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Evidence of nNOS and ChAT positive phenotypes in nervous ganglia of the retrostyloid space.

Andrei F, Didilescu AC, Rusu MC - J Med Life (2012)

Bottom Line: S100 protein distinctively labelled the satellite glial cells ensheating the respective neurons.To evaluate a functional role of these phenotypes in ontogenesis, the specific anatomic circuits should be further checked.Differences in immune labelling should be evaluated by use of similar antibodies from different manufacturers.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Faculty of Medicine, Victor Babeş University of Medicine and Pharmacy, Timişoara, Romania.

ABSTRACT
The cholinergic and nitrergic phenotypes in human fetal ganglia (inferior) of the glossopharyngeal and vagus nerves were overlooked in basic research. Lack of a positive neuronal NO synthase (nNOS) phenotype in the inferior vagal fetal ganglion was recently suggested to be an individually variable phenotype. Choline acetyltransferase (ChAT) was not evaluated previously in ontogenesis. We aimed to evaluate these phenotypes in human midterm fetuses. Samples from five specimens with gestational ages varying from 4 to 6 months were used. Immunohistochemistry for nNOS, ChAT, neurofilaments, and S100 protein was performed. Neuronal somata were positively stained for nNOS, ChAT and neurofilaments in the inferior glossopharyngeal and vagal ganglia. S100 protein distinctively labelled the satellite glial cells ensheating the respective neurons. In human midterm fetuses vagal and glossopharyngeal inferior ganglia are nitrergic and cholinergic. To evaluate a functional role of these phenotypes in ontogenesis, the specific anatomic circuits should be further checked. Differences in immune labelling should be evaluated by use of similar antibodies from different manufacturers.

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Transverse cuts of the retrostyloid space. Immune labelling for nNOS (A, B), ChAT (C) and S100 protein (D). 1. internal carotid artery; 2. glossopharyngeal nerve, inferior ganglion; 3. inferior vagal ganglion (also depicted in B, C and D).
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Figure 1: Transverse cuts of the retrostyloid space. Immune labelling for nNOS (A, B), ChAT (C) and S100 protein (D). 1. internal carotid artery; 2. glossopharyngeal nerve, inferior ganglion; 3. inferior vagal ganglion (also depicted in B, C and D).

Mentions: Both ganglia, the inferior vagal ganglion (nodose ganglion) and the inferior ganglion of the glossopharyngeal nerve (petrosal ganglion), presented immune positive reactions of the neuronal somata when labelled with antibodies against the neurofilaments triplet, nNOS and ChAT (Fig. 1A-C), in all samples. Usually, each neuron was completely surrounded by a capsule built-up by satellite glial cells which were S100 protein immune positive (Fig. 1D).


Evidence of nNOS and ChAT positive phenotypes in nervous ganglia of the retrostyloid space.

Andrei F, Didilescu AC, Rusu MC - J Med Life (2012)

Transverse cuts of the retrostyloid space. Immune labelling for nNOS (A, B), ChAT (C) and S100 protein (D). 1. internal carotid artery; 2. glossopharyngeal nerve, inferior ganglion; 3. inferior vagal ganglion (also depicted in B, C and D).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3539831&req=5

Figure 1: Transverse cuts of the retrostyloid space. Immune labelling for nNOS (A, B), ChAT (C) and S100 protein (D). 1. internal carotid artery; 2. glossopharyngeal nerve, inferior ganglion; 3. inferior vagal ganglion (also depicted in B, C and D).
Mentions: Both ganglia, the inferior vagal ganglion (nodose ganglion) and the inferior ganglion of the glossopharyngeal nerve (petrosal ganglion), presented immune positive reactions of the neuronal somata when labelled with antibodies against the neurofilaments triplet, nNOS and ChAT (Fig. 1A-C), in all samples. Usually, each neuron was completely surrounded by a capsule built-up by satellite glial cells which were S100 protein immune positive (Fig. 1D).

Bottom Line: S100 protein distinctively labelled the satellite glial cells ensheating the respective neurons.To evaluate a functional role of these phenotypes in ontogenesis, the specific anatomic circuits should be further checked.Differences in immune labelling should be evaluated by use of similar antibodies from different manufacturers.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Faculty of Medicine, Victor Babeş University of Medicine and Pharmacy, Timişoara, Romania.

ABSTRACT
The cholinergic and nitrergic phenotypes in human fetal ganglia (inferior) of the glossopharyngeal and vagus nerves were overlooked in basic research. Lack of a positive neuronal NO synthase (nNOS) phenotype in the inferior vagal fetal ganglion was recently suggested to be an individually variable phenotype. Choline acetyltransferase (ChAT) was not evaluated previously in ontogenesis. We aimed to evaluate these phenotypes in human midterm fetuses. Samples from five specimens with gestational ages varying from 4 to 6 months were used. Immunohistochemistry for nNOS, ChAT, neurofilaments, and S100 protein was performed. Neuronal somata were positively stained for nNOS, ChAT and neurofilaments in the inferior glossopharyngeal and vagal ganglia. S100 protein distinctively labelled the satellite glial cells ensheating the respective neurons. In human midterm fetuses vagal and glossopharyngeal inferior ganglia are nitrergic and cholinergic. To evaluate a functional role of these phenotypes in ontogenesis, the specific anatomic circuits should be further checked. Differences in immune labelling should be evaluated by use of similar antibodies from different manufacturers.

Show MeSH
Related in: MedlinePlus