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Rapid changes in d1 and d2 dopamine receptor binding in striatal subregions after a single dose of phencyclidine.

Dalton VS, Zavitsanou K - Clin Psychopharmacol Neurosci (2011)

Bottom Line: In vitro autoradiography was carried out using [(3)H] SCH 23390 and [(3)H] raclopride that target D1R and D2R respectively, in cryostat brain sections.Two way analysis of variance (ANOVA), revealed an overall effect of PCP treatment (F [1,63]=9.065; p=0.004) on D1R binding with an 18% decrease (p<0.01) in binding in the medial caudate putamen.PCP treatment also had an overall effect on D2R binding (F [1,47]=5.450; p=0.024) and a trend for an increase in D2R binding across all the brain regions examined.

View Article: PubMed Central - PubMed

Affiliation: Schizophrenia Research Institute, Australian Nuclear Science and Technology Organisation, Sydney, Australia. ; ANSTO LifeSciences, Australian Nuclear Science and Technology Organisation, Sydney, Australia.

ABSTRACT

Objective: In humans, a single exposure to phencyclidine (PCP) can induce a schizophrenia-like psychosis which can persist for up to two weeks. In rats, an acute dose of PCP increases dopaminergic activity and causes changes in dopamine related behaviours some of which are sexually dimorphic. To better understand the effects of PCP on dopamine receptor adaptations in the short term we examined dopamine D1-like receptors (D1R) and D2-like receptors (D2R) in the mesolimbic and nigrostriatal dopamine pathways, 4 hours after exposure to PCP in female rats.

Methods: Animals received a single dose of 40 mg/kg PCP and were sacrificed 4 hours later. In vitro autoradiography was carried out using [(3)H] SCH 23390 and [(3)H] raclopride that target D1R and D2R respectively, in cryostat brain sections.

Results: Two way analysis of variance (ANOVA), revealed an overall effect of PCP treatment (F [1,63]=9.065; p=0.004) on D1R binding with an 18% decrease (p<0.01) in binding in the medial caudate putamen. PCP treatment also had an overall effect on D2R binding (F [1,47]=5.450; p=0.024) and a trend for an increase in D2R binding across all the brain regions examined.

Conclusion: These results suggest opposing D1R and D2R adaptations in striatal subregions of female rats following acute exposure to PCP that may occur through indirect mechanisms.

No MeSH data available.


Related in: MedlinePlus

[3H] raclopride binding in fmoles/mg tissue equivalent (TE) in female rats treated with vehicle or PCP. *Statistically significant effect of treatment revealed by two way ANOVA (F (1, 47)=5.450, p=0.024). See Fig. 1 for abbreviations.
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Figure 3: [3H] raclopride binding in fmoles/mg tissue equivalent (TE) in female rats treated with vehicle or PCP. *Statistically significant effect of treatment revealed by two way ANOVA (F (1, 47)=5.450, p=0.024). See Fig. 1 for abbreviations.

Mentions: Statistical analysis revealed significant variation in [3H] raclopride binding with treatment (F [1, 47]=5.450, p=0.024) and brain region (F [3, 47]=64.307, p<0.0001). A significant interaction between treatment and region was not found. Post hoc tests did not detect any statistically significant individual changes in binding in brain region or treatment group (Fig. 1, 3) although the increase in the lateral caudate putamen approached significance (p=0.086). These results indicate that non-significant increases in [3H] raclopride binding (medial caudate putamen=0.75%; lateral caudate putamen=10%; nucleus accumbens=13%; olfactory tubercle=15%) occurred in the regions assessed in PCP treated rats compared to controls, the sum of these changes resulting in the significant, region-wide effect of PCP treatment on [3H] raclopride binding.29)


Rapid changes in d1 and d2 dopamine receptor binding in striatal subregions after a single dose of phencyclidine.

Dalton VS, Zavitsanou K - Clin Psychopharmacol Neurosci (2011)

[3H] raclopride binding in fmoles/mg tissue equivalent (TE) in female rats treated with vehicle or PCP. *Statistically significant effect of treatment revealed by two way ANOVA (F (1, 47)=5.450, p=0.024). See Fig. 1 for abbreviations.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3569081&req=5

Figure 3: [3H] raclopride binding in fmoles/mg tissue equivalent (TE) in female rats treated with vehicle or PCP. *Statistically significant effect of treatment revealed by two way ANOVA (F (1, 47)=5.450, p=0.024). See Fig. 1 for abbreviations.
Mentions: Statistical analysis revealed significant variation in [3H] raclopride binding with treatment (F [1, 47]=5.450, p=0.024) and brain region (F [3, 47]=64.307, p<0.0001). A significant interaction between treatment and region was not found. Post hoc tests did not detect any statistically significant individual changes in binding in brain region or treatment group (Fig. 1, 3) although the increase in the lateral caudate putamen approached significance (p=0.086). These results indicate that non-significant increases in [3H] raclopride binding (medial caudate putamen=0.75%; lateral caudate putamen=10%; nucleus accumbens=13%; olfactory tubercle=15%) occurred in the regions assessed in PCP treated rats compared to controls, the sum of these changes resulting in the significant, region-wide effect of PCP treatment on [3H] raclopride binding.29)

Bottom Line: In vitro autoradiography was carried out using [(3)H] SCH 23390 and [(3)H] raclopride that target D1R and D2R respectively, in cryostat brain sections.Two way analysis of variance (ANOVA), revealed an overall effect of PCP treatment (F [1,63]=9.065; p=0.004) on D1R binding with an 18% decrease (p<0.01) in binding in the medial caudate putamen.PCP treatment also had an overall effect on D2R binding (F [1,47]=5.450; p=0.024) and a trend for an increase in D2R binding across all the brain regions examined.

View Article: PubMed Central - PubMed

Affiliation: Schizophrenia Research Institute, Australian Nuclear Science and Technology Organisation, Sydney, Australia. ; ANSTO LifeSciences, Australian Nuclear Science and Technology Organisation, Sydney, Australia.

ABSTRACT

Objective: In humans, a single exposure to phencyclidine (PCP) can induce a schizophrenia-like psychosis which can persist for up to two weeks. In rats, an acute dose of PCP increases dopaminergic activity and causes changes in dopamine related behaviours some of which are sexually dimorphic. To better understand the effects of PCP on dopamine receptor adaptations in the short term we examined dopamine D1-like receptors (D1R) and D2-like receptors (D2R) in the mesolimbic and nigrostriatal dopamine pathways, 4 hours after exposure to PCP in female rats.

Methods: Animals received a single dose of 40 mg/kg PCP and were sacrificed 4 hours later. In vitro autoradiography was carried out using [(3)H] SCH 23390 and [(3)H] raclopride that target D1R and D2R respectively, in cryostat brain sections.

Results: Two way analysis of variance (ANOVA), revealed an overall effect of PCP treatment (F [1,63]=9.065; p=0.004) on D1R binding with an 18% decrease (p<0.01) in binding in the medial caudate putamen. PCP treatment also had an overall effect on D2R binding (F [1,47]=5.450; p=0.024) and a trend for an increase in D2R binding across all the brain regions examined.

Conclusion: These results suggest opposing D1R and D2R adaptations in striatal subregions of female rats following acute exposure to PCP that may occur through indirect mechanisms.

No MeSH data available.


Related in: MedlinePlus