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Complete genome sequence of Liberibacter crescens BT-1.

Leonard MT, Fagen JR, Davis-Richardson AG, Davis MJ, Triplett EW - Stand Genomic Sci (2012)

Bottom Line: Comparison to other species in the Liberibacter genus, L. crescens has many more genes in thiamine and essential amino acid biosynthesis.This likely explains why L. crescens BT-1 is culturable while the known Liberibacter strains have not yet been cultured.Similar to CandidatusL. asiaticus psy62, the L. crescens BT-1 genome contains two prophage regions.

View Article: PubMed Central - PubMed

Affiliation: Microbiology and Cell Science Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, USA.

ABSTRACT
Liberibacter crescens BT-1, a Gram-negative, rod-shaped bacterial isolate, was previously recovered from mountain papaya to gain insight on Huanglongbing (HLB) and Zebra Chip (ZC) diseases. The genome of BT-1 was sequenced at the Interdisciplinary Center for Biotechnology Research (ICBR) at the University of Florida. A finished assembly and annotation yielded one chromosome with a length of 1,504,659 bp and a G+C content of 35.4%. Comparison to other species in the Liberibacter genus, L. crescens has many more genes in thiamine and essential amino acid biosynthesis. This likely explains why L. crescens BT-1 is culturable while the known Liberibacter strains have not yet been cultured. Similar to CandidatusL. asiaticus psy62, the L. crescens BT-1 genome contains two prophage regions.

No MeSH data available.


Circular genomic map of L. crescens BT-1. From outside to the center: Genes on forward strand (colored by labeled COG categories), genes on reverse strand (colored by labeled COG categories), RNA genes (tRNA green, rRNA red), putative prophage regions, GC content, GC skew.
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f3: Circular genomic map of L. crescens BT-1. From outside to the center: Genes on forward strand (colored by labeled COG categories), genes on reverse strand (colored by labeled COG categories), RNA genes (tRNA green, rRNA red), putative prophage regions, GC content, GC skew.

Mentions: From the above criteria, the locations and boundaries of two prophages in L. crescens were predicted to extend from base pair 523,789-564,039 in prophage LC1 and from base pair 848,435-886,798 in prophage LC2. Unlike the two prophages in CandidatusL. asiaticus, the prophages in L. crescens were not homologues, sharing only short (<1,000 bp) regions of moderate similarity, determined through Wise2 alignment [52]. Additionally, the prophages in L. crescens were not found in CandidatusL. asiaticus. Homology was inferred through alignment by the progressiveMauve algorithm [53] (Figures 3-5). While the SC1 phage in CandidatusL. asiaticus is known to enter a lytic cycle in the phloem of citrus, the lifecycles of the prophages in L. crescens have yet to be explored experimentally [10].


Complete genome sequence of Liberibacter crescens BT-1.

Leonard MT, Fagen JR, Davis-Richardson AG, Davis MJ, Triplett EW - Stand Genomic Sci (2012)

Circular genomic map of L. crescens BT-1. From outside to the center: Genes on forward strand (colored by labeled COG categories), genes on reverse strand (colored by labeled COG categories), RNA genes (tRNA green, rRNA red), putative prophage regions, GC content, GC skew.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3569387&req=5

f3: Circular genomic map of L. crescens BT-1. From outside to the center: Genes on forward strand (colored by labeled COG categories), genes on reverse strand (colored by labeled COG categories), RNA genes (tRNA green, rRNA red), putative prophage regions, GC content, GC skew.
Mentions: From the above criteria, the locations and boundaries of two prophages in L. crescens were predicted to extend from base pair 523,789-564,039 in prophage LC1 and from base pair 848,435-886,798 in prophage LC2. Unlike the two prophages in CandidatusL. asiaticus, the prophages in L. crescens were not homologues, sharing only short (<1,000 bp) regions of moderate similarity, determined through Wise2 alignment [52]. Additionally, the prophages in L. crescens were not found in CandidatusL. asiaticus. Homology was inferred through alignment by the progressiveMauve algorithm [53] (Figures 3-5). While the SC1 phage in CandidatusL. asiaticus is known to enter a lytic cycle in the phloem of citrus, the lifecycles of the prophages in L. crescens have yet to be explored experimentally [10].

Bottom Line: Comparison to other species in the Liberibacter genus, L. crescens has many more genes in thiamine and essential amino acid biosynthesis.This likely explains why L. crescens BT-1 is culturable while the known Liberibacter strains have not yet been cultured.Similar to CandidatusL. asiaticus psy62, the L. crescens BT-1 genome contains two prophage regions.

View Article: PubMed Central - PubMed

Affiliation: Microbiology and Cell Science Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, USA.

ABSTRACT
Liberibacter crescens BT-1, a Gram-negative, rod-shaped bacterial isolate, was previously recovered from mountain papaya to gain insight on Huanglongbing (HLB) and Zebra Chip (ZC) diseases. The genome of BT-1 was sequenced at the Interdisciplinary Center for Biotechnology Research (ICBR) at the University of Florida. A finished assembly and annotation yielded one chromosome with a length of 1,504,659 bp and a G+C content of 35.4%. Comparison to other species in the Liberibacter genus, L. crescens has many more genes in thiamine and essential amino acid biosynthesis. This likely explains why L. crescens BT-1 is culturable while the known Liberibacter strains have not yet been cultured. Similar to CandidatusL. asiaticus psy62, the L. crescens BT-1 genome contains two prophage regions.

No MeSH data available.