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An antibody microarray analysis of serum cytokines in neurodegenerative Parkinsonian syndromes.

Mahlknecht P, Stemberger S, Sprenger F, Rainer J, Hametner E, Kirchmair R, Grabmer C, Scherfler C, Wenning GK, Seppi K, Poewe W, Reindl M - Proteome Sci (2012)

Bottom Line: In a next step, results from the microarray experiment were individually validated by different immunoassays.Immunoassay validation confirmed a significant increase of median PDGF-BB levels in patients with PSP/CBS, MSA and PD and a decrease of median prolactin levels in PD.However, neither PDGF-BB nor prolactin were specific biomarkers to discriminate PSP/CBS, MSA, PD and controls.

View Article: PubMed Central - HTML - PubMed

Affiliation: Clinical Department of Neurology, Innsbruck Medical University, Innsbruck, Austria. Markus.Reindl@i-med.ac.at.

ABSTRACT

Background: Microarray technology may offer a new opportunity to gain insight into disease-specific global protein expression profiles. The present study was performed to apply a serum antibody microarray to screen for differentially regulated cytokines in Parkinson's disease (PD), multiple system atrophy (MSA), progressive supranuclear palsy (PSP) and corticobasal syndrome (CBS).

Results: Serum samples were obtained from patients with clinical diagnoses of PD (n = 117), MSA (n = 31) and PSP/CBS (n = 38) and 99 controls. Cytokine profiles of sera from patients and controls were analyzed with a semiquantitative human antibody array for 174 cytokines and the expression of 12 cytokines was found to be significantly altered. In a next step, results from the microarray experiment were individually validated by different immunoassays. Immunoassay validation confirmed a significant increase of median PDGF-BB levels in patients with PSP/CBS, MSA and PD and a decrease of median prolactin levels in PD. However, neither PDGF-BB nor prolactin were specific biomarkers to discriminate PSP/CBS, MSA, PD and controls.

Conclusions: In our unbiased cytokine array based screening approach and validation by a different immunoassay only two of 174 cytokines were significantly altered between patients and controls.

No MeSH data available.


Related in: MedlinePlus

Differential expression of PDGF-BB and prolactin in patients with PSP/CBS, MSA and controls in the screening and replication cohorts. Individual data points are shown as circles and horizontal bars indicate medians. In addition data are shown as box plot with medians indicated as horizontal bars with boxes. Groups were compared using the Kruskal-Wallis test and Dunn’s multiple comparison post-hoc test and overall p-values are shown in each figure. * Significant differences to the control group, # significant differences to PD patients.
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Figure 3: Differential expression of PDGF-BB and prolactin in patients with PSP/CBS, MSA and controls in the screening and replication cohorts. Individual data points are shown as circles and horizontal bars indicate medians. In addition data are shown as box plot with medians indicated as horizontal bars with boxes. Groups were compared using the Kruskal-Wallis test and Dunn’s multiple comparison post-hoc test and overall p-values are shown in each figure. * Significant differences to the control group, # significant differences to PD patients.

Mentions: In a next step we extended the analysis of PDGF-BB and prolactin to a replication cohort of patients and controls (Table 1B) and to all patients and controls. Figures 2 and 3 demonstrate that these two cytokines were significantly different among the four groups in the screening and replication cohorts and in the combined data. PDGF-BB was significantly increased in PSP/CBS, MSA and PD and prolactin was significantly decreased in PD (Figure 3, Table 4). From Table 4 it is also evident that PDGF-BB levels were mainly influenced by the clinical diagnosis, whereas prolactin levels were more strongly influenced by antiparkinson treatment (p = 7x10-13) than by clinical diagnosis (p = 7x10-7).


An antibody microarray analysis of serum cytokines in neurodegenerative Parkinsonian syndromes.

Mahlknecht P, Stemberger S, Sprenger F, Rainer J, Hametner E, Kirchmair R, Grabmer C, Scherfler C, Wenning GK, Seppi K, Poewe W, Reindl M - Proteome Sci (2012)

Differential expression of PDGF-BB and prolactin in patients with PSP/CBS, MSA and controls in the screening and replication cohorts. Individual data points are shown as circles and horizontal bars indicate medians. In addition data are shown as box plot with medians indicated as horizontal bars with boxes. Groups were compared using the Kruskal-Wallis test and Dunn’s multiple comparison post-hoc test and overall p-values are shown in each figure. * Significant differences to the control group, # significant differences to PD patients.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3539904&req=5

Figure 3: Differential expression of PDGF-BB and prolactin in patients with PSP/CBS, MSA and controls in the screening and replication cohorts. Individual data points are shown as circles and horizontal bars indicate medians. In addition data are shown as box plot with medians indicated as horizontal bars with boxes. Groups were compared using the Kruskal-Wallis test and Dunn’s multiple comparison post-hoc test and overall p-values are shown in each figure. * Significant differences to the control group, # significant differences to PD patients.
Mentions: In a next step we extended the analysis of PDGF-BB and prolactin to a replication cohort of patients and controls (Table 1B) and to all patients and controls. Figures 2 and 3 demonstrate that these two cytokines were significantly different among the four groups in the screening and replication cohorts and in the combined data. PDGF-BB was significantly increased in PSP/CBS, MSA and PD and prolactin was significantly decreased in PD (Figure 3, Table 4). From Table 4 it is also evident that PDGF-BB levels were mainly influenced by the clinical diagnosis, whereas prolactin levels were more strongly influenced by antiparkinson treatment (p = 7x10-13) than by clinical diagnosis (p = 7x10-7).

Bottom Line: In a next step, results from the microarray experiment were individually validated by different immunoassays.Immunoassay validation confirmed a significant increase of median PDGF-BB levels in patients with PSP/CBS, MSA and PD and a decrease of median prolactin levels in PD.However, neither PDGF-BB nor prolactin were specific biomarkers to discriminate PSP/CBS, MSA, PD and controls.

View Article: PubMed Central - HTML - PubMed

Affiliation: Clinical Department of Neurology, Innsbruck Medical University, Innsbruck, Austria. Markus.Reindl@i-med.ac.at.

ABSTRACT

Background: Microarray technology may offer a new opportunity to gain insight into disease-specific global protein expression profiles. The present study was performed to apply a serum antibody microarray to screen for differentially regulated cytokines in Parkinson's disease (PD), multiple system atrophy (MSA), progressive supranuclear palsy (PSP) and corticobasal syndrome (CBS).

Results: Serum samples were obtained from patients with clinical diagnoses of PD (n = 117), MSA (n = 31) and PSP/CBS (n = 38) and 99 controls. Cytokine profiles of sera from patients and controls were analyzed with a semiquantitative human antibody array for 174 cytokines and the expression of 12 cytokines was found to be significantly altered. In a next step, results from the microarray experiment were individually validated by different immunoassays. Immunoassay validation confirmed a significant increase of median PDGF-BB levels in patients with PSP/CBS, MSA and PD and a decrease of median prolactin levels in PD. However, neither PDGF-BB nor prolactin were specific biomarkers to discriminate PSP/CBS, MSA, PD and controls.

Conclusions: In our unbiased cytokine array based screening approach and validation by a different immunoassay only two of 174 cytokines were significantly altered between patients and controls.

No MeSH data available.


Related in: MedlinePlus