Limits...
Flow cytometric cell sorting and in vitro pre-osteoinduction are not requirements for in vivo bone formation by human adipose-derived stromal cells.

Liu Y, Zhao Y, Zhang X, Chen T, Zhao X, Ma GE, Zhou Y - PLoS ONE (2013)

Bottom Line: We found that pre-OI enhanced the in vitro osteogenic capacity of hASCs.Interestingly, we also found that purified hASCs had an osteogenic potential similar to that of unpurified hASCs in vitro and in vivo.These results suggest that FCCS and in vitro pre-OI are not requirements for in vivo bone formation by hASCs.

View Article: PubMed Central - PubMed

Affiliation: Department of Prosthodontics, School and Hospital of Stomatology, Peking University, Beijing, China.

ABSTRACT
Human adipose-derived stromal cells (hASCs) are a promising cell source for bone tissue engineering. However, before the clinical application of hASCs for the treatment of bone defects, key questions require answers, including whether pre-osteoinduction (OI) and flow cytometric cell purification are indispensible steps for in vivo bone formation by hASCs. In this study, hASCs were purified by flow cytometric cell sorting (FCCS). The osteogenic capabilities of hASCs and purified hASCs with or without pre-osteoinduction were examined through in vitro and in vivo experiments. We found that pre-OI enhanced the in vitro osteogenic capacity of hASCs. However, 8 weeks after in vivo implantation, there were no significant differences between hASCs and hASCs that had undergone OI (hASCs+OI) or between purified hASCs and purified hASCs+OI (P>0.05). Interestingly, we also found that purified hASCs had an osteogenic potential similar to that of unpurified hASCs in vitro and in vivo. These results suggest that FCCS and in vitro pre-OI are not requirements for in vivo bone formation by hASCs.

Show MeSH
Passaged human adipose-derived stromal cells (hASCs) express mesenchymal stem cell (MSC)-specific surface markers.hASCs of the third passage (P3) expressed the MSC-specific surface markers CD44, CD73, CD90 and CD105, but did not express CD45 or HLA-DR, which are hematopoietic cell-specific markers. Isotype controls (Iso) were used in all flow cytometry experiments.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3569421&req=5

pone-0056002-g001: Passaged human adipose-derived stromal cells (hASCs) express mesenchymal stem cell (MSC)-specific surface markers.hASCs of the third passage (P3) expressed the MSC-specific surface markers CD44, CD73, CD90 and CD105, but did not express CD45 or HLA-DR, which are hematopoietic cell-specific markers. Isotype controls (Iso) were used in all flow cytometry experiments.

Mentions: hASCs were isolated from the adipose tissues of two patients. The multi-lineage potential of the hASCs was verified (data not shown) by the methods described in previous studies [3], [4], [6]. hASCs of the third passage (P3) expressed the MSC-specific surface markers CD44, CD73, CD90 and CD105, but did not express CD45 or HLA-DR (Fig. 1), which are specific markers of hematopoietic cells.


Flow cytometric cell sorting and in vitro pre-osteoinduction are not requirements for in vivo bone formation by human adipose-derived stromal cells.

Liu Y, Zhao Y, Zhang X, Chen T, Zhao X, Ma GE, Zhou Y - PLoS ONE (2013)

Passaged human adipose-derived stromal cells (hASCs) express mesenchymal stem cell (MSC)-specific surface markers.hASCs of the third passage (P3) expressed the MSC-specific surface markers CD44, CD73, CD90 and CD105, but did not express CD45 or HLA-DR, which are hematopoietic cell-specific markers. Isotype controls (Iso) were used in all flow cytometry experiments.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3569421&req=5

pone-0056002-g001: Passaged human adipose-derived stromal cells (hASCs) express mesenchymal stem cell (MSC)-specific surface markers.hASCs of the third passage (P3) expressed the MSC-specific surface markers CD44, CD73, CD90 and CD105, but did not express CD45 or HLA-DR, which are hematopoietic cell-specific markers. Isotype controls (Iso) were used in all flow cytometry experiments.
Mentions: hASCs were isolated from the adipose tissues of two patients. The multi-lineage potential of the hASCs was verified (data not shown) by the methods described in previous studies [3], [4], [6]. hASCs of the third passage (P3) expressed the MSC-specific surface markers CD44, CD73, CD90 and CD105, but did not express CD45 or HLA-DR (Fig. 1), which are specific markers of hematopoietic cells.

Bottom Line: We found that pre-OI enhanced the in vitro osteogenic capacity of hASCs.Interestingly, we also found that purified hASCs had an osteogenic potential similar to that of unpurified hASCs in vitro and in vivo.These results suggest that FCCS and in vitro pre-OI are not requirements for in vivo bone formation by hASCs.

View Article: PubMed Central - PubMed

Affiliation: Department of Prosthodontics, School and Hospital of Stomatology, Peking University, Beijing, China.

ABSTRACT
Human adipose-derived stromal cells (hASCs) are a promising cell source for bone tissue engineering. However, before the clinical application of hASCs for the treatment of bone defects, key questions require answers, including whether pre-osteoinduction (OI) and flow cytometric cell purification are indispensible steps for in vivo bone formation by hASCs. In this study, hASCs were purified by flow cytometric cell sorting (FCCS). The osteogenic capabilities of hASCs and purified hASCs with or without pre-osteoinduction were examined through in vitro and in vivo experiments. We found that pre-OI enhanced the in vitro osteogenic capacity of hASCs. However, 8 weeks after in vivo implantation, there were no significant differences between hASCs and hASCs that had undergone OI (hASCs+OI) or between purified hASCs and purified hASCs+OI (P>0.05). Interestingly, we also found that purified hASCs had an osteogenic potential similar to that of unpurified hASCs in vitro and in vivo. These results suggest that FCCS and in vitro pre-OI are not requirements for in vivo bone formation by hASCs.

Show MeSH