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Spinal nerve injury causes upregulation of ErbB2 and ErbB3 receptors in rat dorsal root ganglia.

Mizobuchi S, Kanzaki H, Omiya H, Matsuoka Y, Obata N, Kaku R, Nakajima H, Ouchida M, Morita K - J Pain Res (2013)

Bottom Line: It is generally known that peripheral nerve injury causes changes in expression of some growth factors in the dorsal root ganglion.The expression of ErbB2 and ErbB3 was observed in the dorsal root ganglia of the mature rat, despite ErbB1 and ErbB4 showing only subtle expression.We also demonstrated that peripheral nerve injury induced significant increases in ErbB2 and ErbB3 in the ipsilateral dorsal root ganglion as compared with uninjured nerve.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology and Resuscitology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.

ABSTRACT
It is generally known that peripheral nerve injury causes changes in expression of some growth factors in the dorsal root ganglion. Altered expression of ErbB receptors, a well-known growth factor in somatic cells, reportedly follows peripheral nerve injury in the spinal dorsal horn; however, it remains unknown whether the expression of these receptors is altered in the dorsal root ganglion after nerve injury. Therefore, this study examined the gene expression profiles of ErbB receptors in bilateral lumbar (L)4/L5 dorsal root ganglia, using L5-selective spinal nerve ligation in model rats as a peripheral nerve injury model. The expression of ErbB2 and ErbB3 was observed in the dorsal root ganglia of the mature rat, despite ErbB1 and ErbB4 showing only subtle expression. We also demonstrated that peripheral nerve injury induced significant increases in ErbB2 and ErbB3 in the ipsilateral dorsal root ganglion as compared with uninjured nerve. Expression changes in ErbB receptors appear to play important roles in nerve injury and subsequent nerve regeneration.

No MeSH data available.


Related in: MedlinePlus

Ratio of expression of the EGFR, ErbB2, ErbB3, and ErbB4 transcripts in the bilateral L4 and L5 DRG of CFA model rats, sham-CFA rats and naïve rats. Expression of the (A) EGFR; (B) ErbB2; (C) ErbB3; and (D) ErbB4 mRNA; all of the expression levels were normalized to that of GADPH.Notes: Data are mean ± SD. CFA group and sham-CFA group: n = 6; naïve group: n = 13.Abbreviations: EGFR, epidermal growth factor receptor; ErbB, erythroblastic leukemia viral oncogene homolog; L, lumbar; DRG, dorsal root ganglion; CFA, complete Freund’s adjuvant; GAPDH, glyceraldehyde 3-phosphate dehydrogenase gene; SD, standard deviation; I, ipsilateral side; C, contralateral side.
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f3-jpr-6-087: Ratio of expression of the EGFR, ErbB2, ErbB3, and ErbB4 transcripts in the bilateral L4 and L5 DRG of CFA model rats, sham-CFA rats and naïve rats. Expression of the (A) EGFR; (B) ErbB2; (C) ErbB3; and (D) ErbB4 mRNA; all of the expression levels were normalized to that of GADPH.Notes: Data are mean ± SD. CFA group and sham-CFA group: n = 6; naïve group: n = 13.Abbreviations: EGFR, epidermal growth factor receptor; ErbB, erythroblastic leukemia viral oncogene homolog; L, lumbar; DRG, dorsal root ganglion; CFA, complete Freund’s adjuvant; GAPDH, glyceraldehyde 3-phosphate dehydrogenase gene; SD, standard deviation; I, ipsilateral side; C, contralateral side.

Mentions: The expression profiles of ErbB receptors were examined by quantitative real-time PCR in ipsilateral and contralateral L4/L5 DRGs of the SNL model, sham-SNL, and naïve rats (Figure 2). Expression of ErbB2 and ErbB3 was observed in both L4/L5 DRGs of mature naïve rats, despite the subtle expression of ErbB1 and ErbB4. The expression of ErbB2 and ErbB3 was significantly elevated in ipsilateral L5 DRGs after spinal nerve injury (199% of the contralateral value, P = 0.0019; and 196%, P = 0.0005, respectively) (Figure 2). In contrast, the expression of ErbB receptors was not altered in ipsilateral L4 DRGs after spinal nerve injury. No expression changes were observed in the negative controls, sham-SNL, CFA, or sham-CFA (Figures 2 and 3). ErbB3 overexpression was also confirmed at the protein level, by Western blot (229% of contralateral value, P = 0.0020), while ErbB2 expression could not be quantified due to the low protein abundance (Figure 4). This result is consistent with a finding that the expression of ErbB3 is higher than ErbB2 at mRNA (Figures 2 and 4).


Spinal nerve injury causes upregulation of ErbB2 and ErbB3 receptors in rat dorsal root ganglia.

Mizobuchi S, Kanzaki H, Omiya H, Matsuoka Y, Obata N, Kaku R, Nakajima H, Ouchida M, Morita K - J Pain Res (2013)

Ratio of expression of the EGFR, ErbB2, ErbB3, and ErbB4 transcripts in the bilateral L4 and L5 DRG of CFA model rats, sham-CFA rats and naïve rats. Expression of the (A) EGFR; (B) ErbB2; (C) ErbB3; and (D) ErbB4 mRNA; all of the expression levels were normalized to that of GADPH.Notes: Data are mean ± SD. CFA group and sham-CFA group: n = 6; naïve group: n = 13.Abbreviations: EGFR, epidermal growth factor receptor; ErbB, erythroblastic leukemia viral oncogene homolog; L, lumbar; DRG, dorsal root ganglion; CFA, complete Freund’s adjuvant; GAPDH, glyceraldehyde 3-phosphate dehydrogenase gene; SD, standard deviation; I, ipsilateral side; C, contralateral side.
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Related In: Results  -  Collection

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f3-jpr-6-087: Ratio of expression of the EGFR, ErbB2, ErbB3, and ErbB4 transcripts in the bilateral L4 and L5 DRG of CFA model rats, sham-CFA rats and naïve rats. Expression of the (A) EGFR; (B) ErbB2; (C) ErbB3; and (D) ErbB4 mRNA; all of the expression levels were normalized to that of GADPH.Notes: Data are mean ± SD. CFA group and sham-CFA group: n = 6; naïve group: n = 13.Abbreviations: EGFR, epidermal growth factor receptor; ErbB, erythroblastic leukemia viral oncogene homolog; L, lumbar; DRG, dorsal root ganglion; CFA, complete Freund’s adjuvant; GAPDH, glyceraldehyde 3-phosphate dehydrogenase gene; SD, standard deviation; I, ipsilateral side; C, contralateral side.
Mentions: The expression profiles of ErbB receptors were examined by quantitative real-time PCR in ipsilateral and contralateral L4/L5 DRGs of the SNL model, sham-SNL, and naïve rats (Figure 2). Expression of ErbB2 and ErbB3 was observed in both L4/L5 DRGs of mature naïve rats, despite the subtle expression of ErbB1 and ErbB4. The expression of ErbB2 and ErbB3 was significantly elevated in ipsilateral L5 DRGs after spinal nerve injury (199% of the contralateral value, P = 0.0019; and 196%, P = 0.0005, respectively) (Figure 2). In contrast, the expression of ErbB receptors was not altered in ipsilateral L4 DRGs after spinal nerve injury. No expression changes were observed in the negative controls, sham-SNL, CFA, or sham-CFA (Figures 2 and 3). ErbB3 overexpression was also confirmed at the protein level, by Western blot (229% of contralateral value, P = 0.0020), while ErbB2 expression could not be quantified due to the low protein abundance (Figure 4). This result is consistent with a finding that the expression of ErbB3 is higher than ErbB2 at mRNA (Figures 2 and 4).

Bottom Line: It is generally known that peripheral nerve injury causes changes in expression of some growth factors in the dorsal root ganglion.The expression of ErbB2 and ErbB3 was observed in the dorsal root ganglia of the mature rat, despite ErbB1 and ErbB4 showing only subtle expression.We also demonstrated that peripheral nerve injury induced significant increases in ErbB2 and ErbB3 in the ipsilateral dorsal root ganglion as compared with uninjured nerve.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology and Resuscitology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.

ABSTRACT
It is generally known that peripheral nerve injury causes changes in expression of some growth factors in the dorsal root ganglion. Altered expression of ErbB receptors, a well-known growth factor in somatic cells, reportedly follows peripheral nerve injury in the spinal dorsal horn; however, it remains unknown whether the expression of these receptors is altered in the dorsal root ganglion after nerve injury. Therefore, this study examined the gene expression profiles of ErbB receptors in bilateral lumbar (L)4/L5 dorsal root ganglia, using L5-selective spinal nerve ligation in model rats as a peripheral nerve injury model. The expression of ErbB2 and ErbB3 was observed in the dorsal root ganglia of the mature rat, despite ErbB1 and ErbB4 showing only subtle expression. We also demonstrated that peripheral nerve injury induced significant increases in ErbB2 and ErbB3 in the ipsilateral dorsal root ganglion as compared with uninjured nerve. Expression changes in ErbB receptors appear to play important roles in nerve injury and subsequent nerve regeneration.

No MeSH data available.


Related in: MedlinePlus