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Effects of sinapic Acid of 4 vessel occlusion model-induced ischemia and cognitive impairments in the rat.

Kim YO, Lee SW, Oh MS, Lee HJ - Clin Psychopharmacol Neurosci (2011)

Bottom Line: To examine the neuroprotective effects of SA, SA was administrated for 14 d before 4-vessel occlusion.In this study, the efficacy of SA for the prevention of neuronal damage and for the reduction of memory impairment was investigated.The results indicate that SA confers significant neuroprotection especially for ischemic hippocampal neurons.

View Article: PubMed Central - PubMed

Affiliation: Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration, Eumseong, Korea.

ABSTRACT

Objective: Sinapic acid (SA, Sinapine), small naturally occurring hydroxycinnamic acid, has a GABA(A) receptor agonistic property and free radical scavenging activity. We examined potential neuroprotective effects of sinapic acid (SA) using global cerebral ischemia animal model.

Methods: MTT assay was performed to determine cytotoxic effects of SA. To examine the neuroprotective effects of SA, SA was administrated for 14 d before 4-vessel occlusion. Also, to determine whether SA prevents cognitive impairment, Morris water maze was performed.

Results: In this study, the efficacy of SA for the prevention of neuronal damage and for the reduction of memory impairment was investigated.

Conclusion: The results indicate that SA confers significant neuroprotection especially for ischemic hippocampal neurons.

No MeSH data available.


Related in: MedlinePlus

Cytotoxicity by SA was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. SH-SY5Y cells. These results show that SA can be used safely.
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Figure 1: Cytotoxicity by SA was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. SH-SY5Y cells. These results show that SA can be used safely.

Mentions: To determine cytotoxic effects of SA on SH-SY5Y cells, cells were incubated with various concentrations of SA (0, 1, 10, and 100µg/ml) for 24 h. In 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays, the viabilities of SH-SY5Y cells exposed to SA for 24 h were 100%, 101.1%, 103.2%, and 105.2%, respectively (Fig. 1). The treatment of SA did not induce cytotoxic effects on SH-SY5Y cells. These results show that SA can be used safely.


Effects of sinapic Acid of 4 vessel occlusion model-induced ischemia and cognitive impairments in the rat.

Kim YO, Lee SW, Oh MS, Lee HJ - Clin Psychopharmacol Neurosci (2011)

Cytotoxicity by SA was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. SH-SY5Y cells. These results show that SA can be used safely.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3569082&req=5

Figure 1: Cytotoxicity by SA was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. SH-SY5Y cells. These results show that SA can be used safely.
Mentions: To determine cytotoxic effects of SA on SH-SY5Y cells, cells were incubated with various concentrations of SA (0, 1, 10, and 100µg/ml) for 24 h. In 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays, the viabilities of SH-SY5Y cells exposed to SA for 24 h were 100%, 101.1%, 103.2%, and 105.2%, respectively (Fig. 1). The treatment of SA did not induce cytotoxic effects on SH-SY5Y cells. These results show that SA can be used safely.

Bottom Line: To examine the neuroprotective effects of SA, SA was administrated for 14 d before 4-vessel occlusion.In this study, the efficacy of SA for the prevention of neuronal damage and for the reduction of memory impairment was investigated.The results indicate that SA confers significant neuroprotection especially for ischemic hippocampal neurons.

View Article: PubMed Central - PubMed

Affiliation: Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration, Eumseong, Korea.

ABSTRACT

Objective: Sinapic acid (SA, Sinapine), small naturally occurring hydroxycinnamic acid, has a GABA(A) receptor agonistic property and free radical scavenging activity. We examined potential neuroprotective effects of sinapic acid (SA) using global cerebral ischemia animal model.

Methods: MTT assay was performed to determine cytotoxic effects of SA. To examine the neuroprotective effects of SA, SA was administrated for 14 d before 4-vessel occlusion. Also, to determine whether SA prevents cognitive impairment, Morris water maze was performed.

Results: In this study, the efficacy of SA for the prevention of neuronal damage and for the reduction of memory impairment was investigated.

Conclusion: The results indicate that SA confers significant neuroprotection especially for ischemic hippocampal neurons.

No MeSH data available.


Related in: MedlinePlus