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Flow cytometric cell sorting and in vitro pre-osteoinduction are not requirements for in vivo bone formation by human adipose-derived stromal cells.

Liu Y, Zhao Y, Zhang X, Chen T, Zhao X, Ma GE, Zhou Y - PLoS ONE (2013)

Bottom Line: We found that pre-OI enhanced the in vitro osteogenic capacity of hASCs.Interestingly, we also found that purified hASCs had an osteogenic potential similar to that of unpurified hASCs in vitro and in vivo.These results suggest that FCCS and in vitro pre-OI are not requirements for in vivo bone formation by hASCs.

View Article: PubMed Central - PubMed

Affiliation: Department of Prosthodontics, School and Hospital of Stomatology, Peking University, Beijing, China.

ABSTRACT
Human adipose-derived stromal cells (hASCs) are a promising cell source for bone tissue engineering. However, before the clinical application of hASCs for the treatment of bone defects, key questions require answers, including whether pre-osteoinduction (OI) and flow cytometric cell purification are indispensible steps for in vivo bone formation by hASCs. In this study, hASCs were purified by flow cytometric cell sorting (FCCS). The osteogenic capabilities of hASCs and purified hASCs with or without pre-osteoinduction were examined through in vitro and in vivo experiments. We found that pre-OI enhanced the in vitro osteogenic capacity of hASCs. However, 8 weeks after in vivo implantation, there were no significant differences between hASCs and hASCs that had undergone OI (hASCs+OI) or between purified hASCs and purified hASCs+OI (P>0.05). Interestingly, we also found that purified hASCs had an osteogenic potential similar to that of unpurified hASCs in vitro and in vivo. These results suggest that FCCS and in vitro pre-OI are not requirements for in vivo bone formation by hASCs.

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Related in: MedlinePlus

Growth curves for human adipose-derived stromal cells (hASCs) and purified hASCs.There were no significant differences in the proliferative capacities of the two cell types during day 1 (d1) to day 6 (d6) (P>0.05).
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pone-0056002-g003: Growth curves for human adipose-derived stromal cells (hASCs) and purified hASCs.There were no significant differences in the proliferative capacities of the two cell types during day 1 (d1) to day 6 (d6) (P>0.05).

Mentions: To compare the proliferative capacities of hASCs and purified hASCs, the two types of cell were counted daily from day 1 to day 6 (Fig. 3). These counts demonstrated no significant differences in their proliferative capacity at any time during cell proliferation (P>0.05).


Flow cytometric cell sorting and in vitro pre-osteoinduction are not requirements for in vivo bone formation by human adipose-derived stromal cells.

Liu Y, Zhao Y, Zhang X, Chen T, Zhao X, Ma GE, Zhou Y - PLoS ONE (2013)

Growth curves for human adipose-derived stromal cells (hASCs) and purified hASCs.There were no significant differences in the proliferative capacities of the two cell types during day 1 (d1) to day 6 (d6) (P>0.05).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3569421&req=5

pone-0056002-g003: Growth curves for human adipose-derived stromal cells (hASCs) and purified hASCs.There were no significant differences in the proliferative capacities of the two cell types during day 1 (d1) to day 6 (d6) (P>0.05).
Mentions: To compare the proliferative capacities of hASCs and purified hASCs, the two types of cell were counted daily from day 1 to day 6 (Fig. 3). These counts demonstrated no significant differences in their proliferative capacity at any time during cell proliferation (P>0.05).

Bottom Line: We found that pre-OI enhanced the in vitro osteogenic capacity of hASCs.Interestingly, we also found that purified hASCs had an osteogenic potential similar to that of unpurified hASCs in vitro and in vivo.These results suggest that FCCS and in vitro pre-OI are not requirements for in vivo bone formation by hASCs.

View Article: PubMed Central - PubMed

Affiliation: Department of Prosthodontics, School and Hospital of Stomatology, Peking University, Beijing, China.

ABSTRACT
Human adipose-derived stromal cells (hASCs) are a promising cell source for bone tissue engineering. However, before the clinical application of hASCs for the treatment of bone defects, key questions require answers, including whether pre-osteoinduction (OI) and flow cytometric cell purification are indispensible steps for in vivo bone formation by hASCs. In this study, hASCs were purified by flow cytometric cell sorting (FCCS). The osteogenic capabilities of hASCs and purified hASCs with or without pre-osteoinduction were examined through in vitro and in vivo experiments. We found that pre-OI enhanced the in vitro osteogenic capacity of hASCs. However, 8 weeks after in vivo implantation, there were no significant differences between hASCs and hASCs that had undergone OI (hASCs+OI) or between purified hASCs and purified hASCs+OI (P>0.05). Interestingly, we also found that purified hASCs had an osteogenic potential similar to that of unpurified hASCs in vitro and in vivo. These results suggest that FCCS and in vitro pre-OI are not requirements for in vivo bone formation by hASCs.

Show MeSH
Related in: MedlinePlus