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Isolation and characterization of a resident tolerant Saccharomyces cerevisiae strain from a spent sulfite liquor fermentation plant.

Sànchez I Nogué V, Bettiga M, Gorwa-Grauslund MF - AMB Express (2012)

Bottom Line: Spent Sulfite Liquor (SSL) from wood pulping facilities is a sugar rich effluent that can be used as feedstock for ethanol production.During fermentation of undiluted SSL, TMB3720 displayed a 4-fold higher ethanol production rate and 1.8-fold higher ethanol yield as compared to the commercial baker's yeast.Another non-Saccharomyces cerevisiae species, identified as the pentose utilizing Pichia galeiformis, was also recovered in the last tanks of the process where the hexose to pentose sugar ratio and the inhibitory pressure are expected to be the lowest.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Applied Microbiology, Lund University, P,O, Box 124, SE-221 00, Lund, Sweden. marie-francoise.gorwa@tmb.lth.se.

ABSTRACT
Spent Sulfite Liquor (SSL) from wood pulping facilities is a sugar rich effluent that can be used as feedstock for ethanol production. However, depending on the pulping process conditions, the release of monosaccharides also generates a range of compounds that negatively affect microbial fermentation. In the present study, we investigated whether endogenous yeasts in SSL-based ethanol plant could represent a source of Saccharomyces cerevisiae strains with a naturally acquired tolerance towards this inhibitory environment. Two isolation processes were performed, before and after the re-inoculation of the plant with a commercial baker's yeast strain. The isolates were clustered by DNA fingerprinting and a recurrent Saccharomyces cerevisiae strain, different from the inoculated commercial baker's yeast strain, was isolated. The strain, named TMB3720, flocculated heavily and presented high furaldehyde reductase activity. During fermentation of undiluted SSL, TMB3720 displayed a 4-fold higher ethanol production rate and 1.8-fold higher ethanol yield as compared to the commercial baker's yeast. Another non-Saccharomyces cerevisiae species, identified as the pentose utilizing Pichia galeiformis, was also recovered in the last tanks of the process where the hexose to pentose sugar ratio and the inhibitory pressure are expected to be the lowest.

No MeSH data available.


Related in: MedlinePlus

Anaerobic ethanol production from undiluted SSL. TMB3720 (squares), BY (circles) and TMB3500 (triangles). Each grey is a representative profile (from 2 or more replicates) of the ethanol production for the tested isolates from the first (#1.3.14, #1.4.06), and the second isolation process (#2.1.48, #2.3.54 and #2.4.69).
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Figure 4: Anaerobic ethanol production from undiluted SSL. TMB3720 (squares), BY (circles) and TMB3500 (triangles). Each grey is a representative profile (from 2 or more replicates) of the ethanol production for the tested isolates from the first (#1.3.14, #1.4.06), and the second isolation process (#2.1.48, #2.3.54 and #2.4.69).

Mentions: Sugar consumption and ethanol production profiles were highly comparable for all selected isolates from both isolation processes (Figure 4). Also, for all tested isolates, no lag phase was observed and the maximum ethanol concentration was very similar (18.5 ± 0.6 g l-1, depending on the isolate). These data, in addition to the high similarity of the DNA fingerprints (85%) confirmed that the different isolates were originating from the same strain. Therefore, isolate #1.1.03 was chosen as representative for the comparison with BY and TMB3500 and was named TMB3720.


Isolation and characterization of a resident tolerant Saccharomyces cerevisiae strain from a spent sulfite liquor fermentation plant.

Sànchez I Nogué V, Bettiga M, Gorwa-Grauslund MF - AMB Express (2012)

Anaerobic ethanol production from undiluted SSL. TMB3720 (squares), BY (circles) and TMB3500 (triangles). Each grey is a representative profile (from 2 or more replicates) of the ethanol production for the tested isolates from the first (#1.3.14, #1.4.06), and the second isolation process (#2.1.48, #2.3.54 and #2.4.69).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3539867&req=5

Figure 4: Anaerobic ethanol production from undiluted SSL. TMB3720 (squares), BY (circles) and TMB3500 (triangles). Each grey is a representative profile (from 2 or more replicates) of the ethanol production for the tested isolates from the first (#1.3.14, #1.4.06), and the second isolation process (#2.1.48, #2.3.54 and #2.4.69).
Mentions: Sugar consumption and ethanol production profiles were highly comparable for all selected isolates from both isolation processes (Figure 4). Also, for all tested isolates, no lag phase was observed and the maximum ethanol concentration was very similar (18.5 ± 0.6 g l-1, depending on the isolate). These data, in addition to the high similarity of the DNA fingerprints (85%) confirmed that the different isolates were originating from the same strain. Therefore, isolate #1.1.03 was chosen as representative for the comparison with BY and TMB3500 and was named TMB3720.

Bottom Line: Spent Sulfite Liquor (SSL) from wood pulping facilities is a sugar rich effluent that can be used as feedstock for ethanol production.During fermentation of undiluted SSL, TMB3720 displayed a 4-fold higher ethanol production rate and 1.8-fold higher ethanol yield as compared to the commercial baker's yeast.Another non-Saccharomyces cerevisiae species, identified as the pentose utilizing Pichia galeiformis, was also recovered in the last tanks of the process where the hexose to pentose sugar ratio and the inhibitory pressure are expected to be the lowest.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Applied Microbiology, Lund University, P,O, Box 124, SE-221 00, Lund, Sweden. marie-francoise.gorwa@tmb.lth.se.

ABSTRACT
Spent Sulfite Liquor (SSL) from wood pulping facilities is a sugar rich effluent that can be used as feedstock for ethanol production. However, depending on the pulping process conditions, the release of monosaccharides also generates a range of compounds that negatively affect microbial fermentation. In the present study, we investigated whether endogenous yeasts in SSL-based ethanol plant could represent a source of Saccharomyces cerevisiae strains with a naturally acquired tolerance towards this inhibitory environment. Two isolation processes were performed, before and after the re-inoculation of the plant with a commercial baker's yeast strain. The isolates were clustered by DNA fingerprinting and a recurrent Saccharomyces cerevisiae strain, different from the inoculated commercial baker's yeast strain, was isolated. The strain, named TMB3720, flocculated heavily and presented high furaldehyde reductase activity. During fermentation of undiluted SSL, TMB3720 displayed a 4-fold higher ethanol production rate and 1.8-fold higher ethanol yield as compared to the commercial baker's yeast. Another non-Saccharomyces cerevisiae species, identified as the pentose utilizing Pichia galeiformis, was also recovered in the last tanks of the process where the hexose to pentose sugar ratio and the inhibitory pressure are expected to be the lowest.

No MeSH data available.


Related in: MedlinePlus