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N-cadherin is differentially expressed in histological subtypes of papillary renal cell carcinoma.

Behnes CL, Hemmerlein B, Strauss A, Radzun HJ, Bremmer F - Diagn Pathol (2012)

Bottom Line: All papillary RCC type II displayed a membranous expression for N-cadherin, whereas type I did not show any membranous positivity for N-cadherin.E-cadherin exhibited a stronger, but not significant, membranous as well as cytoplasmic expression in type II than in type I papillary RCC.Thus N-cadherin represents the first immunhistochemical marker for a clear cut differentiation between papillary RCC type I and type II and could be a target for therapy and diagnostic in the future.

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Affiliation: Department of Pathology, University of Göttingen, Robert-Koch-Str 40, 37083 Göttingen, Germany. clbehnes@med.uni-goettingen.de

ABSTRACT

Background: Papillary renal cell carcinoma (RCC) represents a rare tumor, which is divided, based on histological criteria, into two subtypes. In contrast to type I papillary RCC type II papillary RCC shows a worse prognosis. So far, reliable immunohistochemical markers for the distinction of these subtypes are not available.

Methods: In the present study the expression of N(neural)-, E(epithelial)-, P(placental)-, and KSP(kidney specific)-cadherin was examined in 22 papillary RCC of histological type I and 18 papillary RCC of histological type II (n = 40).

Results: All papillary RCC type II displayed a membranous expression for N-cadherin, whereas type I did not show any membranous positivity for N-cadherin. E-cadherin exhibited a stronger, but not significant, membranous as well as cytoplasmic expression in type II than in type I papillary RCC. A diagnostic relevant expression of P- and KSP-cadherin could not be demonstrated in both tumor entities.

Conclusion: Thus N-cadherin represents the first immunhistochemical marker for a clear cut differentiation between papillary RCC type I and type II and could be a target for therapy and diagnostic in the future.

Virtual slides: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/2011556982761733.

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Immunohistochemistry of N-cadherin expression in papillary RCC. Papillary RCC type II with high nuclear polymorphism and abundant cytoplasm of the tumor cells (A, x20) displays a strong membranous expression of N-cadherin (B, x40). Papillary RCC type I with a single layer of cuboidal cells and a small cytoplasmic rim (C, x20) shows only a weak cytoplasmic N-cadherin (D, x40).
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Figure 1: Immunohistochemistry of N-cadherin expression in papillary RCC. Papillary RCC type II with high nuclear polymorphism and abundant cytoplasm of the tumor cells (A, x20) displays a strong membranous expression of N-cadherin (B, x40). Papillary RCC type I with a single layer of cuboidal cells and a small cytoplasmic rim (C, x20) shows only a weak cytoplasmic N-cadherin (D, x40).

Mentions: Immunohistochemical examinations revealed a membranous positivity for N-cadherin in all papillary RCC type II (IRS 6.28 ± 1.57), whereas type I did not show any membranous positivity for N-cadherin (IRS 0). In contrast to the membranous N-cadherin expression the cytoplasmic expression of N-cadherin showed a higher score in papillary RCC type I (IRS 5.68 ± 1.76) than in type II (IRS 2.5 ± 2.0); cytoplasmic N-cadherin was particularly detectable in the apical and basolateral parts of the tumorcells. The comparison between both subtypes of papillary RCC for cytoplasmic N-cadherin showed a significant difference (P < 0.0032) (Figure 1A-D and Figure 2).


N-cadherin is differentially expressed in histological subtypes of papillary renal cell carcinoma.

Behnes CL, Hemmerlein B, Strauss A, Radzun HJ, Bremmer F - Diagn Pathol (2012)

Immunohistochemistry of N-cadherin expression in papillary RCC. Papillary RCC type II with high nuclear polymorphism and abundant cytoplasm of the tumor cells (A, x20) displays a strong membranous expression of N-cadherin (B, x40). Papillary RCC type I with a single layer of cuboidal cells and a small cytoplasmic rim (C, x20) shows only a weak cytoplasmic N-cadherin (D, x40).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3539962&req=5

Figure 1: Immunohistochemistry of N-cadherin expression in papillary RCC. Papillary RCC type II with high nuclear polymorphism and abundant cytoplasm of the tumor cells (A, x20) displays a strong membranous expression of N-cadherin (B, x40). Papillary RCC type I with a single layer of cuboidal cells and a small cytoplasmic rim (C, x20) shows only a weak cytoplasmic N-cadherin (D, x40).
Mentions: Immunohistochemical examinations revealed a membranous positivity for N-cadherin in all papillary RCC type II (IRS 6.28 ± 1.57), whereas type I did not show any membranous positivity for N-cadherin (IRS 0). In contrast to the membranous N-cadherin expression the cytoplasmic expression of N-cadherin showed a higher score in papillary RCC type I (IRS 5.68 ± 1.76) than in type II (IRS 2.5 ± 2.0); cytoplasmic N-cadherin was particularly detectable in the apical and basolateral parts of the tumorcells. The comparison between both subtypes of papillary RCC for cytoplasmic N-cadherin showed a significant difference (P < 0.0032) (Figure 1A-D and Figure 2).

Bottom Line: All papillary RCC type II displayed a membranous expression for N-cadherin, whereas type I did not show any membranous positivity for N-cadherin.E-cadherin exhibited a stronger, but not significant, membranous as well as cytoplasmic expression in type II than in type I papillary RCC.Thus N-cadherin represents the first immunhistochemical marker for a clear cut differentiation between papillary RCC type I and type II and could be a target for therapy and diagnostic in the future.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pathology, University of Göttingen, Robert-Koch-Str 40, 37083 Göttingen, Germany. clbehnes@med.uni-goettingen.de

ABSTRACT

Background: Papillary renal cell carcinoma (RCC) represents a rare tumor, which is divided, based on histological criteria, into two subtypes. In contrast to type I papillary RCC type II papillary RCC shows a worse prognosis. So far, reliable immunohistochemical markers for the distinction of these subtypes are not available.

Methods: In the present study the expression of N(neural)-, E(epithelial)-, P(placental)-, and KSP(kidney specific)-cadherin was examined in 22 papillary RCC of histological type I and 18 papillary RCC of histological type II (n = 40).

Results: All papillary RCC type II displayed a membranous expression for N-cadherin, whereas type I did not show any membranous positivity for N-cadherin. E-cadherin exhibited a stronger, but not significant, membranous as well as cytoplasmic expression in type II than in type I papillary RCC. A diagnostic relevant expression of P- and KSP-cadherin could not be demonstrated in both tumor entities.

Conclusion: Thus N-cadherin represents the first immunhistochemical marker for a clear cut differentiation between papillary RCC type I and type II and could be a target for therapy and diagnostic in the future.

Virtual slides: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/2011556982761733.

Show MeSH
Related in: MedlinePlus