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Exercise training performed simultaneously to a high-fat diet reduces the degree of insulin resistance and improves adipoR1-2/APPL1 protein levels in mice.

Farias JM, Maggi RM, Tromm CB, Silva LA, Luciano TF, Marques SO, Lira FS, de Souza CT, Pinho RA - Lipids Health Dis (2012)

Bottom Line: IR, IRs, and Akt phosphorylation decreased in the DIO group in the three analyzed tissues.In addition, the DIO group exhibited ADIPOR1 (skeletal muscle and adipose tissue), ADIPOR2 (liver), and APPL1 reduced when compared with the C group.In parallel, ADIPOR1 and 2 and APPL1 protein levels significantly increase in exercised mice.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratory of Exercise Biochemistry and Physiology, Postgraduate Program in Health Sciences, Health Sciences Unit, Universidade do Extremo Sul Catarinense, Criciúma, SC, Brazil.

ABSTRACT

Background: The aim of the present study was to evaluate the protective effect of concurrent exercise in the degree of the insulin resistance in mice fed with a high-fat diet, and assess adiponectin receptors (ADIPOR1 and ADIPOR2) and endosomal adaptor protein APPL1 in different tissues.

Methods: Twenty-four mice were randomized into four groups (n = 6): chow standard diet and sedentary (C); chow standard diet and simultaneous exercise training (C-T); fed on a high-fat diet and sedentary (DIO); and fed on a high-fat diet and simultaneous exercise training (DIO-T). Simultaneously to starting high-fat diet feeding, the mice were submitted to a swimming exercise training protocol (2 x 30 minutes, with 5 minutes of interval/day), five days per week, for twelve weeks (90 days). Animals were then euthanized 48 hours after the last exercise training session, and adipose, liver, and skeletal muscle tissue were extracted for an immunoblotting analysis.

Results: IR, IRs, and Akt phosphorylation decreased in the DIO group in the three analyzed tissues. In addition, the DIO group exhibited ADIPOR1 (skeletal muscle and adipose tissue), ADIPOR2 (liver), and APPL1 reduced when compared with the C group. However, it was reverted when exercise training was simultaneously performed. In parallel, ADIPOR1 and 2 and APPL1 protein levels significantly increase in exercised mice.

Conclusions: Our findings demonstrate that exercise training performed concomitantly to a high-fat diet reduces the degree of insulin resistance and improves adipoR1-2/APPL1 protein levels in the hepatic, adipose, and skeletal muscle tissue.

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Related in: MedlinePlus

Effects of exercise training concomitant to high-fat diet feeding on IR, IRS1, and Akt phosphorylation and adipoR1/APPL1 protein levels in the adipose tissue. Analysis of insulin-induced tyrosine phosphorylation of IR (A), IRS1 (B), phosphorylation of AktSer473 (C) and ADIPOR1 (D) and APPL1 (E) protein levels. In lower panels, original membrane stripped and reblotted for IR, IRS1,and Akt total protein levels (A, B and C, respectively) and β-actin (D and E). The results are presented as means ± SEM of n = 6, *p < 0.05, different from C-T and C groups with insulin injection versus C-T and C groups with saline injection, #p < 0.05 different from DIO versus control groups, and $p < 0.05 different from DIO-T versus DIO groups.
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Figure 1: Effects of exercise training concomitant to high-fat diet feeding on IR, IRS1, and Akt phosphorylation and adipoR1/APPL1 protein levels in the adipose tissue. Analysis of insulin-induced tyrosine phosphorylation of IR (A), IRS1 (B), phosphorylation of AktSer473 (C) and ADIPOR1 (D) and APPL1 (E) protein levels. In lower panels, original membrane stripped and reblotted for IR, IRS1,and Akt total protein levels (A, B and C, respectively) and β-actin (D and E). The results are presented as means ± SEM of n = 6, *p < 0.05, different from C-T and C groups with insulin injection versus C-T and C groups with saline injection, #p < 0.05 different from DIO versus control groups, and $p < 0.05 different from DIO-T versus DIO groups.

Mentions: The effects of exercise training on levels of phosphorylation of IR, IRS1, and Akt were examined in the adipose tissue of C, C-T, DIO, and DIO-T groups submitted to the exercise training. As expected, an insulin (+) injection showed increased IR, IRS, and Akt phosphorylation (Figures1A,1B, and1C, respectively) in the adipose tissue of mice that were fed chow standard (sedentary and training groups) when compared with those that were administered a saline (−) injection. However, when mice were fed with a high-fat diet and not submitted to training protocol (DIO group), IR, IRS, and Akt phosphorylation (Figures1A,1B, and1C, respectively) in adipose tissue were reduced, when compared with control group. On the other hand, IR, IRS, and Akt phosphorylation (Figure1A, B, and C, respectively) in the adipose tissue of the DIO + T group increased, when compared with the DIO sedentary group. In parallel, the adiponectin receptor 1 (ADIPOR1) and its molecule adaptor APPL1 exhibit market levels reduced when compared with control sedentary and training groups (Figure1D and1E, respectively). However, when exercise training was performed simultaneously to high-fat diet feeding, it was partially reverted (Figure1D and E, respectively).


Exercise training performed simultaneously to a high-fat diet reduces the degree of insulin resistance and improves adipoR1-2/APPL1 protein levels in mice.

Farias JM, Maggi RM, Tromm CB, Silva LA, Luciano TF, Marques SO, Lira FS, de Souza CT, Pinho RA - Lipids Health Dis (2012)

Effects of exercise training concomitant to high-fat diet feeding on IR, IRS1, and Akt phosphorylation and adipoR1/APPL1 protein levels in the adipose tissue. Analysis of insulin-induced tyrosine phosphorylation of IR (A), IRS1 (B), phosphorylation of AktSer473 (C) and ADIPOR1 (D) and APPL1 (E) protein levels. In lower panels, original membrane stripped and reblotted for IR, IRS1,and Akt total protein levels (A, B and C, respectively) and β-actin (D and E). The results are presented as means ± SEM of n = 6, *p < 0.05, different from C-T and C groups with insulin injection versus C-T and C groups with saline injection, #p < 0.05 different from DIO versus control groups, and $p < 0.05 different from DIO-T versus DIO groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3539884&req=5

Figure 1: Effects of exercise training concomitant to high-fat diet feeding on IR, IRS1, and Akt phosphorylation and adipoR1/APPL1 protein levels in the adipose tissue. Analysis of insulin-induced tyrosine phosphorylation of IR (A), IRS1 (B), phosphorylation of AktSer473 (C) and ADIPOR1 (D) and APPL1 (E) protein levels. In lower panels, original membrane stripped and reblotted for IR, IRS1,and Akt total protein levels (A, B and C, respectively) and β-actin (D and E). The results are presented as means ± SEM of n = 6, *p < 0.05, different from C-T and C groups with insulin injection versus C-T and C groups with saline injection, #p < 0.05 different from DIO versus control groups, and $p < 0.05 different from DIO-T versus DIO groups.
Mentions: The effects of exercise training on levels of phosphorylation of IR, IRS1, and Akt were examined in the adipose tissue of C, C-T, DIO, and DIO-T groups submitted to the exercise training. As expected, an insulin (+) injection showed increased IR, IRS, and Akt phosphorylation (Figures1A,1B, and1C, respectively) in the adipose tissue of mice that were fed chow standard (sedentary and training groups) when compared with those that were administered a saline (−) injection. However, when mice were fed with a high-fat diet and not submitted to training protocol (DIO group), IR, IRS, and Akt phosphorylation (Figures1A,1B, and1C, respectively) in adipose tissue were reduced, when compared with control group. On the other hand, IR, IRS, and Akt phosphorylation (Figure1A, B, and C, respectively) in the adipose tissue of the DIO + T group increased, when compared with the DIO sedentary group. In parallel, the adiponectin receptor 1 (ADIPOR1) and its molecule adaptor APPL1 exhibit market levels reduced when compared with control sedentary and training groups (Figure1D and1E, respectively). However, when exercise training was performed simultaneously to high-fat diet feeding, it was partially reverted (Figure1D and E, respectively).

Bottom Line: IR, IRs, and Akt phosphorylation decreased in the DIO group in the three analyzed tissues.In addition, the DIO group exhibited ADIPOR1 (skeletal muscle and adipose tissue), ADIPOR2 (liver), and APPL1 reduced when compared with the C group.In parallel, ADIPOR1 and 2 and APPL1 protein levels significantly increase in exercised mice.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratory of Exercise Biochemistry and Physiology, Postgraduate Program in Health Sciences, Health Sciences Unit, Universidade do Extremo Sul Catarinense, Criciúma, SC, Brazil.

ABSTRACT

Background: The aim of the present study was to evaluate the protective effect of concurrent exercise in the degree of the insulin resistance in mice fed with a high-fat diet, and assess adiponectin receptors (ADIPOR1 and ADIPOR2) and endosomal adaptor protein APPL1 in different tissues.

Methods: Twenty-four mice were randomized into four groups (n = 6): chow standard diet and sedentary (C); chow standard diet and simultaneous exercise training (C-T); fed on a high-fat diet and sedentary (DIO); and fed on a high-fat diet and simultaneous exercise training (DIO-T). Simultaneously to starting high-fat diet feeding, the mice were submitted to a swimming exercise training protocol (2 x 30 minutes, with 5 minutes of interval/day), five days per week, for twelve weeks (90 days). Animals were then euthanized 48 hours after the last exercise training session, and adipose, liver, and skeletal muscle tissue were extracted for an immunoblotting analysis.

Results: IR, IRs, and Akt phosphorylation decreased in the DIO group in the three analyzed tissues. In addition, the DIO group exhibited ADIPOR1 (skeletal muscle and adipose tissue), ADIPOR2 (liver), and APPL1 reduced when compared with the C group. However, it was reverted when exercise training was simultaneously performed. In parallel, ADIPOR1 and 2 and APPL1 protein levels significantly increase in exercised mice.

Conclusions: Our findings demonstrate that exercise training performed concomitantly to a high-fat diet reduces the degree of insulin resistance and improves adipoR1-2/APPL1 protein levels in the hepatic, adipose, and skeletal muscle tissue.

Show MeSH
Related in: MedlinePlus