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Non-contiguous finished genome sequence and description of Herbaspirillum massiliense sp. nov.

Lagier JC, Gimenez G, Robert C, Raoult D, Fournier PE - Stand Genomic Sci (2012)

Bottom Line: H. massiliense is an aerobic rod.Here we describe the features of this organism, together with the complete genome sequence and annotation.The 4,186,486 bp long genome (one chromosome but no plasmid) contains 3,847 protein-coding and 54 RNA genes, including 3 rRNA genes.

View Article: PubMed Central - PubMed

Affiliation: Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, Aix-Marseille Université

ABSTRACT
Herbaspirillum massiliense strain JC206(T) sp. nov. is the type strain of H. massiliense sp. nov., a new species within the genus Herbaspirillum. This strain, whose genome is described here, was isolated from the fecal flora of a healthy Senegalese patient. H. massiliense is an aerobic rod. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 4,186,486 bp long genome (one chromosome but no plasmid) contains 3,847 protein-coding and 54 RNA genes, including 3 rRNA genes.

No MeSH data available.


Related in: MedlinePlus

Transmission electron microscopy of H. massiliense strain JC206T, using a Morgani 268D (Philips) at an operating voltage of 60kV.The scale bar represents 900 nm.
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f3: Transmission electron microscopy of H. massiliense strain JC206T, using a Morgani 268D (Philips) at an operating voltage of 60kV.The scale bar represents 900 nm.

Mentions: Different growth temperatures (25, 30, 37, 45°C) were tested. No growth occurred at either 25°C or 45°C, growth occurred at either 30 or 37°C. Optimal growth was observed at 37°C. Colonies were light brown, opaque and 0.5 mm in diameter on blood-enriched Columbia agar and Brain Heart Infusion (BHI) agar. Growth of the strain was tested under anaerobic and microaerophilic conditions using GENbag anaer and GENbag microaer systems, respectively (BioMérieux), and in the presence of air, of 5% CO2 and in anaerobic conditions. Optimal growth was obtained aerobically, with weak growth being observed under microaerophilic condition and with 5% CO2. No growth occurred under anaerobic conditions. Gram staining showed Gram negative curved rods (Figure 2). A motility test was positive. Cells grown on agar have a mean diameter of 0.44 µm by electron microscopy and have several polar flagella (Figure 3).


Non-contiguous finished genome sequence and description of Herbaspirillum massiliense sp. nov.

Lagier JC, Gimenez G, Robert C, Raoult D, Fournier PE - Stand Genomic Sci (2012)

Transmission electron microscopy of H. massiliense strain JC206T, using a Morgani 268D (Philips) at an operating voltage of 60kV.The scale bar represents 900 nm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3569391&req=5

f3: Transmission electron microscopy of H. massiliense strain JC206T, using a Morgani 268D (Philips) at an operating voltage of 60kV.The scale bar represents 900 nm.
Mentions: Different growth temperatures (25, 30, 37, 45°C) were tested. No growth occurred at either 25°C or 45°C, growth occurred at either 30 or 37°C. Optimal growth was observed at 37°C. Colonies were light brown, opaque and 0.5 mm in diameter on blood-enriched Columbia agar and Brain Heart Infusion (BHI) agar. Growth of the strain was tested under anaerobic and microaerophilic conditions using GENbag anaer and GENbag microaer systems, respectively (BioMérieux), and in the presence of air, of 5% CO2 and in anaerobic conditions. Optimal growth was obtained aerobically, with weak growth being observed under microaerophilic condition and with 5% CO2. No growth occurred under anaerobic conditions. Gram staining showed Gram negative curved rods (Figure 2). A motility test was positive. Cells grown on agar have a mean diameter of 0.44 µm by electron microscopy and have several polar flagella (Figure 3).

Bottom Line: H. massiliense is an aerobic rod.Here we describe the features of this organism, together with the complete genome sequence and annotation.The 4,186,486 bp long genome (one chromosome but no plasmid) contains 3,847 protein-coding and 54 RNA genes, including 3 rRNA genes.

View Article: PubMed Central - PubMed

Affiliation: Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, Aix-Marseille Université

ABSTRACT
Herbaspirillum massiliense strain JC206(T) sp. nov. is the type strain of H. massiliense sp. nov., a new species within the genus Herbaspirillum. This strain, whose genome is described here, was isolated from the fecal flora of a healthy Senegalese patient. H. massiliense is an aerobic rod. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 4,186,486 bp long genome (one chromosome but no plasmid) contains 3,847 protein-coding and 54 RNA genes, including 3 rRNA genes.

No MeSH data available.


Related in: MedlinePlus