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Spinal nerve injury causes upregulation of ErbB2 and ErbB3 receptors in rat dorsal root ganglia.

Mizobuchi S, Kanzaki H, Omiya H, Matsuoka Y, Obata N, Kaku R, Nakajima H, Ouchida M, Morita K - J Pain Res (2013)

Bottom Line: It is generally known that peripheral nerve injury causes changes in expression of some growth factors in the dorsal root ganglion.The expression of ErbB2 and ErbB3 was observed in the dorsal root ganglia of the mature rat, despite ErbB1 and ErbB4 showing only subtle expression.We also demonstrated that peripheral nerve injury induced significant increases in ErbB2 and ErbB3 in the ipsilateral dorsal root ganglion as compared with uninjured nerve.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology and Resuscitology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.

ABSTRACT
It is generally known that peripheral nerve injury causes changes in expression of some growth factors in the dorsal root ganglion. Altered expression of ErbB receptors, a well-known growth factor in somatic cells, reportedly follows peripheral nerve injury in the spinal dorsal horn; however, it remains unknown whether the expression of these receptors is altered in the dorsal root ganglion after nerve injury. Therefore, this study examined the gene expression profiles of ErbB receptors in bilateral lumbar (L)4/L5 dorsal root ganglia, using L5-selective spinal nerve ligation in model rats as a peripheral nerve injury model. The expression of ErbB2 and ErbB3 was observed in the dorsal root ganglia of the mature rat, despite ErbB1 and ErbB4 showing only subtle expression. We also demonstrated that peripheral nerve injury induced significant increases in ErbB2 and ErbB3 in the ipsilateral dorsal root ganglion as compared with uninjured nerve. Expression changes in ErbB receptors appear to play important roles in nerve injury and subsequent nerve regeneration.

No MeSH data available.


Related in: MedlinePlus

(A) Behavioral assessment of the L5 spinal nerve ligation model (n = 7). There was no significant difference in 50% PWT between the ipsilateral and contralateral PWT of the sham group or between the contralateral sides of the SNL and sham groups; (B) behavioral assessment of the CFA model rats (n = 6). There was no significant difference in 50% PWT between the ipsilateral and contralateral PWT of the sham group or between the contralateral sides of the CFA and sham groups.Notes: The mechanical sensitivity of the hind paw was determined as the 50% PWT. Data are mean ± SD, *P < 0.05 (A) vs the contralateral PWT of the SNL model; (B) vs the contralateral PWT of the CFA model rat.Abbreviations: L, lumbar; SNL, spinal nerve ligation; PWT, paw withdrawal threshold; CFA, complete Freund’s adjuvant; SD, standard deviation; Ipsi, ipsilateral side; Contra, contralateral side.
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f1-jpr-6-087: (A) Behavioral assessment of the L5 spinal nerve ligation model (n = 7). There was no significant difference in 50% PWT between the ipsilateral and contralateral PWT of the sham group or between the contralateral sides of the SNL and sham groups; (B) behavioral assessment of the CFA model rats (n = 6). There was no significant difference in 50% PWT between the ipsilateral and contralateral PWT of the sham group or between the contralateral sides of the CFA and sham groups.Notes: The mechanical sensitivity of the hind paw was determined as the 50% PWT. Data are mean ± SD, *P < 0.05 (A) vs the contralateral PWT of the SNL model; (B) vs the contralateral PWT of the CFA model rat.Abbreviations: L, lumbar; SNL, spinal nerve ligation; PWT, paw withdrawal threshold; CFA, complete Freund’s adjuvant; SD, standard deviation; Ipsi, ipsilateral side; Contra, contralateral side.

Mentions: L5SNL model rats are well-established as a neuropathic pain model that shows hyperalgesia, without phenomena such as paralysis, when spinal nerve ligation/injury is successfully induced.12 To investigate hyperalgesia in L5SNL rats, we performed behavioral assessments with the von-Frey test before and after L5SNL. We used a CFA model, which shows hyperalgesia as a non-nerve injury.13 Mechanical allodynia was observed in L5SNL model rats throughout the examination period (days 1–14 after L5SNL). The CFA injection induced allodynia at 3 days after treatment. Prior to SNL and intraplantar injection of CFA, there were no differences in 50% PWT on the bilateral hind paws (ipsilateral 13.6 ± 1.8 g vs contralateral 13.6 ± 1.8 g; ipsilateral 15.0 ± 0 g vs contralateral 15.0 ± 0 g, respectively). The 50% PWTs in the SNL group were 3.1 ± 0.8, 2.8 ± 0.9, 2.5 ± 1.4, 3.2 ± 1.2, 2.1 ± 0.4, and 2.5 ± 1.0 g at 1, 3, 7, 9, 11, and 14 days after surgery, respectively (Figure 1A). Significantly decreased 50% PWTs were observed from days 1 through 14 after surgery. In the CFA group, the ipsilateral 50% PWT was also significantly lower on days 1 and 3 (2.8 ± 1.8 g and 4.2 ± 3.8 g, respectively) (Figure 1B). There were no changes in the 50% PWT on the contralateral side in any of all the groups. There were also no changes in 50% PWT on either the ipsilateral or contralateral sides in the sham and naïve groups. The model rats were thus validated based on these results.


Spinal nerve injury causes upregulation of ErbB2 and ErbB3 receptors in rat dorsal root ganglia.

Mizobuchi S, Kanzaki H, Omiya H, Matsuoka Y, Obata N, Kaku R, Nakajima H, Ouchida M, Morita K - J Pain Res (2013)

(A) Behavioral assessment of the L5 spinal nerve ligation model (n = 7). There was no significant difference in 50% PWT between the ipsilateral and contralateral PWT of the sham group or between the contralateral sides of the SNL and sham groups; (B) behavioral assessment of the CFA model rats (n = 6). There was no significant difference in 50% PWT between the ipsilateral and contralateral PWT of the sham group or between the contralateral sides of the CFA and sham groups.Notes: The mechanical sensitivity of the hind paw was determined as the 50% PWT. Data are mean ± SD, *P < 0.05 (A) vs the contralateral PWT of the SNL model; (B) vs the contralateral PWT of the CFA model rat.Abbreviations: L, lumbar; SNL, spinal nerve ligation; PWT, paw withdrawal threshold; CFA, complete Freund’s adjuvant; SD, standard deviation; Ipsi, ipsilateral side; Contra, contralateral side.
© Copyright Policy
Related In: Results  -  Collection

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f1-jpr-6-087: (A) Behavioral assessment of the L5 spinal nerve ligation model (n = 7). There was no significant difference in 50% PWT between the ipsilateral and contralateral PWT of the sham group or between the contralateral sides of the SNL and sham groups; (B) behavioral assessment of the CFA model rats (n = 6). There was no significant difference in 50% PWT between the ipsilateral and contralateral PWT of the sham group or between the contralateral sides of the CFA and sham groups.Notes: The mechanical sensitivity of the hind paw was determined as the 50% PWT. Data are mean ± SD, *P < 0.05 (A) vs the contralateral PWT of the SNL model; (B) vs the contralateral PWT of the CFA model rat.Abbreviations: L, lumbar; SNL, spinal nerve ligation; PWT, paw withdrawal threshold; CFA, complete Freund’s adjuvant; SD, standard deviation; Ipsi, ipsilateral side; Contra, contralateral side.
Mentions: L5SNL model rats are well-established as a neuropathic pain model that shows hyperalgesia, without phenomena such as paralysis, when spinal nerve ligation/injury is successfully induced.12 To investigate hyperalgesia in L5SNL rats, we performed behavioral assessments with the von-Frey test before and after L5SNL. We used a CFA model, which shows hyperalgesia as a non-nerve injury.13 Mechanical allodynia was observed in L5SNL model rats throughout the examination period (days 1–14 after L5SNL). The CFA injection induced allodynia at 3 days after treatment. Prior to SNL and intraplantar injection of CFA, there were no differences in 50% PWT on the bilateral hind paws (ipsilateral 13.6 ± 1.8 g vs contralateral 13.6 ± 1.8 g; ipsilateral 15.0 ± 0 g vs contralateral 15.0 ± 0 g, respectively). The 50% PWTs in the SNL group were 3.1 ± 0.8, 2.8 ± 0.9, 2.5 ± 1.4, 3.2 ± 1.2, 2.1 ± 0.4, and 2.5 ± 1.0 g at 1, 3, 7, 9, 11, and 14 days after surgery, respectively (Figure 1A). Significantly decreased 50% PWTs were observed from days 1 through 14 after surgery. In the CFA group, the ipsilateral 50% PWT was also significantly lower on days 1 and 3 (2.8 ± 1.8 g and 4.2 ± 3.8 g, respectively) (Figure 1B). There were no changes in the 50% PWT on the contralateral side in any of all the groups. There were also no changes in 50% PWT on either the ipsilateral or contralateral sides in the sham and naïve groups. The model rats were thus validated based on these results.

Bottom Line: It is generally known that peripheral nerve injury causes changes in expression of some growth factors in the dorsal root ganglion.The expression of ErbB2 and ErbB3 was observed in the dorsal root ganglia of the mature rat, despite ErbB1 and ErbB4 showing only subtle expression.We also demonstrated that peripheral nerve injury induced significant increases in ErbB2 and ErbB3 in the ipsilateral dorsal root ganglion as compared with uninjured nerve.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology and Resuscitology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.

ABSTRACT
It is generally known that peripheral nerve injury causes changes in expression of some growth factors in the dorsal root ganglion. Altered expression of ErbB receptors, a well-known growth factor in somatic cells, reportedly follows peripheral nerve injury in the spinal dorsal horn; however, it remains unknown whether the expression of these receptors is altered in the dorsal root ganglion after nerve injury. Therefore, this study examined the gene expression profiles of ErbB receptors in bilateral lumbar (L)4/L5 dorsal root ganglia, using L5-selective spinal nerve ligation in model rats as a peripheral nerve injury model. The expression of ErbB2 and ErbB3 was observed in the dorsal root ganglia of the mature rat, despite ErbB1 and ErbB4 showing only subtle expression. We also demonstrated that peripheral nerve injury induced significant increases in ErbB2 and ErbB3 in the ipsilateral dorsal root ganglion as compared with uninjured nerve. Expression changes in ErbB receptors appear to play important roles in nerve injury and subsequent nerve regeneration.

No MeSH data available.


Related in: MedlinePlus