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Non-contiguous finished genome sequence and description of Brevibacterium senegalense sp. nov.

Kokcha S, Ramasamy D, Lagier JC, Robert C, Raoult D, Fournier PE - Stand Genomic Sci (2012)

Bottom Line: This strain, whose genome is described here, was isolated from the fecal flora of a healthy Senegalese patient.Here we describe the features of this organism, together with the complete genome sequence and annotation.The 3,425,960 bp long genome (1 chromosome but no plasmid) contains 3,064 protein-coding and 49 RNA genes.

View Article: PubMed Central - PubMed

Affiliation: Aix-Marseille Université, Faculté de médecine.

ABSTRACT
Brevibacterium senegalense strain JC43(T) sp. nov. is the type strain of Brevibacterium senegalense sp. nov., a new species within the Brevibacterium genus. This strain, whose genome is described here, was isolated from the fecal flora of a healthy Senegalese patient. B. senegalense is an aerobic rod-shaped Gram-positive bacterium. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 3,425,960 bp long genome (1 chromosome but no plasmid) contains 3,064 protein-coding and 49 RNA genes.

No MeSH data available.


Related in: MedlinePlus

Reference mass spectrum from B. senegalense strain JC43T. Spectra from 4 individual colonies were compared and a reference spectrum was generated.
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f4: Reference mass spectrum from B. senegalense strain JC43T. Spectra from 4 individual colonies were compared and a reference spectrum was generated.

Mentions: Matrix-assisted laser-desorption/ionization time-of-flight (MALDI-TOF) MS protein analysis was carried out as previously described [5,34] using a Microflex spectrometer (Bruker Daltonics, Germany). Twelve distinct deposits were done for strain JC43T from four isolated colonies. The 12 JC43T spectra were imported into the MALDI BioTyper software (version 2.0, Bruker) and analyzed by standard pattern matching (with default parameter settings) against the main spectra of 3,769 bacteria, which were used as reference data, in the BioTyper database. The database contained 41 spectra from 18 validly published Brevibacterium species, including B. avium,B. celere, B. casei, B. aurantiacum, B. epidermidis, B. iodinum, B. linens, B. luteolum, B. marinum, B. massiliense, B. mcbrellneri, B. otitidis, B. paucivorans, B. picturae, B. pityocampae, B. ravenspurgense, B. sanguinis and B. stationis. No significant score was obtained for strain JC43, thus suggesting that our isolate was not a member of a known Brevibacterium species within the Bruker database. We incremented our database with the spectrum from strain JC43 (Figure 4).


Non-contiguous finished genome sequence and description of Brevibacterium senegalense sp. nov.

Kokcha S, Ramasamy D, Lagier JC, Robert C, Raoult D, Fournier PE - Stand Genomic Sci (2012)

Reference mass spectrum from B. senegalense strain JC43T. Spectra from 4 individual colonies were compared and a reference spectrum was generated.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3569389&req=5

f4: Reference mass spectrum from B. senegalense strain JC43T. Spectra from 4 individual colonies were compared and a reference spectrum was generated.
Mentions: Matrix-assisted laser-desorption/ionization time-of-flight (MALDI-TOF) MS protein analysis was carried out as previously described [5,34] using a Microflex spectrometer (Bruker Daltonics, Germany). Twelve distinct deposits were done for strain JC43T from four isolated colonies. The 12 JC43T spectra were imported into the MALDI BioTyper software (version 2.0, Bruker) and analyzed by standard pattern matching (with default parameter settings) against the main spectra of 3,769 bacteria, which were used as reference data, in the BioTyper database. The database contained 41 spectra from 18 validly published Brevibacterium species, including B. avium,B. celere, B. casei, B. aurantiacum, B. epidermidis, B. iodinum, B. linens, B. luteolum, B. marinum, B. massiliense, B. mcbrellneri, B. otitidis, B. paucivorans, B. picturae, B. pityocampae, B. ravenspurgense, B. sanguinis and B. stationis. No significant score was obtained for strain JC43, thus suggesting that our isolate was not a member of a known Brevibacterium species within the Bruker database. We incremented our database with the spectrum from strain JC43 (Figure 4).

Bottom Line: This strain, whose genome is described here, was isolated from the fecal flora of a healthy Senegalese patient.Here we describe the features of this organism, together with the complete genome sequence and annotation.The 3,425,960 bp long genome (1 chromosome but no plasmid) contains 3,064 protein-coding and 49 RNA genes.

View Article: PubMed Central - PubMed

Affiliation: Aix-Marseille Université, Faculté de médecine.

ABSTRACT
Brevibacterium senegalense strain JC43(T) sp. nov. is the type strain of Brevibacterium senegalense sp. nov., a new species within the Brevibacterium genus. This strain, whose genome is described here, was isolated from the fecal flora of a healthy Senegalese patient. B. senegalense is an aerobic rod-shaped Gram-positive bacterium. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 3,425,960 bp long genome (1 chromosome but no plasmid) contains 3,064 protein-coding and 49 RNA genes.

No MeSH data available.


Related in: MedlinePlus