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Genome sequence and description of Aeromicrobium massiliense sp. nov.

Ramasamy D, Kokcha S, Lagier JC, Nguyen TT, Raoult D, Fournier PE - Stand Genomic Sci (2012)

Bottom Line: Aeromicrobium massiliense is an aerobic rod-shaped gram-positive bacterium.Here we describe the features of this organism, together with the complete genome sequence and annotation.The 3,322,119 bp long genome contains 3,296 protein-coding and 51 RNA genes.

View Article: PubMed Central - PubMed

Affiliation: Aix-Marseille Université, Faculté de médecine, Marseille, France.

ABSTRACT
Aeromicrobium massiliense strain JC14(T)sp. nov. is the type strain of Aeromicrobium massiliense sp. nov., a new species within the genus Aeromicrobium. This strain, whose genome is described here, was isolated from the fecal microbiota of an asymptomatic patient. Aeromicrobium massiliense is an aerobic rod-shaped gram-positive bacterium. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 3,322,119 bp long genome contains 3,296 protein-coding and 51 RNA genes.

No MeSH data available.


Related in: MedlinePlus

Transmission electron microscopy of A. massiliense strain JC14T using a Morgani 268D (Philips) at an operating voltage of 60kV.The scale bar represents 500 nm.
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f3: Transmission electron microscopy of A. massiliense strain JC14T using a Morgani 268D (Philips) at an operating voltage of 60kV.The scale bar represents 500 nm.

Mentions: Different growth temperatures (25, 30, 37, 45, 50°C) were tested; no growth occurred at 50°C, very weak growth occurred at 45°C, and optimal growth was observed between 25 to 37°C. Colonies were light yellow and opaque with a diameter of 1 mm on 5% blood-enriched Columbia agar (BioMérieux). Growth of the strain was tested under anaerobic and microaerophilic conditions using GENbag anaer and GENbag microaer systems, respectively (BioMérieux), and in the presence of air, with or without 5% CO2. Optimal growth was achieved under aerobic conditions, with or without CO2, and weak growth occurred in microaerophilic conditions. No growth was observed under anaerobic conditions. Gram staining showed rod-shaped Gram-positive bacteria. A motility test was positive. Cells grown on agar are Gram-positive (Figure 2) and have a mean diameter of 1.04 µm and a mean length of 1.67 µm (Figure 3).


Genome sequence and description of Aeromicrobium massiliense sp. nov.

Ramasamy D, Kokcha S, Lagier JC, Nguyen TT, Raoult D, Fournier PE - Stand Genomic Sci (2012)

Transmission electron microscopy of A. massiliense strain JC14T using a Morgani 268D (Philips) at an operating voltage of 60kV.The scale bar represents 500 nm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3569385&req=5

f3: Transmission electron microscopy of A. massiliense strain JC14T using a Morgani 268D (Philips) at an operating voltage of 60kV.The scale bar represents 500 nm.
Mentions: Different growth temperatures (25, 30, 37, 45, 50°C) were tested; no growth occurred at 50°C, very weak growth occurred at 45°C, and optimal growth was observed between 25 to 37°C. Colonies were light yellow and opaque with a diameter of 1 mm on 5% blood-enriched Columbia agar (BioMérieux). Growth of the strain was tested under anaerobic and microaerophilic conditions using GENbag anaer and GENbag microaer systems, respectively (BioMérieux), and in the presence of air, with or without 5% CO2. Optimal growth was achieved under aerobic conditions, with or without CO2, and weak growth occurred in microaerophilic conditions. No growth was observed under anaerobic conditions. Gram staining showed rod-shaped Gram-positive bacteria. A motility test was positive. Cells grown on agar are Gram-positive (Figure 2) and have a mean diameter of 1.04 µm and a mean length of 1.67 µm (Figure 3).

Bottom Line: Aeromicrobium massiliense is an aerobic rod-shaped gram-positive bacterium.Here we describe the features of this organism, together with the complete genome sequence and annotation.The 3,322,119 bp long genome contains 3,296 protein-coding and 51 RNA genes.

View Article: PubMed Central - PubMed

Affiliation: Aix-Marseille Université, Faculté de médecine, Marseille, France.

ABSTRACT
Aeromicrobium massiliense strain JC14(T)sp. nov. is the type strain of Aeromicrobium massiliense sp. nov., a new species within the genus Aeromicrobium. This strain, whose genome is described here, was isolated from the fecal microbiota of an asymptomatic patient. Aeromicrobium massiliense is an aerobic rod-shaped gram-positive bacterium. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 3,322,119 bp long genome contains 3,296 protein-coding and 51 RNA genes.

No MeSH data available.


Related in: MedlinePlus