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Expression of MMP-2 and TIMP-1 in renal tissue of patients with chronic active antibody-mediated renal graft rejection.

Yan Q, Sui W, Wang B, Zou H, Zou G, Luo H - Diagn Pathol (2012)

Bottom Line: MMP-2 expression tended to decrease, while TIMP-1 and serum creatinine increased along with the increase of pathological grade of IF/TA (P < 0.05).In IF/TA groups, the expression of TIMP-1 was positively correlated to serum creatinine level (r = 0.718, P < 0.05).It is suggested by the results that abnormal expressions of MMP-2 and TIMP-1 might play roles in the development of renal fibrosis in chronic AMR.

View Article: PubMed Central - HTML - PubMed

Affiliation: Dialysis and Kidney Transplantation Center of PLA, 181 Hospital of PLA, Guilin 541002, China.

ABSTRACT

Objective: To investigate the expression of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of metallopropteinase-1 (TIMP-1) in the renal allografts of patients with chronic active antibody-mediated rejection (AMR), and to explore their role in the pathogenesis of AMR.

Methods: Immunohistochemistry assay and computer-assisted image analysis were used to detect the expression of MMP-2 and TIMP-1 in the renal allografts with interstitial fibrosis and tubular atrophy (IF/TA) in 46 transplant recipients and 15 normal renal tissue specimens as the controls. The association of the expression level of either MMP-2 or TIMP-1 with the pathological grade of IF/TA in AMR was analyzed.

Results: The expression of either MMP-2 or TIMP-1 was significantly increased in the renal allografts of the recipients as compared with the normal renal tissue (P < 0.05). MMP-2 expression tended to decrease, while TIMP-1 and serum creatinine increased along with the increase of pathological grade of IF/TA (P < 0.05). In IF/TA groups, the expression of TIMP-1 was positively correlated to serum creatinine level (r = 0.718, P < 0.05).

Conclusions: It is suggested by the results that abnormal expressions of MMP-2 and TIMP-1 might play roles in the development of renal fibrosis in chronic AMR.

Virtual slides: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1128474926172838.

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TIMP-1 expressions in renal tissue with different degree of interstitial fibrosis/tubular atrophy. (EnVision assay; original magnification × 200). IF/TA-I Group.
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Figure 6: TIMP-1 expressions in renal tissue with different degree of interstitial fibrosis/tubular atrophy. (EnVision assay; original magnification × 200). IF/TA-I Group.

Mentions: MMP-2 and TIMP-1 were mainly expressed in renal tubular epithelial cells, renal tubular basement membrane and cytoplasm of interstitial cells, looking like pale yellow, pale brown and russet particals as positive. There was no or rare expression of MMP-2 and TIMP-1 in normal kidney tissue, but there was significant expression of MMP-2 and TIMP-1 in AMR groups, mainly in tubular epithelial cells and cytoplasm of interstitial cells. MMP-2 was expressed to the most in IF-TA group, but decreased with the aggravation of mesenchyme disorder. However the expression of TIMP-1 increased with the aggravation of mesenchyme disease (Figures1,2,3,4,5,6,7,8). Expression of MMP-2 and TIMP-1 in tubular interstitial cell increased significantly in AMR groups compared to normal group, there was significant difference (P < 0.001), and expression of MMP-2 was decreaing with the increase of IF/TA pathological classification (P < 0.05), expression of TIMP-1 was increaing with the increase of IF/TA pathological classification (P < 0.05, Table1).


Expression of MMP-2 and TIMP-1 in renal tissue of patients with chronic active antibody-mediated renal graft rejection.

Yan Q, Sui W, Wang B, Zou H, Zou G, Luo H - Diagn Pathol (2012)

TIMP-1 expressions in renal tissue with different degree of interstitial fibrosis/tubular atrophy. (EnVision assay; original magnification × 200). IF/TA-I Group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3539883&req=5

Figure 6: TIMP-1 expressions in renal tissue with different degree of interstitial fibrosis/tubular atrophy. (EnVision assay; original magnification × 200). IF/TA-I Group.
Mentions: MMP-2 and TIMP-1 were mainly expressed in renal tubular epithelial cells, renal tubular basement membrane and cytoplasm of interstitial cells, looking like pale yellow, pale brown and russet particals as positive. There was no or rare expression of MMP-2 and TIMP-1 in normal kidney tissue, but there was significant expression of MMP-2 and TIMP-1 in AMR groups, mainly in tubular epithelial cells and cytoplasm of interstitial cells. MMP-2 was expressed to the most in IF-TA group, but decreased with the aggravation of mesenchyme disorder. However the expression of TIMP-1 increased with the aggravation of mesenchyme disease (Figures1,2,3,4,5,6,7,8). Expression of MMP-2 and TIMP-1 in tubular interstitial cell increased significantly in AMR groups compared to normal group, there was significant difference (P < 0.001), and expression of MMP-2 was decreaing with the increase of IF/TA pathological classification (P < 0.05), expression of TIMP-1 was increaing with the increase of IF/TA pathological classification (P < 0.05, Table1).

Bottom Line: MMP-2 expression tended to decrease, while TIMP-1 and serum creatinine increased along with the increase of pathological grade of IF/TA (P < 0.05).In IF/TA groups, the expression of TIMP-1 was positively correlated to serum creatinine level (r = 0.718, P < 0.05).It is suggested by the results that abnormal expressions of MMP-2 and TIMP-1 might play roles in the development of renal fibrosis in chronic AMR.

View Article: PubMed Central - HTML - PubMed

Affiliation: Dialysis and Kidney Transplantation Center of PLA, 181 Hospital of PLA, Guilin 541002, China.

ABSTRACT

Objective: To investigate the expression of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of metallopropteinase-1 (TIMP-1) in the renal allografts of patients with chronic active antibody-mediated rejection (AMR), and to explore their role in the pathogenesis of AMR.

Methods: Immunohistochemistry assay and computer-assisted image analysis were used to detect the expression of MMP-2 and TIMP-1 in the renal allografts with interstitial fibrosis and tubular atrophy (IF/TA) in 46 transplant recipients and 15 normal renal tissue specimens as the controls. The association of the expression level of either MMP-2 or TIMP-1 with the pathological grade of IF/TA in AMR was analyzed.

Results: The expression of either MMP-2 or TIMP-1 was significantly increased in the renal allografts of the recipients as compared with the normal renal tissue (P < 0.05). MMP-2 expression tended to decrease, while TIMP-1 and serum creatinine increased along with the increase of pathological grade of IF/TA (P < 0.05). In IF/TA groups, the expression of TIMP-1 was positively correlated to serum creatinine level (r = 0.718, P < 0.05).

Conclusions: It is suggested by the results that abnormal expressions of MMP-2 and TIMP-1 might play roles in the development of renal fibrosis in chronic AMR.

Virtual slides: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1128474926172838.

Show MeSH
Related in: MedlinePlus