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Non contiguous-finished genome sequence and description of Cellulomonas massiliensis sp. nov.

Lagier JC, Ramasamy D, Rivet R, Raoult D, Fournier PE - Stand Genomic Sci (2012)

Bottom Line: This strain, whose genome is described here, was isolated from the fecal flora of a healthy Senegalese patient.Here we describe the features of this organism, together with the complete genome sequence and annotation.The 3,407,283 bp long genome contains 3,083 protein-coding and 48 RNA genes.

View Article: PubMed Central - PubMed

Affiliation: Aix-Marseille Université, Faculté de médecine, Marseille, France.

ABSTRACT
Cellulomonas massiliensis strain JC225(T) sp. nov. is the type strain of Cellulomonas massiliensis sp., a new species within the genus Cellulomonas. This strain, whose genome is described here, was isolated from the fecal flora of a healthy Senegalese patient. C. massiliensis is an aerobic rod-shaped bacterium. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 3,407,283 bp long genome contains 3,083 protein-coding and 48 RNA genes.

No MeSH data available.


Related in: MedlinePlus

Reference mass spectrum from C. massiliensis strain JC225T. Spectra from 12 individual colonies were compared and a reference spectrum was generated.
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f4: Reference mass spectrum from C. massiliensis strain JC225T. Spectra from 12 individual colonies were compared and a reference spectrum was generated.

Mentions: Matrix-assisted laser-desorption/ionization time-of-flight (MALDI-TOF) MS protein analysis was carried out as previously described [5,41] using a Microflex spectrometer (Bruker Daltonics, Germany). Twelve distinct deposits were done for strain JC225 from 12 isolated colonies. The 12 JC225 spectra were imported into the MALDI BioTyper software (version 2.0, Bruker) and analyzed by standard pattern matching (with default parameter settings) against the main spectra of 3,769 bacteria, which were used as reference data in the BioTyper database. The database contained 11 spectra from 8 validly published Cellulomonas species, including Cellulomonas composti, the phylogenetically closest species to C. massiliensis. No significant score was obtained for strain JC225T, thus suggesting that our isolate was not a member of a known species within the Bruker database. We incremented our database with the reference spectrum from strain JC225T (Figure 4).


Non contiguous-finished genome sequence and description of Cellulomonas massiliensis sp. nov.

Lagier JC, Ramasamy D, Rivet R, Raoult D, Fournier PE - Stand Genomic Sci (2012)

Reference mass spectrum from C. massiliensis strain JC225T. Spectra from 12 individual colonies were compared and a reference spectrum was generated.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3569388&req=5

f4: Reference mass spectrum from C. massiliensis strain JC225T. Spectra from 12 individual colonies were compared and a reference spectrum was generated.
Mentions: Matrix-assisted laser-desorption/ionization time-of-flight (MALDI-TOF) MS protein analysis was carried out as previously described [5,41] using a Microflex spectrometer (Bruker Daltonics, Germany). Twelve distinct deposits were done for strain JC225 from 12 isolated colonies. The 12 JC225 spectra were imported into the MALDI BioTyper software (version 2.0, Bruker) and analyzed by standard pattern matching (with default parameter settings) against the main spectra of 3,769 bacteria, which were used as reference data in the BioTyper database. The database contained 11 spectra from 8 validly published Cellulomonas species, including Cellulomonas composti, the phylogenetically closest species to C. massiliensis. No significant score was obtained for strain JC225T, thus suggesting that our isolate was not a member of a known species within the Bruker database. We incremented our database with the reference spectrum from strain JC225T (Figure 4).

Bottom Line: This strain, whose genome is described here, was isolated from the fecal flora of a healthy Senegalese patient.Here we describe the features of this organism, together with the complete genome sequence and annotation.The 3,407,283 bp long genome contains 3,083 protein-coding and 48 RNA genes.

View Article: PubMed Central - PubMed

Affiliation: Aix-Marseille Université, Faculté de médecine, Marseille, France.

ABSTRACT
Cellulomonas massiliensis strain JC225(T) sp. nov. is the type strain of Cellulomonas massiliensis sp., a new species within the genus Cellulomonas. This strain, whose genome is described here, was isolated from the fecal flora of a healthy Senegalese patient. C. massiliensis is an aerobic rod-shaped bacterium. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 3,407,283 bp long genome contains 3,083 protein-coding and 48 RNA genes.

No MeSH data available.


Related in: MedlinePlus