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Spinal nerve injury causes upregulation of ErbB2 and ErbB3 receptors in rat dorsal root ganglia.

Mizobuchi S, Kanzaki H, Omiya H, Matsuoka Y, Obata N, Kaku R, Nakajima H, Ouchida M, Morita K - J Pain Res (2013)

Bottom Line: It is generally known that peripheral nerve injury causes changes in expression of some growth factors in the dorsal root ganglion.The expression of ErbB2 and ErbB3 was observed in the dorsal root ganglia of the mature rat, despite ErbB1 and ErbB4 showing only subtle expression.We also demonstrated that peripheral nerve injury induced significant increases in ErbB2 and ErbB3 in the ipsilateral dorsal root ganglion as compared with uninjured nerve.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology and Resuscitology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.

ABSTRACT
It is generally known that peripheral nerve injury causes changes in expression of some growth factors in the dorsal root ganglion. Altered expression of ErbB receptors, a well-known growth factor in somatic cells, reportedly follows peripheral nerve injury in the spinal dorsal horn; however, it remains unknown whether the expression of these receptors is altered in the dorsal root ganglion after nerve injury. Therefore, this study examined the gene expression profiles of ErbB receptors in bilateral lumbar (L)4/L5 dorsal root ganglia, using L5-selective spinal nerve ligation in model rats as a peripheral nerve injury model. The expression of ErbB2 and ErbB3 was observed in the dorsal root ganglia of the mature rat, despite ErbB1 and ErbB4 showing only subtle expression. We also demonstrated that peripheral nerve injury induced significant increases in ErbB2 and ErbB3 in the ipsilateral dorsal root ganglion as compared with uninjured nerve. Expression changes in ErbB receptors appear to play important roles in nerve injury and subsequent nerve regeneration.

No MeSH data available.


Related in: MedlinePlus

Time course and the expression of ErbBs in bilateral L4/L5 DRG of SNL model rats. Expression of the (A) EGFR (B) ErbB2 (C) ErbB3 (D) ErbB4 mRNA; expressions were tested before surgery and at 7 and 14 days after surgery.Notes: All of the expression levels were normalized to that of the GADPH. Data are mean ± SD (n = 7). *P < 0.05 vs contralateral side.Abbreviations: ErbB, erythroblastic leukemia viral oncogene homolog; L, lumbar; DRG, dorsal root ganglion; SNL, spinal nerve ligation; GAPDH, glyceraldehyde 3-phosphate dehydrogenase gene; SD, standard deviation; EGFR, epidermal growth factor receptor; Ipsi, ipsilateral side; Contra, contralateral side.
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f5-jpr-6-087: Time course and the expression of ErbBs in bilateral L4/L5 DRG of SNL model rats. Expression of the (A) EGFR (B) ErbB2 (C) ErbB3 (D) ErbB4 mRNA; expressions were tested before surgery and at 7 and 14 days after surgery.Notes: All of the expression levels were normalized to that of the GADPH. Data are mean ± SD (n = 7). *P < 0.05 vs contralateral side.Abbreviations: ErbB, erythroblastic leukemia viral oncogene homolog; L, lumbar; DRG, dorsal root ganglion; SNL, spinal nerve ligation; GAPDH, glyceraldehyde 3-phosphate dehydrogenase gene; SD, standard deviation; EGFR, epidermal growth factor receptor; Ipsi, ipsilateral side; Contra, contralateral side.

Mentions: We also investigated the time-dependent expression changes in expression ErbB receptors. Significantly increased expression of ErbB2 and ErbB3 was observed in the ipsilateral L5 DRGs at day 14, whereas the expression levels did not change until day 7 (Figure 5). Sham surgery did not induce any changes in ErbB receptor expression in either L4/L5 DRGs at the mRNA level. Furthermore, basal expression of each gene was not altered. The time course of ErbB2 and ErbB3 upregulation was not consistent with behavioral development (Figure 1A). These data suggest that upregulation of ErbB2 and ErbB3 in the L5 DRG is correlated with the delayed event to response to spinal nerve injury.


Spinal nerve injury causes upregulation of ErbB2 and ErbB3 receptors in rat dorsal root ganglia.

Mizobuchi S, Kanzaki H, Omiya H, Matsuoka Y, Obata N, Kaku R, Nakajima H, Ouchida M, Morita K - J Pain Res (2013)

Time course and the expression of ErbBs in bilateral L4/L5 DRG of SNL model rats. Expression of the (A) EGFR (B) ErbB2 (C) ErbB3 (D) ErbB4 mRNA; expressions were tested before surgery and at 7 and 14 days after surgery.Notes: All of the expression levels were normalized to that of the GADPH. Data are mean ± SD (n = 7). *P < 0.05 vs contralateral side.Abbreviations: ErbB, erythroblastic leukemia viral oncogene homolog; L, lumbar; DRG, dorsal root ganglion; SNL, spinal nerve ligation; GAPDH, glyceraldehyde 3-phosphate dehydrogenase gene; SD, standard deviation; EGFR, epidermal growth factor receptor; Ipsi, ipsilateral side; Contra, contralateral side.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3569052&req=5

f5-jpr-6-087: Time course and the expression of ErbBs in bilateral L4/L5 DRG of SNL model rats. Expression of the (A) EGFR (B) ErbB2 (C) ErbB3 (D) ErbB4 mRNA; expressions were tested before surgery and at 7 and 14 days after surgery.Notes: All of the expression levels were normalized to that of the GADPH. Data are mean ± SD (n = 7). *P < 0.05 vs contralateral side.Abbreviations: ErbB, erythroblastic leukemia viral oncogene homolog; L, lumbar; DRG, dorsal root ganglion; SNL, spinal nerve ligation; GAPDH, glyceraldehyde 3-phosphate dehydrogenase gene; SD, standard deviation; EGFR, epidermal growth factor receptor; Ipsi, ipsilateral side; Contra, contralateral side.
Mentions: We also investigated the time-dependent expression changes in expression ErbB receptors. Significantly increased expression of ErbB2 and ErbB3 was observed in the ipsilateral L5 DRGs at day 14, whereas the expression levels did not change until day 7 (Figure 5). Sham surgery did not induce any changes in ErbB receptor expression in either L4/L5 DRGs at the mRNA level. Furthermore, basal expression of each gene was not altered. The time course of ErbB2 and ErbB3 upregulation was not consistent with behavioral development (Figure 1A). These data suggest that upregulation of ErbB2 and ErbB3 in the L5 DRG is correlated with the delayed event to response to spinal nerve injury.

Bottom Line: It is generally known that peripheral nerve injury causes changes in expression of some growth factors in the dorsal root ganglion.The expression of ErbB2 and ErbB3 was observed in the dorsal root ganglia of the mature rat, despite ErbB1 and ErbB4 showing only subtle expression.We also demonstrated that peripheral nerve injury induced significant increases in ErbB2 and ErbB3 in the ipsilateral dorsal root ganglion as compared with uninjured nerve.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology and Resuscitology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.

ABSTRACT
It is generally known that peripheral nerve injury causes changes in expression of some growth factors in the dorsal root ganglion. Altered expression of ErbB receptors, a well-known growth factor in somatic cells, reportedly follows peripheral nerve injury in the spinal dorsal horn; however, it remains unknown whether the expression of these receptors is altered in the dorsal root ganglion after nerve injury. Therefore, this study examined the gene expression profiles of ErbB receptors in bilateral lumbar (L)4/L5 dorsal root ganglia, using L5-selective spinal nerve ligation in model rats as a peripheral nerve injury model. The expression of ErbB2 and ErbB3 was observed in the dorsal root ganglia of the mature rat, despite ErbB1 and ErbB4 showing only subtle expression. We also demonstrated that peripheral nerve injury induced significant increases in ErbB2 and ErbB3 in the ipsilateral dorsal root ganglion as compared with uninjured nerve. Expression changes in ErbB receptors appear to play important roles in nerve injury and subsequent nerve regeneration.

No MeSH data available.


Related in: MedlinePlus