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Complete genome sequence of the motile actinomycete Actinoplanes missouriensis 431(T) (= NBRC 102363(T)).

Yamamura H, Ohnishi Y, Ishikawa J, Ichikawa N, Ikeda H, Sekine M, Harada T, Horinouchi S, Otoguro M, Tamura T, Suzuki K, Hoshino Y, Arisawa A, Nakagawa Y, Fujita N, Hayakawa M - Stand Genomic Sci (2012)

Bottom Line: It is of further interest that members of Actinoplanes are prolific sources of novel antibiotics, enzymes, and other bioactive compounds.Here, we describe the features of A. missouriensis 431(T), together with the complete genome sequence and annotation.The 8,773,466 bp genome contains 8,125 protein-coding and 79 RNA genes.

View Article: PubMed Central - PubMed

Affiliation: Division of Applied Biological Sciences, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Yamanashi, Japan.

ABSTRACT
Actinoplanes missouriensis Couch 1963 is a well-characterized member of the genus Actinoplanes, which is of morphological interest because its members typically produce sporangia containing motile spores. The sporangiospores are motile by means of flagella and exhibit chemotactic properties. It is of further interest that members of Actinoplanes are prolific sources of novel antibiotics, enzymes, and other bioactive compounds. Here, we describe the features of A. missouriensis 431(T), together with the complete genome sequence and annotation. The 8,773,466 bp genome contains 8,125 protein-coding and 79 RNA genes.

No MeSH data available.


Related in: MedlinePlus

Graphical circular map of the strain 431T genome. From outside to the center: genes on forward strand (color by COG categories), Genes on reverse strand (color by COG categories), RNA genes (tRNA genes, green; rRNA genes, red; other RNA gene types, black), GC content, GC skew.
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f3: Graphical circular map of the strain 431T genome. From outside to the center: genes on forward strand (color by COG categories), Genes on reverse strand (color by COG categories), RNA genes (tRNA genes, green; rRNA genes, red; other RNA gene types, black), GC content, GC skew.

Mentions: The genome of strain 431T consists of one circular chromosome with a length of 8,773,466 bp and a G+C content of 70.8% ( Table 3 and Figure 3). A. missouriensis DSM 43046T was reported to contain a linear plasmid, pAM1 [33]; however, the corresponding plasmid was not found in strain 431T (= NBRC 102363T) by CHEF electrophoresis analysis. It is possible that the linear plasmid was cured from strain 431T by repeated subculturing in the laboratory. In comparison to other members of the family Micromonosporaceae, such as Salinispora and Micromonospora, A. missouriensis has a larger genome size. Of the total of 8,204 predicted genes, 8,125 were protein-coding genes and 79 were RNA genes. More than half of the protein-coding genes (4,539, 55.9%) were assigned a putative function, while the remaining predicted genes were annotated as hypothetical proteins. The distribution of genes into COGs functional categories is presented in Table 4.


Complete genome sequence of the motile actinomycete Actinoplanes missouriensis 431(T) (= NBRC 102363(T)).

Yamamura H, Ohnishi Y, Ishikawa J, Ichikawa N, Ikeda H, Sekine M, Harada T, Horinouchi S, Otoguro M, Tamura T, Suzuki K, Hoshino Y, Arisawa A, Nakagawa Y, Fujita N, Hayakawa M - Stand Genomic Sci (2012)

Graphical circular map of the strain 431T genome. From outside to the center: genes on forward strand (color by COG categories), Genes on reverse strand (color by COG categories), RNA genes (tRNA genes, green; rRNA genes, red; other RNA gene types, black), GC content, GC skew.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3569393&req=5

f3: Graphical circular map of the strain 431T genome. From outside to the center: genes on forward strand (color by COG categories), Genes on reverse strand (color by COG categories), RNA genes (tRNA genes, green; rRNA genes, red; other RNA gene types, black), GC content, GC skew.
Mentions: The genome of strain 431T consists of one circular chromosome with a length of 8,773,466 bp and a G+C content of 70.8% ( Table 3 and Figure 3). A. missouriensis DSM 43046T was reported to contain a linear plasmid, pAM1 [33]; however, the corresponding plasmid was not found in strain 431T (= NBRC 102363T) by CHEF electrophoresis analysis. It is possible that the linear plasmid was cured from strain 431T by repeated subculturing in the laboratory. In comparison to other members of the family Micromonosporaceae, such as Salinispora and Micromonospora, A. missouriensis has a larger genome size. Of the total of 8,204 predicted genes, 8,125 were protein-coding genes and 79 were RNA genes. More than half of the protein-coding genes (4,539, 55.9%) were assigned a putative function, while the remaining predicted genes were annotated as hypothetical proteins. The distribution of genes into COGs functional categories is presented in Table 4.

Bottom Line: It is of further interest that members of Actinoplanes are prolific sources of novel antibiotics, enzymes, and other bioactive compounds.Here, we describe the features of A. missouriensis 431(T), together with the complete genome sequence and annotation.The 8,773,466 bp genome contains 8,125 protein-coding and 79 RNA genes.

View Article: PubMed Central - PubMed

Affiliation: Division of Applied Biological Sciences, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Yamanashi, Japan.

ABSTRACT
Actinoplanes missouriensis Couch 1963 is a well-characterized member of the genus Actinoplanes, which is of morphological interest because its members typically produce sporangia containing motile spores. The sporangiospores are motile by means of flagella and exhibit chemotactic properties. It is of further interest that members of Actinoplanes are prolific sources of novel antibiotics, enzymes, and other bioactive compounds. Here, we describe the features of A. missouriensis 431(T), together with the complete genome sequence and annotation. The 8,773,466 bp genome contains 8,125 protein-coding and 79 RNA genes.

No MeSH data available.


Related in: MedlinePlus