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Anticancer and antimicrobial activities of some antioxidant-rich cameroonian medicinal plants.

Tamokou Jde D, Chouna JR, Fischer-Fodor E, Chereches G, Barbos O, Damian G, Benedec D, Duma M, Efouet AP, Wabo HK, Kuiate JR, Mot A, Silaghi-Dumitrescu R - PLoS ONE (2013)

Bottom Line: The AOA of emodin was significantly higher than that of 3-geranyloxyemodin and 2-geranylemodin for both TEAC and HAPX methods.The lowest IC(50) values (i.e., highest cytotoxicity) were found for the extracts of Vismia laurentii, Psorospermum febrifugum, Pentadesma butyracea and Ficus asperifolia.Emodin is more toxic compared to the whole extract, 3-geranyloxyemodin and 2-geranylemodin.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry and Chemical Engineering, Babes-Bolyai University, Cluj-Napoca, Romania.

ABSTRACT
Traditional remedies have a long-standing history in Cameroon and continue to provide useful and applicable tools for treating ailments. Here, the anticancer, antimicrobial and antioxidant activities of ten antioxidant-rich Cameroonian medicinal plants and of some of their isolated compounds are evaluated.The plant extracts were prepared by maceration in organic solvents. Fractionation of plant extract was performed by column chromatography and the structures of isolated compounds (emodin, 3-geranyloxyemodin, 2-geranylemodin) were confirmed spectroscopically. The antioxidant activity (AOA) was determined using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) bleaching method, the trolox equivalent antioxidant capacity (TEAC), and the hemoglobin ascorbate peroxidase activity inhibition (HAPX) assays. The anticancer activity was evaluated against A431 squamous epidermal carcinoma, WM35 melanoma, A2780 ovary carcinoma and cisplatin-resistant A2780cis cells, using a direct colorimetric assay. The total phenolic content in the extracts was determined spectrophotometrically by the Folin-Ciocalteu method. Rumex abyssinicus showed the best AOA among the three assays employed. The AOA of emodin was significantly higher than that of 3-geranyloxyemodin and 2-geranylemodin for both TEAC and HAPX methods. The lowest IC(50) values (i.e., highest cytotoxicity) were found for the extracts of Vismia laurentii, Psorospermum febrifugum, Pentadesma butyracea and Ficus asperifolia. The Ficus asperifolia and Psorospermum febrifugum extracts are selective against A2780cis ovary cells, a cell line which is resistant to the standard anticancer drug cisplatin. Emodin is more toxic compared to the whole extract, 3-geranyloxyemodin and 2-geranylemodin. Its selectivity against the platinum-resistant A2780cis cell line is highest. All of the extracts display antimicrobial activity, in some cases comparable to that of gentamycin.

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Comparison between Psorospermum febrifugum extract and emodines cytotoxicity.Differences between anticancer activity of Psorospermum febrifugum extract and its isolated compounds against four different cancer cell lines.
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pone-0055880-g013: Comparison between Psorospermum febrifugum extract and emodines cytotoxicity.Differences between anticancer activity of Psorospermum febrifugum extract and its isolated compounds against four different cancer cell lines.

Mentions: We also analyzed three pure compounds derived from Psorospermum febrifugum crude extract: emodin (1), 3-geranyloxyemodin (2) and 2-geranylemodin (3) (Table 3 and Figure 13). The three compounds display toxicity against cancer cells; the pattern of IC50 values distribution is different from the plant extract: emodin is more toxic compared to the whole extract, but less selective. 3-geranyloxyemodin is less toxic against the platinum-resistant A2780cis cell line and against dermal cell lines, and to some extent more toxic against A2780 cells (differences are statistically not significant). The activity of 2-geranylemodin is superior to that of the P. febrifugum extract in epidermal cell lines, and its antiproliferative capacity is slightly decreased in ovary cell lines, but the selectivity against the cisplatin-resistant A2780cis cells is maintained. These results illustrate similarities with the literature data as regards anticancer activity of emodines isolated from other plants [25]–[30]. Emodines separated from plant extract are responsible for the cytotoxicity of P. febrifugum, but probably they are not the only active constituents of this plant extract.


Anticancer and antimicrobial activities of some antioxidant-rich cameroonian medicinal plants.

Tamokou Jde D, Chouna JR, Fischer-Fodor E, Chereches G, Barbos O, Damian G, Benedec D, Duma M, Efouet AP, Wabo HK, Kuiate JR, Mot A, Silaghi-Dumitrescu R - PLoS ONE (2013)

Comparison between Psorospermum febrifugum extract and emodines cytotoxicity.Differences between anticancer activity of Psorospermum febrifugum extract and its isolated compounds against four different cancer cell lines.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3569468&req=5

pone-0055880-g013: Comparison between Psorospermum febrifugum extract and emodines cytotoxicity.Differences between anticancer activity of Psorospermum febrifugum extract and its isolated compounds against four different cancer cell lines.
Mentions: We also analyzed three pure compounds derived from Psorospermum febrifugum crude extract: emodin (1), 3-geranyloxyemodin (2) and 2-geranylemodin (3) (Table 3 and Figure 13). The three compounds display toxicity against cancer cells; the pattern of IC50 values distribution is different from the plant extract: emodin is more toxic compared to the whole extract, but less selective. 3-geranyloxyemodin is less toxic against the platinum-resistant A2780cis cell line and against dermal cell lines, and to some extent more toxic against A2780 cells (differences are statistically not significant). The activity of 2-geranylemodin is superior to that of the P. febrifugum extract in epidermal cell lines, and its antiproliferative capacity is slightly decreased in ovary cell lines, but the selectivity against the cisplatin-resistant A2780cis cells is maintained. These results illustrate similarities with the literature data as regards anticancer activity of emodines isolated from other plants [25]–[30]. Emodines separated from plant extract are responsible for the cytotoxicity of P. febrifugum, but probably they are not the only active constituents of this plant extract.

Bottom Line: The AOA of emodin was significantly higher than that of 3-geranyloxyemodin and 2-geranylemodin for both TEAC and HAPX methods.The lowest IC(50) values (i.e., highest cytotoxicity) were found for the extracts of Vismia laurentii, Psorospermum febrifugum, Pentadesma butyracea and Ficus asperifolia.Emodin is more toxic compared to the whole extract, 3-geranyloxyemodin and 2-geranylemodin.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry and Chemical Engineering, Babes-Bolyai University, Cluj-Napoca, Romania.

ABSTRACT
Traditional remedies have a long-standing history in Cameroon and continue to provide useful and applicable tools for treating ailments. Here, the anticancer, antimicrobial and antioxidant activities of ten antioxidant-rich Cameroonian medicinal plants and of some of their isolated compounds are evaluated.The plant extracts were prepared by maceration in organic solvents. Fractionation of plant extract was performed by column chromatography and the structures of isolated compounds (emodin, 3-geranyloxyemodin, 2-geranylemodin) were confirmed spectroscopically. The antioxidant activity (AOA) was determined using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) bleaching method, the trolox equivalent antioxidant capacity (TEAC), and the hemoglobin ascorbate peroxidase activity inhibition (HAPX) assays. The anticancer activity was evaluated against A431 squamous epidermal carcinoma, WM35 melanoma, A2780 ovary carcinoma and cisplatin-resistant A2780cis cells, using a direct colorimetric assay. The total phenolic content in the extracts was determined spectrophotometrically by the Folin-Ciocalteu method. Rumex abyssinicus showed the best AOA among the three assays employed. The AOA of emodin was significantly higher than that of 3-geranyloxyemodin and 2-geranylemodin for both TEAC and HAPX methods. The lowest IC(50) values (i.e., highest cytotoxicity) were found for the extracts of Vismia laurentii, Psorospermum febrifugum, Pentadesma butyracea and Ficus asperifolia. The Ficus asperifolia and Psorospermum febrifugum extracts are selective against A2780cis ovary cells, a cell line which is resistant to the standard anticancer drug cisplatin. Emodin is more toxic compared to the whole extract, 3-geranyloxyemodin and 2-geranylemodin. Its selectivity against the platinum-resistant A2780cis cell line is highest. All of the extracts display antimicrobial activity, in some cases comparable to that of gentamycin.

Show MeSH
Related in: MedlinePlus