A link between the accumulation of DNA damage and loss of multi-potency of human mesenchymal stromal cells.
Bottom Line: Human mesenchymal stromal cells (hMSCs) represent an attractive cell source for clinic applications.Unfortunately, this leads to a significant decrease in their stemness.To identify the mechanism behind loss of multi-potency, hMSCs were expanded until replicative senescence and the concomitant molecular changes were characterized at regular intervals.
Affiliation: Department of Tissue Regeneration, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, The Netherlands. firstname.lastname@example.orgShow MeSH
Related in: MedlinePlus
Mentions: In order to investigate the differentiation capacity with respect to expansion, hMSCs from two donors were expanded until cessation of growth. As expected, hMSCs presented a continuous decrease in proliferation rate with increased time in culture (Fig. 1A). After an initial difference, both donors exhibited a similar level of PD/day until around 60 days in culture. At this point, the cells from donor 36 stopped proliferating while the cells from donor 24 displayed a temporary recovery followed by a permanent growth arrest. Although late passage cells did not proliferate, they were able to be maintained viable in culture for at least 3 more months before the experiment was ended. No spontaneous immortalization of hMSCs was ever observed and no clonal expansion was observed during this period of in vitro expansion.
Affiliation: Department of Tissue Regeneration, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, The Netherlands. email@example.com