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Expression of MMP-2 and TIMP-1 in renal tissue of patients with chronic active antibody-mediated renal graft rejection.

Yan Q, Sui W, Wang B, Zou H, Zou G, Luo H - Diagn Pathol (2012)

Bottom Line: MMP-2 expression tended to decrease, while TIMP-1 and serum creatinine increased along with the increase of pathological grade of IF/TA (P < 0.05).In IF/TA groups, the expression of TIMP-1 was positively correlated to serum creatinine level (r = 0.718, P < 0.05).It is suggested by the results that abnormal expressions of MMP-2 and TIMP-1 might play roles in the development of renal fibrosis in chronic AMR.

View Article: PubMed Central - HTML - PubMed

Affiliation: Dialysis and Kidney Transplantation Center of PLA, 181 Hospital of PLA, Guilin 541002, China.

ABSTRACT

Objective: To investigate the expression of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of metallopropteinase-1 (TIMP-1) in the renal allografts of patients with chronic active antibody-mediated rejection (AMR), and to explore their role in the pathogenesis of AMR.

Methods: Immunohistochemistry assay and computer-assisted image analysis were used to detect the expression of MMP-2 and TIMP-1 in the renal allografts with interstitial fibrosis and tubular atrophy (IF/TA) in 46 transplant recipients and 15 normal renal tissue specimens as the controls. The association of the expression level of either MMP-2 or TIMP-1 with the pathological grade of IF/TA in AMR was analyzed.

Results: The expression of either MMP-2 or TIMP-1 was significantly increased in the renal allografts of the recipients as compared with the normal renal tissue (P < 0.05). MMP-2 expression tended to decrease, while TIMP-1 and serum creatinine increased along with the increase of pathological grade of IF/TA (P < 0.05). In IF/TA groups, the expression of TIMP-1 was positively correlated to serum creatinine level (r = 0.718, P < 0.05).

Conclusions: It is suggested by the results that abnormal expressions of MMP-2 and TIMP-1 might play roles in the development of renal fibrosis in chronic AMR.

Virtual slides: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1128474926172838.

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Expression of C4d and renal histology. Glomerular double contours (original magnification × 400).
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Figure 11: Expression of C4d and renal histology. Glomerular double contours (original magnification × 400).

Mentions: Rare C4d deposits were found in endothelial cell and/or basal membrane of peri-tubular capillaries in normal kidney tissue, however diffuse and brightening positive linear deposits of C4d of 50% peri-tubular capillaries in AMR. The deposit of C4d in 50% peri-tubular capillaries endothelial cells and basal membrane was significant morphological dis- orders of complement activation in AMR (Figures9,10,11).


Expression of MMP-2 and TIMP-1 in renal tissue of patients with chronic active antibody-mediated renal graft rejection.

Yan Q, Sui W, Wang B, Zou H, Zou G, Luo H - Diagn Pathol (2012)

Expression of C4d and renal histology. Glomerular double contours (original magnification × 400).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3539883&req=5

Figure 11: Expression of C4d and renal histology. Glomerular double contours (original magnification × 400).
Mentions: Rare C4d deposits were found in endothelial cell and/or basal membrane of peri-tubular capillaries in normal kidney tissue, however diffuse and brightening positive linear deposits of C4d of 50% peri-tubular capillaries in AMR. The deposit of C4d in 50% peri-tubular capillaries endothelial cells and basal membrane was significant morphological dis- orders of complement activation in AMR (Figures9,10,11).

Bottom Line: MMP-2 expression tended to decrease, while TIMP-1 and serum creatinine increased along with the increase of pathological grade of IF/TA (P < 0.05).In IF/TA groups, the expression of TIMP-1 was positively correlated to serum creatinine level (r = 0.718, P < 0.05).It is suggested by the results that abnormal expressions of MMP-2 and TIMP-1 might play roles in the development of renal fibrosis in chronic AMR.

View Article: PubMed Central - HTML - PubMed

Affiliation: Dialysis and Kidney Transplantation Center of PLA, 181 Hospital of PLA, Guilin 541002, China.

ABSTRACT

Objective: To investigate the expression of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of metallopropteinase-1 (TIMP-1) in the renal allografts of patients with chronic active antibody-mediated rejection (AMR), and to explore their role in the pathogenesis of AMR.

Methods: Immunohistochemistry assay and computer-assisted image analysis were used to detect the expression of MMP-2 and TIMP-1 in the renal allografts with interstitial fibrosis and tubular atrophy (IF/TA) in 46 transplant recipients and 15 normal renal tissue specimens as the controls. The association of the expression level of either MMP-2 or TIMP-1 with the pathological grade of IF/TA in AMR was analyzed.

Results: The expression of either MMP-2 or TIMP-1 was significantly increased in the renal allografts of the recipients as compared with the normal renal tissue (P < 0.05). MMP-2 expression tended to decrease, while TIMP-1 and serum creatinine increased along with the increase of pathological grade of IF/TA (P < 0.05). In IF/TA groups, the expression of TIMP-1 was positively correlated to serum creatinine level (r = 0.718, P < 0.05).

Conclusions: It is suggested by the results that abnormal expressions of MMP-2 and TIMP-1 might play roles in the development of renal fibrosis in chronic AMR.

Virtual slides: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1128474926172838.

Show MeSH
Related in: MedlinePlus