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Microscopic-observation drug-susceptibility assay for the diagnosis of drug-resistant tuberculosis in Harare, Zimbabwe.

Makamure B, Mhaka J, Makumbirofa S, Mutetwa R, Mupfumi L, Mason P, Metcalfe JZ - PLoS ONE (2013)

Bottom Line: Among persons with known HIV infection status, 39/59 (66%) were HIV-infected.Overall MODS sensitivity for M. tuberculosis detection was 85% (95% CI, 69-95%) and specificity was 93% (95% CI, 84-98%); diagnostic accuracy did not significantly differ by HIV infection status.Of 33 specimens with concurrent DST results, sensitivity of the MODS assay for detection of resistance to isoniazid, rifampin, and MDR-TB was 88% (95% CI, 68-97%), 96% (95% CI, 79-100%), and 91% (95% CI, 72-99%), respectively; specificity was 89% (95% CI, 52-100%), 89% (95% CI, 52-100%), and 90% (95% CI, 56-100%), respectively.

View Article: PubMed Central - PubMed

Affiliation: Biomedical Research and Training Institute, Harare, Zimbabwe.

ABSTRACT

Introduction: Limited data exist on use of the microscopic-observation drug-susceptibility (MODS) assay among persons suspected of MDR-TB living in high HIV-prevalence settings.

Methods: We retrospectively reviewed available clinical and drug susceptibility data for drug-resistant TB suspects referred for culture and drug-susceptibility testing between April 1, 2011 and March 1, 2012. The diagnostic accuracy of MODS was estimated against a reference standard including Löwenstein-Jensen (LJ) media and manual liquid (BACTEC MGIT) culture. The accuracy of MODS drug-susceptibility testing (DST) was assessed against a reference standard absolute concentration method.

Results: One hundred thirty-eight sputum samples were collected from 99 drug-resistant TB suspects; in addition, six previously cultured MDR isolates were included for assessment of DST accuracy. Among persons with known HIV infection status, 39/59 (66%) were HIV-infected. Eighty-six percent of patients had a history of prior TB treatment, and 80% of individuals were on antituberculous treatment at the time of sample collection. M. tuberculosis was identified by reference standard culture among 34/98 (35%) MDR-TB suspects. Overall MODS sensitivity for M. tuberculosis detection was 85% (95% CI, 69-95%) and specificity was 93% (95% CI, 84-98%); diagnostic accuracy did not significantly differ by HIV infection status. Median time to positivity was significantly shorter for MODS (7 days; IQR 7-15 days) than MGIT (12 days; IQR 6-16 days) or LJ (28 days; IQR 21-35 days; p<0.001). Of 33 specimens with concurrent DST results, sensitivity of the MODS assay for detection of resistance to isoniazid, rifampin, and MDR-TB was 88% (95% CI, 68-97%), 96% (95% CI, 79-100%), and 91% (95% CI, 72-99%), respectively; specificity was 89% (95% CI, 52-100%), 89% (95% CI, 52-100%), and 90% (95% CI, 56-100%), respectively.

Conclusion: In a high HIV-prevalence setting, MODS diagnosed TB and drug-resistant TB with high sensitivity and shorter turnaround time compared with standard culture and DST methods.

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Study Flow Diagram.Definition of abbreviation: MDR TB = multidrug resistant tuberculosis; MODS = microscopic-observation drug-susceptibility. †Six additional previously cultured isolates of known MDR status were included for analysis of drug susceptibility testing only and are not included here.
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pone-0055872-g001: Study Flow Diagram.Definition of abbreviation: MDR TB = multidrug resistant tuberculosis; MODS = microscopic-observation drug-susceptibility. †Six additional previously cultured isolates of known MDR status were included for analysis of drug susceptibility testing only and are not included here.

Mentions: One hundred thirty-eight sputum samples were collected from 99 drug-resistant TB suspects, of whom 40% were female and the median age was 37 years (interquartile range [IQR]: 27–44). Retreatment category was available for 86 (87%) patients; 12/86 (14%) had no prior TB history but were contacts of known or suspected MDR-TB cases (Table 1). Among persons with known HIV status, 39/59 (66%) were HIV-infected. One patient was excluded from further analysis due to insufficient sample quantity for MODS (Figure 1).


Microscopic-observation drug-susceptibility assay for the diagnosis of drug-resistant tuberculosis in Harare, Zimbabwe.

Makamure B, Mhaka J, Makumbirofa S, Mutetwa R, Mupfumi L, Mason P, Metcalfe JZ - PLoS ONE (2013)

Study Flow Diagram.Definition of abbreviation: MDR TB = multidrug resistant tuberculosis; MODS = microscopic-observation drug-susceptibility. †Six additional previously cultured isolates of known MDR status were included for analysis of drug susceptibility testing only and are not included here.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3569460&req=5

pone-0055872-g001: Study Flow Diagram.Definition of abbreviation: MDR TB = multidrug resistant tuberculosis; MODS = microscopic-observation drug-susceptibility. †Six additional previously cultured isolates of known MDR status were included for analysis of drug susceptibility testing only and are not included here.
Mentions: One hundred thirty-eight sputum samples were collected from 99 drug-resistant TB suspects, of whom 40% were female and the median age was 37 years (interquartile range [IQR]: 27–44). Retreatment category was available for 86 (87%) patients; 12/86 (14%) had no prior TB history but were contacts of known or suspected MDR-TB cases (Table 1). Among persons with known HIV status, 39/59 (66%) were HIV-infected. One patient was excluded from further analysis due to insufficient sample quantity for MODS (Figure 1).

Bottom Line: Among persons with known HIV infection status, 39/59 (66%) were HIV-infected.Overall MODS sensitivity for M. tuberculosis detection was 85% (95% CI, 69-95%) and specificity was 93% (95% CI, 84-98%); diagnostic accuracy did not significantly differ by HIV infection status.Of 33 specimens with concurrent DST results, sensitivity of the MODS assay for detection of resistance to isoniazid, rifampin, and MDR-TB was 88% (95% CI, 68-97%), 96% (95% CI, 79-100%), and 91% (95% CI, 72-99%), respectively; specificity was 89% (95% CI, 52-100%), 89% (95% CI, 52-100%), and 90% (95% CI, 56-100%), respectively.

View Article: PubMed Central - PubMed

Affiliation: Biomedical Research and Training Institute, Harare, Zimbabwe.

ABSTRACT

Introduction: Limited data exist on use of the microscopic-observation drug-susceptibility (MODS) assay among persons suspected of MDR-TB living in high HIV-prevalence settings.

Methods: We retrospectively reviewed available clinical and drug susceptibility data for drug-resistant TB suspects referred for culture and drug-susceptibility testing between April 1, 2011 and March 1, 2012. The diagnostic accuracy of MODS was estimated against a reference standard including Löwenstein-Jensen (LJ) media and manual liquid (BACTEC MGIT) culture. The accuracy of MODS drug-susceptibility testing (DST) was assessed against a reference standard absolute concentration method.

Results: One hundred thirty-eight sputum samples were collected from 99 drug-resistant TB suspects; in addition, six previously cultured MDR isolates were included for assessment of DST accuracy. Among persons with known HIV infection status, 39/59 (66%) were HIV-infected. Eighty-six percent of patients had a history of prior TB treatment, and 80% of individuals were on antituberculous treatment at the time of sample collection. M. tuberculosis was identified by reference standard culture among 34/98 (35%) MDR-TB suspects. Overall MODS sensitivity for M. tuberculosis detection was 85% (95% CI, 69-95%) and specificity was 93% (95% CI, 84-98%); diagnostic accuracy did not significantly differ by HIV infection status. Median time to positivity was significantly shorter for MODS (7 days; IQR 7-15 days) than MGIT (12 days; IQR 6-16 days) or LJ (28 days; IQR 21-35 days; p<0.001). Of 33 specimens with concurrent DST results, sensitivity of the MODS assay for detection of resistance to isoniazid, rifampin, and MDR-TB was 88% (95% CI, 68-97%), 96% (95% CI, 79-100%), and 91% (95% CI, 72-99%), respectively; specificity was 89% (95% CI, 52-100%), 89% (95% CI, 52-100%), and 90% (95% CI, 56-100%), respectively.

Conclusion: In a high HIV-prevalence setting, MODS diagnosed TB and drug-resistant TB with high sensitivity and shorter turnaround time compared with standard culture and DST methods.

Show MeSH
Related in: MedlinePlus