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Anticancer and antimicrobial activities of some antioxidant-rich cameroonian medicinal plants.

Tamokou Jde D, Chouna JR, Fischer-Fodor E, Chereches G, Barbos O, Damian G, Benedec D, Duma M, Efouet AP, Wabo HK, Kuiate JR, Mot A, Silaghi-Dumitrescu R - PLoS ONE (2013)

Bottom Line: The AOA of emodin was significantly higher than that of 3-geranyloxyemodin and 2-geranylemodin for both TEAC and HAPX methods.The lowest IC(50) values (i.e., highest cytotoxicity) were found for the extracts of Vismia laurentii, Psorospermum febrifugum, Pentadesma butyracea and Ficus asperifolia.Emodin is more toxic compared to the whole extract, 3-geranyloxyemodin and 2-geranylemodin.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry and Chemical Engineering, Babes-Bolyai University, Cluj-Napoca, Romania.

ABSTRACT
Traditional remedies have a long-standing history in Cameroon and continue to provide useful and applicable tools for treating ailments. Here, the anticancer, antimicrobial and antioxidant activities of ten antioxidant-rich Cameroonian medicinal plants and of some of their isolated compounds are evaluated.The plant extracts were prepared by maceration in organic solvents. Fractionation of plant extract was performed by column chromatography and the structures of isolated compounds (emodin, 3-geranyloxyemodin, 2-geranylemodin) were confirmed spectroscopically. The antioxidant activity (AOA) was determined using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) bleaching method, the trolox equivalent antioxidant capacity (TEAC), and the hemoglobin ascorbate peroxidase activity inhibition (HAPX) assays. The anticancer activity was evaluated against A431 squamous epidermal carcinoma, WM35 melanoma, A2780 ovary carcinoma and cisplatin-resistant A2780cis cells, using a direct colorimetric assay. The total phenolic content in the extracts was determined spectrophotometrically by the Folin-Ciocalteu method. Rumex abyssinicus showed the best AOA among the three assays employed. The AOA of emodin was significantly higher than that of 3-geranyloxyemodin and 2-geranylemodin for both TEAC and HAPX methods. The lowest IC(50) values (i.e., highest cytotoxicity) were found for the extracts of Vismia laurentii, Psorospermum febrifugum, Pentadesma butyracea and Ficus asperifolia. The Ficus asperifolia and Psorospermum febrifugum extracts are selective against A2780cis ovary cells, a cell line which is resistant to the standard anticancer drug cisplatin. Emodin is more toxic compared to the whole extract, 3-geranyloxyemodin and 2-geranylemodin. Its selectivity against the platinum-resistant A2780cis cell line is highest. All of the extracts display antimicrobial activity, in some cases comparable to that of gentamycin.

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Antioxidant activity evaluated by DPPH method.Area under the kinetic curve of the DPPH bleaching by the test samples for the first 136 s. Bars represent the mean ± SD of three independent experiments carried out in triplicate. Letters a–d indicate significant differences between samples according to one way ANOVA and Waller Duncan test; p<0.05.
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pone-0055880-g003: Antioxidant activity evaluated by DPPH method.Area under the kinetic curve of the DPPH bleaching by the test samples for the first 136 s. Bars represent the mean ± SD of three independent experiments carried out in triplicate. Letters a–d indicate significant differences between samples according to one way ANOVA and Waller Duncan test; p<0.05.

Mentions: In this study, the AOA of the plant extracts and some of their isolated compounds were evaluated using three different methods - DPPH, TEAC and HAPX. The results obtained are summarized on Figures 3, 4, 5, 6. Rumex abyssinicus showed the best AOA among the three mechanisms used. For the DPPH method, the lowest area values corresponding to the most antioxidant substances were found for the extracts from Tectona grandis, Rumex abyssinicus, Pentadesma butyracea, Vismia laurentii, Dichrostachys glomerata and Rumex bequaertii while the highest area values (least activity) were those of Hibiscus asper and Psorospermum febrifugum (Figure 3). No significant differences were observed between the AOA of vitamin C (reference drug), emodin and its two derivatives (Figure 3).


Anticancer and antimicrobial activities of some antioxidant-rich cameroonian medicinal plants.

Tamokou Jde D, Chouna JR, Fischer-Fodor E, Chereches G, Barbos O, Damian G, Benedec D, Duma M, Efouet AP, Wabo HK, Kuiate JR, Mot A, Silaghi-Dumitrescu R - PLoS ONE (2013)

Antioxidant activity evaluated by DPPH method.Area under the kinetic curve of the DPPH bleaching by the test samples for the first 136 s. Bars represent the mean ± SD of three independent experiments carried out in triplicate. Letters a–d indicate significant differences between samples according to one way ANOVA and Waller Duncan test; p<0.05.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3569468&req=5

pone-0055880-g003: Antioxidant activity evaluated by DPPH method.Area under the kinetic curve of the DPPH bleaching by the test samples for the first 136 s. Bars represent the mean ± SD of three independent experiments carried out in triplicate. Letters a–d indicate significant differences between samples according to one way ANOVA and Waller Duncan test; p<0.05.
Mentions: In this study, the AOA of the plant extracts and some of their isolated compounds were evaluated using three different methods - DPPH, TEAC and HAPX. The results obtained are summarized on Figures 3, 4, 5, 6. Rumex abyssinicus showed the best AOA among the three mechanisms used. For the DPPH method, the lowest area values corresponding to the most antioxidant substances were found for the extracts from Tectona grandis, Rumex abyssinicus, Pentadesma butyracea, Vismia laurentii, Dichrostachys glomerata and Rumex bequaertii while the highest area values (least activity) were those of Hibiscus asper and Psorospermum febrifugum (Figure 3). No significant differences were observed between the AOA of vitamin C (reference drug), emodin and its two derivatives (Figure 3).

Bottom Line: The AOA of emodin was significantly higher than that of 3-geranyloxyemodin and 2-geranylemodin for both TEAC and HAPX methods.The lowest IC(50) values (i.e., highest cytotoxicity) were found for the extracts of Vismia laurentii, Psorospermum febrifugum, Pentadesma butyracea and Ficus asperifolia.Emodin is more toxic compared to the whole extract, 3-geranyloxyemodin and 2-geranylemodin.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry and Chemical Engineering, Babes-Bolyai University, Cluj-Napoca, Romania.

ABSTRACT
Traditional remedies have a long-standing history in Cameroon and continue to provide useful and applicable tools for treating ailments. Here, the anticancer, antimicrobial and antioxidant activities of ten antioxidant-rich Cameroonian medicinal plants and of some of their isolated compounds are evaluated.The plant extracts were prepared by maceration in organic solvents. Fractionation of plant extract was performed by column chromatography and the structures of isolated compounds (emodin, 3-geranyloxyemodin, 2-geranylemodin) were confirmed spectroscopically. The antioxidant activity (AOA) was determined using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) bleaching method, the trolox equivalent antioxidant capacity (TEAC), and the hemoglobin ascorbate peroxidase activity inhibition (HAPX) assays. The anticancer activity was evaluated against A431 squamous epidermal carcinoma, WM35 melanoma, A2780 ovary carcinoma and cisplatin-resistant A2780cis cells, using a direct colorimetric assay. The total phenolic content in the extracts was determined spectrophotometrically by the Folin-Ciocalteu method. Rumex abyssinicus showed the best AOA among the three assays employed. The AOA of emodin was significantly higher than that of 3-geranyloxyemodin and 2-geranylemodin for both TEAC and HAPX methods. The lowest IC(50) values (i.e., highest cytotoxicity) were found for the extracts of Vismia laurentii, Psorospermum febrifugum, Pentadesma butyracea and Ficus asperifolia. The Ficus asperifolia and Psorospermum febrifugum extracts are selective against A2780cis ovary cells, a cell line which is resistant to the standard anticancer drug cisplatin. Emodin is more toxic compared to the whole extract, 3-geranyloxyemodin and 2-geranylemodin. Its selectivity against the platinum-resistant A2780cis cell line is highest. All of the extracts display antimicrobial activity, in some cases comparable to that of gentamycin.

Show MeSH
Related in: MedlinePlus