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Complete genome sequence of the sulfate-reducing firmicute Desulfotomaculum ruminis type strain (DL(T)).

Spring S, Visser M, Lu M, Copeland A, Lapidus A, Lucas S, Cheng JF, Han C, Tapia R, Goodwin LA, Pitluck S, Ivanova N, Land M, Hauser L, Larimer F, Rohde M, Göker M, Detter JC, Kyrpides NC, Woyke T, Schaap PJ, Plugge CM, Muyzer G, Kuever J, Pereira IA, Parshina SN, Bernier-Latmani R, Stams AJ, Klenk HP - Stand Genomic Sci (2012)

Bottom Line: Desulfotomaculum ruminis Campbell and Postgate 1965 is a member of the large genus Desulfotomaculum which contains 30 species and is contained in the family Peptococcaceae.Here we describe the features of D. ruminis together with the complete genome sequence and annotation.The 3,969,014 bp long chromosome with a total of 3,901 protein-coding and 85 RNA genes is the second completed genome sequence of a type strain of the genus Desulfotomaculum to be published, and was sequenced as part of the DOE Joint Genome Institute Community Sequencing Program 2009.

View Article: PubMed Central - PubMed

Affiliation: Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.

ABSTRACT
Desulfotomaculum ruminis Campbell and Postgate 1965 is a member of the large genus Desulfotomaculum which contains 30 species and is contained in the family Peptococcaceae. This species is of interest because it represents one of the few sulfate-reducing bacteria that have been isolated from the rumen. Here we describe the features of D. ruminis together with the complete genome sequence and annotation. The 3,969,014 bp long chromosome with a total of 3,901 protein-coding and 85 RNA genes is the second completed genome sequence of a type strain of the genus Desulfotomaculum to be published, and was sequenced as part of the DOE Joint Genome Institute Community Sequencing Program 2009.

No MeSH data available.


Related in: MedlinePlus

Scanning electron micrograph of D. ruminis DLT.
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f2: Scanning electron micrograph of D. ruminis DLT.

Mentions: Cells of D. ruminis DLT are slightly curved rods with rounded ends 2-6 µm in length and 0.5-0.7 µm in width (Table 1 and Figure 2) [1,4]. Cells stain Gram-negative and form oval subterminal spores that slightly swell the cells. A slight tumbling motility is due to peritrichous flagellation [1]. Strain DLT grows optimally at 37°C, but not above 48°C [1]. The pH range for growth is 6.0-8.5 with an optimum between pH 6.0 and 7.0 [4]. D. ruminis strains are obligately anaerobic and can grow chemoheterotrophically with lactate, pyruvate, ethanol or alanine as well as mixotrophically with hydrogen or formate as electron donor and acetate as carbon source. In contrast to the distantly related D. acetoxidans, strains of D. ruminis oxidize substrates incompletely to acetate and cannot grow autotrophically [4]. Suitable electron acceptors are sulfate, thiosulfate and sulfite, but not elemental sulfur or nitrate [1,26]. Fermentative growth with pyruvate as sole substrate is also possible [26].


Complete genome sequence of the sulfate-reducing firmicute Desulfotomaculum ruminis type strain (DL(T)).

Spring S, Visser M, Lu M, Copeland A, Lapidus A, Lucas S, Cheng JF, Han C, Tapia R, Goodwin LA, Pitluck S, Ivanova N, Land M, Hauser L, Larimer F, Rohde M, Göker M, Detter JC, Kyrpides NC, Woyke T, Schaap PJ, Plugge CM, Muyzer G, Kuever J, Pereira IA, Parshina SN, Bernier-Latmani R, Stams AJ, Klenk HP - Stand Genomic Sci (2012)

Scanning electron micrograph of D. ruminis DLT.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3569383&req=5

f2: Scanning electron micrograph of D. ruminis DLT.
Mentions: Cells of D. ruminis DLT are slightly curved rods with rounded ends 2-6 µm in length and 0.5-0.7 µm in width (Table 1 and Figure 2) [1,4]. Cells stain Gram-negative and form oval subterminal spores that slightly swell the cells. A slight tumbling motility is due to peritrichous flagellation [1]. Strain DLT grows optimally at 37°C, but not above 48°C [1]. The pH range for growth is 6.0-8.5 with an optimum between pH 6.0 and 7.0 [4]. D. ruminis strains are obligately anaerobic and can grow chemoheterotrophically with lactate, pyruvate, ethanol or alanine as well as mixotrophically with hydrogen or formate as electron donor and acetate as carbon source. In contrast to the distantly related D. acetoxidans, strains of D. ruminis oxidize substrates incompletely to acetate and cannot grow autotrophically [4]. Suitable electron acceptors are sulfate, thiosulfate and sulfite, but not elemental sulfur or nitrate [1,26]. Fermentative growth with pyruvate as sole substrate is also possible [26].

Bottom Line: Desulfotomaculum ruminis Campbell and Postgate 1965 is a member of the large genus Desulfotomaculum which contains 30 species and is contained in the family Peptococcaceae.Here we describe the features of D. ruminis together with the complete genome sequence and annotation.The 3,969,014 bp long chromosome with a total of 3,901 protein-coding and 85 RNA genes is the second completed genome sequence of a type strain of the genus Desulfotomaculum to be published, and was sequenced as part of the DOE Joint Genome Institute Community Sequencing Program 2009.

View Article: PubMed Central - PubMed

Affiliation: Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.

ABSTRACT
Desulfotomaculum ruminis Campbell and Postgate 1965 is a member of the large genus Desulfotomaculum which contains 30 species and is contained in the family Peptococcaceae. This species is of interest because it represents one of the few sulfate-reducing bacteria that have been isolated from the rumen. Here we describe the features of D. ruminis together with the complete genome sequence and annotation. The 3,969,014 bp long chromosome with a total of 3,901 protein-coding and 85 RNA genes is the second completed genome sequence of a type strain of the genus Desulfotomaculum to be published, and was sequenced as part of the DOE Joint Genome Institute Community Sequencing Program 2009.

No MeSH data available.


Related in: MedlinePlus