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Genome sequence and description of Aeromicrobium massiliense sp. nov.

Ramasamy D, Kokcha S, Lagier JC, Nguyen TT, Raoult D, Fournier PE - Stand Genomic Sci (2012)

Bottom Line: Aeromicrobium massiliense is an aerobic rod-shaped gram-positive bacterium.Here we describe the features of this organism, together with the complete genome sequence and annotation.The 3,322,119 bp long genome contains 3,296 protein-coding and 51 RNA genes.

View Article: PubMed Central - PubMed

Affiliation: Aix-Marseille Université, Faculté de médecine, Marseille, France.

ABSTRACT
Aeromicrobium massiliense strain JC14(T)sp. nov. is the type strain of Aeromicrobium massiliense sp. nov., a new species within the genus Aeromicrobium. This strain, whose genome is described here, was isolated from the fecal microbiota of an asymptomatic patient. Aeromicrobium massiliense is an aerobic rod-shaped gram-positive bacterium. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 3,322,119 bp long genome contains 3,296 protein-coding and 51 RNA genes.

No MeSH data available.


Related in: MedlinePlus

Reference mass spectrum from A. massiliense strain JC14T. Spectra from 12 individual colonies were compared and a reference spectrum was generated.
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f4: Reference mass spectrum from A. massiliense strain JC14T. Spectra from 12 individual colonies were compared and a reference spectrum was generated.

Mentions: Matrix-assisted laser-desorption/ionization time-of-flight (MALDI-TOF) MS protein analysis was carried out as previously described [7,33] using a Microflex spectrometer (Bruker Daltonics, Germany). Twelve distinct deposits were done for strain JC14T from 12 isolated colonies. The twelve JC14T spectra were imported into the MALDI BioTyper software (version 2.0, Bruker) and analyzed by standard pattern matching (with default parameter settings) against the main spectra of 3,769 bacteria, which were used as reference data, in the BioTyper database. The database contained no spectra from validly published Aeromicrobium species. No significant score was obtained for strain JC14T, thus suggesting that our isolate was not a member of a known species within the Bruker database. However, we acknowledge that the absence of other Aeromicrobium spectra does not as yet make using MALDI TOF MS a discriminatory identification criterion for A. massiliense. We incremented our database with the spectrum from strain JC14T (Figure 4).


Genome sequence and description of Aeromicrobium massiliense sp. nov.

Ramasamy D, Kokcha S, Lagier JC, Nguyen TT, Raoult D, Fournier PE - Stand Genomic Sci (2012)

Reference mass spectrum from A. massiliense strain JC14T. Spectra from 12 individual colonies were compared and a reference spectrum was generated.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3569385&req=5

f4: Reference mass spectrum from A. massiliense strain JC14T. Spectra from 12 individual colonies were compared and a reference spectrum was generated.
Mentions: Matrix-assisted laser-desorption/ionization time-of-flight (MALDI-TOF) MS protein analysis was carried out as previously described [7,33] using a Microflex spectrometer (Bruker Daltonics, Germany). Twelve distinct deposits were done for strain JC14T from 12 isolated colonies. The twelve JC14T spectra were imported into the MALDI BioTyper software (version 2.0, Bruker) and analyzed by standard pattern matching (with default parameter settings) against the main spectra of 3,769 bacteria, which were used as reference data, in the BioTyper database. The database contained no spectra from validly published Aeromicrobium species. No significant score was obtained for strain JC14T, thus suggesting that our isolate was not a member of a known species within the Bruker database. However, we acknowledge that the absence of other Aeromicrobium spectra does not as yet make using MALDI TOF MS a discriminatory identification criterion for A. massiliense. We incremented our database with the spectrum from strain JC14T (Figure 4).

Bottom Line: Aeromicrobium massiliense is an aerobic rod-shaped gram-positive bacterium.Here we describe the features of this organism, together with the complete genome sequence and annotation.The 3,322,119 bp long genome contains 3,296 protein-coding and 51 RNA genes.

View Article: PubMed Central - PubMed

Affiliation: Aix-Marseille Université, Faculté de médecine, Marseille, France.

ABSTRACT
Aeromicrobium massiliense strain JC14(T)sp. nov. is the type strain of Aeromicrobium massiliense sp. nov., a new species within the genus Aeromicrobium. This strain, whose genome is described here, was isolated from the fecal microbiota of an asymptomatic patient. Aeromicrobium massiliense is an aerobic rod-shaped gram-positive bacterium. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 3,322,119 bp long genome contains 3,296 protein-coding and 51 RNA genes.

No MeSH data available.


Related in: MedlinePlus