), as applied in this study, did not result in effective reduction of EHEC O157 populations in the first 12 h, but was able to significantly (P < 0.05) impede growth of the pathogen at the following time. The maximum populations of EHEC O157 inoculated with K5, K6, K7 and K8 were 6.8, 6.5, 6.1, 6.0 CFU/wound after 4 days of storage at 25°C, respectively. So K7 (C. oleophila, 107 CFU/ml) could be most appropriate for prevention growth of EHEC O157 at room temperature.Figure 2


Using Candida oleophila as a biocontriol agens to prevent foodborne Escherichia coli O157 EHEC infections.

Wang Y, Wei A, Li H - Springerplus (2012)

Concentration dynamics of EHEC O157 during storage at 25°C withC. oleophila.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3569578&req=5

Fig2: Concentration dynamics of EHEC O157 during storage at 25°C withC. oleophila.
Mentions: Concentration dynamics of EHEC O157 in wounded apples during storage at 25°C after treatments by different concentration of C. oleophila is shown in Figure 2. Inoculum of C. oleophila showed a significant effect on development of EHEC O157. The higher concentration of C .oleophila inoculated, the smaller increase of EHEC O157 was. Compared with K8 (C. oleophila, 108 CFU/ml), K5 (C. oleophila, 105 CFU/ml), as applied in this study, did not result in effective reduction of EHEC O157 populations in the first 12 h, but was able to significantly (P < 0.05) impede growth of the pathogen at the following time. The maximum populations of EHEC O157 inoculated with K5, K6, K7 and K8 were 6.8, 6.5, 6.1, 6.0 CFU/wound after 4 days of storage at 25°C, respectively. So K7 (C. oleophila, 107 CFU/ml) could be most appropriate for prevention growth of EHEC O157 at room temperature.Figure 2

Bottom Line: Escherichia coli O157:H7 (EHEC O157) is a serious pathogen causing haemorrhagic colitis.The results presented in this study indicated that EHEC O157 could survive and grow in wounded 'Fuji' apples and extensively proliferate.Water as a decontaminant was ineffective in reducing EHEC O157 in wounded apples.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041 People's Republic of China.

ABSTRACT
Escherichia coli O157:H7 (EHEC O157) is a serious pathogen causing haemorrhagic colitis. In this study, inactivation kinetics of inoculated EHEC O157 and response of EHEC O157 in apple wounds by different concentrations of C. oleophila was investigated. The results presented in this study indicated that EHEC O157 could survive and grow in wounded 'Fuji' apples and extensively proliferate. Water as a decontaminant was ineffective in reducing EHEC O157 in wounded apples. C. oleophila could be a viable method of controlling wound contaminated by this pathogen at room temperature.

No MeSH data available.


Related in: MedlinePlus