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Diagnostic importance of AgNOR pleomorphism in cervical carcinogenesis.

Srivastava A, Srivastava S, Bansal C, Misra J - Ecancermedicalscience (2013)

Bottom Line: Nucleolar organizer region (NOR) associated proteins are argyrophilic and visualized by silver stains.Silver nitrate staining was performed in 50 cervical smears each of cytologically diagnosed normal, inflammatory, low grade squamous intraepithelial lesion (LSIL), High-grade squamous intraepithelial lesion (HSIL), and squamous cell carcinoma cases registered at C.S.M.Medical University, Lucknow, India.

View Article: PubMed Central - PubMed

Affiliation: Pathology Department, Era's Medical College and Hospital, Lucknow, India.

ABSTRACT

Objective: Nucleolar organizer region (NOR) associated proteins are argyrophilic and visualized by silver stains. AgNOR pleomorphic dots increase in cancer and most researchers have done a common count of single dots. Pleomorphic dots are few and perhaps indicate a more severe prognosis. The present study was aimed at investigating the relative preponderance and diagnostic value of both pleomorphic and single AgNOR dots in cervical carcinogenesis.

Study design: Silver nitrate staining was performed in 50 cervical smears each of cytologically diagnosed normal, inflammatory, low grade squamous intraepithelial lesion (LSIL), High-grade squamous intraepithelial lesion (HSIL), and squamous cell carcinoma cases registered at C.S.M. Medical University, Lucknow, India.

Results: The accumulated data revealed a positive and significant correlation of cell counts of both pleomorphic (r = 0.94; p < 0.01) and single dots (r = 0.95; p < 0.01) with disease severity. The rate of increase in cell counts of pleomorphic dots (β = 2.61) was 1.1 times higher than the rate of increase in cell counts of single dots (β = 2.29).

Conclusion: This study indicates the diagnostic potential of pleomorphic dots in the process of cervical carcinogenesis. The number of pleomorphic dots also varies significantly in different types of SIL, which may help in discriminating precancerous lesions of the cervix.

No MeSH data available.


Related in: MedlinePlus

Squamous cell carcinoma cells dispersed in sheet exhibiting 8–10 pleomorphic dots (AgNOR × 100)
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figure4: Squamous cell carcinoma cells dispersed in sheet exhibiting 8–10 pleomorphic dots (AgNOR × 100)

Mentions: In normal smears, the number of total AgNOR dots ranged from 1 to 2, and in 41.8% of cases the dots were pleomorphic. In the inflammatory smears, the number of AgNOR dots varied from 2 to 3 (Figure 1), and the percentage of cases showing pleomorphic dots was 45.3%. In LSIL, total AgNOR dots were found to be 3–4 (Figure 2), and the percentage of cases showing pleomorphic dots was 48.3%. In the case of HSIL, AgNOR dots varied from 5 to 7 (Figure 3), and the percentage of cases showing pleomorphic dots was 56.7%. In the frank cancer cases, the total AgNOR dots were found to be 8–10 (Figure 4) and in 62.9% of cases, the dots were pleomorphic.


Diagnostic importance of AgNOR pleomorphism in cervical carcinogenesis.

Srivastava A, Srivastava S, Bansal C, Misra J - Ecancermedicalscience (2013)

Squamous cell carcinoma cells dispersed in sheet exhibiting 8–10 pleomorphic dots (AgNOR × 100)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3539855&req=5

figure4: Squamous cell carcinoma cells dispersed in sheet exhibiting 8–10 pleomorphic dots (AgNOR × 100)
Mentions: In normal smears, the number of total AgNOR dots ranged from 1 to 2, and in 41.8% of cases the dots were pleomorphic. In the inflammatory smears, the number of AgNOR dots varied from 2 to 3 (Figure 1), and the percentage of cases showing pleomorphic dots was 45.3%. In LSIL, total AgNOR dots were found to be 3–4 (Figure 2), and the percentage of cases showing pleomorphic dots was 48.3%. In the case of HSIL, AgNOR dots varied from 5 to 7 (Figure 3), and the percentage of cases showing pleomorphic dots was 56.7%. In the frank cancer cases, the total AgNOR dots were found to be 8–10 (Figure 4) and in 62.9% of cases, the dots were pleomorphic.

Bottom Line: Nucleolar organizer region (NOR) associated proteins are argyrophilic and visualized by silver stains.Silver nitrate staining was performed in 50 cervical smears each of cytologically diagnosed normal, inflammatory, low grade squamous intraepithelial lesion (LSIL), High-grade squamous intraepithelial lesion (HSIL), and squamous cell carcinoma cases registered at C.S.M.Medical University, Lucknow, India.

View Article: PubMed Central - PubMed

Affiliation: Pathology Department, Era's Medical College and Hospital, Lucknow, India.

ABSTRACT

Objective: Nucleolar organizer region (NOR) associated proteins are argyrophilic and visualized by silver stains. AgNOR pleomorphic dots increase in cancer and most researchers have done a common count of single dots. Pleomorphic dots are few and perhaps indicate a more severe prognosis. The present study was aimed at investigating the relative preponderance and diagnostic value of both pleomorphic and single AgNOR dots in cervical carcinogenesis.

Study design: Silver nitrate staining was performed in 50 cervical smears each of cytologically diagnosed normal, inflammatory, low grade squamous intraepithelial lesion (LSIL), High-grade squamous intraepithelial lesion (HSIL), and squamous cell carcinoma cases registered at C.S.M. Medical University, Lucknow, India.

Results: The accumulated data revealed a positive and significant correlation of cell counts of both pleomorphic (r = 0.94; p < 0.01) and single dots (r = 0.95; p < 0.01) with disease severity. The rate of increase in cell counts of pleomorphic dots (β = 2.61) was 1.1 times higher than the rate of increase in cell counts of single dots (β = 2.29).

Conclusion: This study indicates the diagnostic potential of pleomorphic dots in the process of cervical carcinogenesis. The number of pleomorphic dots also varies significantly in different types of SIL, which may help in discriminating precancerous lesions of the cervix.

No MeSH data available.


Related in: MedlinePlus