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Complete genome sequence of Corynebacterium pseudotuberculosis biovar ovis strain P54B96 isolated from antelope in South Africa obtained by rapid next generation sequencing technology.

Hassan SS, Guimarães LC, Pereira Ude P, Islam A, Ali A, Bakhtiar SM, Ribeiro D, Rodrigues Dos Santos A, Soares Sde C, Dorella F, Pinto AC, Schneider MP, Barbosa MS, Almeida S, Abreu V, Aburjaile F, Carneiro AR, Cerdeira LT, Fiaux K, Barbosa E, Diniz C, Rocha FS, Ramos RT, Jain N, Tiwari S, Barh D, Miyoshi A, Müller B, Silva A, Azevedo V - Stand Genomic Sci (2012)

Bottom Line: This strain is interesting in the sense that it has been found together with non-tuberculous mycobacteria (NTMs) which could nevertheless play a role in the lesion formation.In this work, we describe a set of features of C. pseudotuberculosis P54B96, together with the details of the complete genome sequence and annotation.The genome comprises of 2.34 Mbp long, single circular genome with 2,084 protein-coding genes, 12 rRNA, 49 tRNA and 62 pseudogenes and a G+C content of 52.19%.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Genética Celular e Molecular, Departamento de Biologia Geral, Instituto de Ciências Biológicas (ICB), Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.

ABSTRACT
The Actinobacteria, Corynebacterium pseudotuberculosis strain P54B96, a nonmotile, non-sporulating and a mesophile bacterium, was isolated from liver, lung and mediastinal lymph node lesions in an antelope from South Africa. This strain is interesting in the sense that it has been found together with non-tuberculous mycobacteria (NTMs) which could nevertheless play a role in the lesion formation. In this work, we describe a set of features of C. pseudotuberculosis P54B96, together with the details of the complete genome sequence and annotation. The genome comprises of 2.34 Mbp long, single circular genome with 2,084 protein-coding genes, 12 rRNA, 49 tRNA and 62 pseudogenes and a G+C content of 52.19%. The analysis of the genome sequence provides means to better understanding the molecular and genetic basis of virulence of this bacterium, enabling a detailed investigation of its pathogenesis.

No MeSH data available.


Related in: MedlinePlus

Graphical circular map of the genome. From outside to the center: Genes on forward strand (color by COG categories), Genes on reverse strand (color by COG categories), RNA genes (tRNAs green, rRNAs red, other RNAs black), GC content, GC skew.
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f2: Graphical circular map of the genome. From outside to the center: Genes on forward strand (color by COG categories), Genes on reverse strand (color by COG categories), RNA genes (tRNAs green, rRNAs red, other RNAs black), GC content, GC skew.

Mentions: The genome is 2,337,657 bp long and comprises one main circular chromosome with a 52.19% GC content. A total of 2,207 genes were predicted, among which 2,146 were protein coding genes, and 61 RNAs; 62 pseudogenes were also identified. Of the whole genome, 69.01% comprise genes that were assigned with putative functions, while the remaining genes were annotated as hypothetical proteins. The properties and statistics of the C. pseudotuberculosis genome are listed in Table 3. The distributions of genes into COGs functional categories is presented in Figure 2 and Table 4, followed by a cellular overview diagram in Figure 3 and a summary of metabolic network statistics shown in Table 5.


Complete genome sequence of Corynebacterium pseudotuberculosis biovar ovis strain P54B96 isolated from antelope in South Africa obtained by rapid next generation sequencing technology.

Hassan SS, Guimarães LC, Pereira Ude P, Islam A, Ali A, Bakhtiar SM, Ribeiro D, Rodrigues Dos Santos A, Soares Sde C, Dorella F, Pinto AC, Schneider MP, Barbosa MS, Almeida S, Abreu V, Aburjaile F, Carneiro AR, Cerdeira LT, Fiaux K, Barbosa E, Diniz C, Rocha FS, Ramos RT, Jain N, Tiwari S, Barh D, Miyoshi A, Müller B, Silva A, Azevedo V - Stand Genomic Sci (2012)

Graphical circular map of the genome. From outside to the center: Genes on forward strand (color by COG categories), Genes on reverse strand (color by COG categories), RNA genes (tRNAs green, rRNAs red, other RNAs black), GC content, GC skew.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3569390&req=5

f2: Graphical circular map of the genome. From outside to the center: Genes on forward strand (color by COG categories), Genes on reverse strand (color by COG categories), RNA genes (tRNAs green, rRNAs red, other RNAs black), GC content, GC skew.
Mentions: The genome is 2,337,657 bp long and comprises one main circular chromosome with a 52.19% GC content. A total of 2,207 genes were predicted, among which 2,146 were protein coding genes, and 61 RNAs; 62 pseudogenes were also identified. Of the whole genome, 69.01% comprise genes that were assigned with putative functions, while the remaining genes were annotated as hypothetical proteins. The properties and statistics of the C. pseudotuberculosis genome are listed in Table 3. The distributions of genes into COGs functional categories is presented in Figure 2 and Table 4, followed by a cellular overview diagram in Figure 3 and a summary of metabolic network statistics shown in Table 5.

Bottom Line: This strain is interesting in the sense that it has been found together with non-tuberculous mycobacteria (NTMs) which could nevertheless play a role in the lesion formation.In this work, we describe a set of features of C. pseudotuberculosis P54B96, together with the details of the complete genome sequence and annotation.The genome comprises of 2.34 Mbp long, single circular genome with 2,084 protein-coding genes, 12 rRNA, 49 tRNA and 62 pseudogenes and a G+C content of 52.19%.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Genética Celular e Molecular, Departamento de Biologia Geral, Instituto de Ciências Biológicas (ICB), Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.

ABSTRACT
The Actinobacteria, Corynebacterium pseudotuberculosis strain P54B96, a nonmotile, non-sporulating and a mesophile bacterium, was isolated from liver, lung and mediastinal lymph node lesions in an antelope from South Africa. This strain is interesting in the sense that it has been found together with non-tuberculous mycobacteria (NTMs) which could nevertheless play a role in the lesion formation. In this work, we describe a set of features of C. pseudotuberculosis P54B96, together with the details of the complete genome sequence and annotation. The genome comprises of 2.34 Mbp long, single circular genome with 2,084 protein-coding genes, 12 rRNA, 49 tRNA and 62 pseudogenes and a G+C content of 52.19%. The analysis of the genome sequence provides means to better understanding the molecular and genetic basis of virulence of this bacterium, enabling a detailed investigation of its pathogenesis.

No MeSH data available.


Related in: MedlinePlus