Limits...
Analysis of gene expression during odontogenic differentiation of cultured human dental pulp cells.

Seo MS, Hwang KG, Kim H, Baek SH - Restor Dent Endod (2012)

Bottom Line: Total RNA was extracted from cells and analyzed by microarray analysis and the key results were confirmed selectively by reverse-transcriptase polymerase chain reaction (RT-PCR).GSEA revealed that in the induced cells, genes implicated in Apoptosis and Signaling by wingless MMTV integration (Wnt) were significantly upregulated.Genes implicated in Apoptosis and Signaling by Wnt are highly connected to the differentiation of dental pulp cells into odontoblast.

View Article: PubMed Central - PubMed

Affiliation: Department of Dentistry, Hanyang University College of Medicine, Seoul, Korea. ; Department of Conservative Dentistry, Seoul National University School of Dentistry, Seoul, Korea.

ABSTRACT

Objectives: We analyzed gene-expression profiles after 14 day odontogenic induction of human dental pulp cells (DPCs) using a DNA microarray and sought candidate genes possibly associated with mineralization.

Materials and methods: Induced human dental pulp cells were obtained by culturing DPCs in odontogenic induction medium (OM) for 14 day. Cells exposed to normal culture medium were used as controls. Total RNA was extracted from cells and analyzed by microarray analysis and the key results were confirmed selectively by reverse-transcriptase polymerase chain reaction (RT-PCR). We also performed a gene set enrichment analysis (GSEA) of the microarray data.

Results: Six hundred and five genes among the 47,320 probes on the BeadChip differed by a factor of more than two-fold in the induced cells. Of these, 217 genes were upregulated, and 388 were down-regulated. GSEA revealed that in the induced cells, genes implicated in Apoptosis and Signaling by wingless MMTV integration (Wnt) were significantly upregulated.

Conclusions: Genes implicated in Apoptosis and Signaling by Wnt are highly connected to the differentiation of dental pulp cells into odontoblast.

No MeSH data available.


Related in: MedlinePlus

Gene set enrichment analysis (GSEA) identifying novel pathways activated in odontogenic inducted cells relative to the control cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3569398&req=5

Figure 2: Gene set enrichment analysis (GSEA) identifying novel pathways activated in odontogenic inducted cells relative to the control cells.

Mentions: GSEA revealed that odontogenic induction significantly upregulated genes implicated in Apoptosis and Signaling by wingless MMTV integration (Wnt) (p < 0.01) (Figure 2).


Analysis of gene expression during odontogenic differentiation of cultured human dental pulp cells.

Seo MS, Hwang KG, Kim H, Baek SH - Restor Dent Endod (2012)

Gene set enrichment analysis (GSEA) identifying novel pathways activated in odontogenic inducted cells relative to the control cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3569398&req=5

Figure 2: Gene set enrichment analysis (GSEA) identifying novel pathways activated in odontogenic inducted cells relative to the control cells.
Mentions: GSEA revealed that odontogenic induction significantly upregulated genes implicated in Apoptosis and Signaling by wingless MMTV integration (Wnt) (p < 0.01) (Figure 2).

Bottom Line: Total RNA was extracted from cells and analyzed by microarray analysis and the key results were confirmed selectively by reverse-transcriptase polymerase chain reaction (RT-PCR).GSEA revealed that in the induced cells, genes implicated in Apoptosis and Signaling by wingless MMTV integration (Wnt) were significantly upregulated.Genes implicated in Apoptosis and Signaling by Wnt are highly connected to the differentiation of dental pulp cells into odontoblast.

View Article: PubMed Central - PubMed

Affiliation: Department of Dentistry, Hanyang University College of Medicine, Seoul, Korea. ; Department of Conservative Dentistry, Seoul National University School of Dentistry, Seoul, Korea.

ABSTRACT

Objectives: We analyzed gene-expression profiles after 14 day odontogenic induction of human dental pulp cells (DPCs) using a DNA microarray and sought candidate genes possibly associated with mineralization.

Materials and methods: Induced human dental pulp cells were obtained by culturing DPCs in odontogenic induction medium (OM) for 14 day. Cells exposed to normal culture medium were used as controls. Total RNA was extracted from cells and analyzed by microarray analysis and the key results were confirmed selectively by reverse-transcriptase polymerase chain reaction (RT-PCR). We also performed a gene set enrichment analysis (GSEA) of the microarray data.

Results: Six hundred and five genes among the 47,320 probes on the BeadChip differed by a factor of more than two-fold in the induced cells. Of these, 217 genes were upregulated, and 388 were down-regulated. GSEA revealed that in the induced cells, genes implicated in Apoptosis and Signaling by wingless MMTV integration (Wnt) were significantly upregulated.

Conclusions: Genes implicated in Apoptosis and Signaling by Wnt are highly connected to the differentiation of dental pulp cells into odontoblast.

No MeSH data available.


Related in: MedlinePlus