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Non contiguous-finished genome sequence and description of Peptoniphilus grossensis sp. nov.

Mishra AK, Hugon P, Robert C, Raoult D, Fournier PE - Stand Genomic Sci (2012)

Bottom Line: This strain, whose genome is described here, was isolated from the fecal flora of a 26-year-old woman suffering from morbid obesity.Here we describe the features of this organism, together with the complete genome sequence and annotation.The 2,101,866-bp long genome (1 chromosome but no plasmid) exhibits a G+C content of 33.9% and contains 2,041 protein-coding and 29 RNA genes, including 3 rRNA genes.

View Article: PubMed Central - PubMed

Affiliation: Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, UMR CNRS 7278.

ABSTRACT
Peptoniphilus grossensis strain ph5(T) sp. nov., is the type strain of Peptoniphilus grossensis sp. nov., a new species within the Peptoniphilus genus. This strain, whose genome is described here, was isolated from the fecal flora of a 26-year-old woman suffering from morbid obesity. P. grossensis strain ph5 is a Gram-positive obligate anaerobic coccus. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 2,101,866-bp long genome (1 chromosome but no plasmid) exhibits a G+C content of 33.9% and contains 2,041 protein-coding and 29 RNA genes, including 3 rRNA genes.

No MeSH data available.


Related in: MedlinePlus

Transmission electron microscopy of P. grossensis strain ph5T, using a Morgani 268D (Philips) at an operating voltage of 60kV. The scale bar represents 900 nm.
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f3: Transmission electron microscopy of P. grossensis strain ph5T, using a Morgani 268D (Philips) at an operating voltage of 60kV. The scale bar represents 900 nm.

Mentions: Different growth temperatures (25, 30, 37, 45°C) were tested; no growth occurred at 25°C, 30°C or 45°C. Growth only occurred at 37°C. Colonies were 2 mm in diameter on blood-enriched Columbia agar and Brain Heart Infusion (BHI) agar. Growth of the strain was tested under anaerobic and microaerophilic conditions using GENbag anaer and GENbag microaer systems, respectively (BioMérieux), and in the presence of air, with or without 5% CO2. Growth was achieved only anaerobically. Gram staining showed Gram-positive cocci able to form spores (Figure 2). The motility test was negative. Cells grown on agar had a mean diameter of 1.2 µm by electron microscopy and were mostly grouped in pairs, short chains or small clumps (Figure 3).


Non contiguous-finished genome sequence and description of Peptoniphilus grossensis sp. nov.

Mishra AK, Hugon P, Robert C, Raoult D, Fournier PE - Stand Genomic Sci (2012)

Transmission electron microscopy of P. grossensis strain ph5T, using a Morgani 268D (Philips) at an operating voltage of 60kV. The scale bar represents 900 nm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3569384&req=5

f3: Transmission electron microscopy of P. grossensis strain ph5T, using a Morgani 268D (Philips) at an operating voltage of 60kV. The scale bar represents 900 nm.
Mentions: Different growth temperatures (25, 30, 37, 45°C) were tested; no growth occurred at 25°C, 30°C or 45°C. Growth only occurred at 37°C. Colonies were 2 mm in diameter on blood-enriched Columbia agar and Brain Heart Infusion (BHI) agar. Growth of the strain was tested under anaerobic and microaerophilic conditions using GENbag anaer and GENbag microaer systems, respectively (BioMérieux), and in the presence of air, with or without 5% CO2. Growth was achieved only anaerobically. Gram staining showed Gram-positive cocci able to form spores (Figure 2). The motility test was negative. Cells grown on agar had a mean diameter of 1.2 µm by electron microscopy and were mostly grouped in pairs, short chains or small clumps (Figure 3).

Bottom Line: This strain, whose genome is described here, was isolated from the fecal flora of a 26-year-old woman suffering from morbid obesity.Here we describe the features of this organism, together with the complete genome sequence and annotation.The 2,101,866-bp long genome (1 chromosome but no plasmid) exhibits a G+C content of 33.9% and contains 2,041 protein-coding and 29 RNA genes, including 3 rRNA genes.

View Article: PubMed Central - PubMed

Affiliation: Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, UMR CNRS 7278.

ABSTRACT
Peptoniphilus grossensis strain ph5(T) sp. nov., is the type strain of Peptoniphilus grossensis sp. nov., a new species within the Peptoniphilus genus. This strain, whose genome is described here, was isolated from the fecal flora of a 26-year-old woman suffering from morbid obesity. P. grossensis strain ph5 is a Gram-positive obligate anaerobic coccus. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 2,101,866-bp long genome (1 chromosome but no plasmid) exhibits a G+C content of 33.9% and contains 2,041 protein-coding and 29 RNA genes, including 3 rRNA genes.

No MeSH data available.


Related in: MedlinePlus