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Characterization of human cytomegalovirus UL145 and UL136 genes in low-passage clinical isolates from infected Chinese infants.

Wang B, Hu JJ, Yan CF, Su HH, Ding JC, Guo YY, Ye N, Zhang SQ, Zhang XZ, Zhou SF - Med. Sci. Monit. (2011)

Bottom Line: The mRNA expression of both UL145 and UL136 was confirmed by reverse transcription (RT-PCR) assays.Both UL145 and UL136 are highly conserved.UL145 may act as an intranuclear regulating factor by direct binding to DNA, while UL136 may be a membrane receptor involving signal transduction.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Guangdong Women and Children's Hospital, Guangzhou, China. gzwbo@yahoo.com.cn

ABSTRACT

Background: Human cytomegalovirus (HCMV) is a leading cause of morbidity and mortality in immunocompromised individuals. The unique long b' (ULB') region of HCMV contains at least 19 open reading frames (ORFs); however, little is known about the function of UL145 and UL136. We characterized UL145 and UL136 in low-passage clinical isolates from Chinese infants.

Material/methods: The clinical strains of HCMV were recovered from the urine from HCMV-infected infants. Human embryonic lung fibroblasts (HELFs) were infected with clinical isolates of HCMV, and the viral DNA and mRNA for UL145 and UL136 were analyzed by polymerase chain reaction (PCR) and sequencing techniques. We also predicted the structure and function of UL145 and UL136 proteins.

Results: Sixty-two Chinese infants infected with HCMV were recruited into this study and the clinical isolates were recovered from the urine. Two strains among the low-passage isolates, D2 and D3, were obtained. The UL145 and UL136 sequences were deposited with GenBank under accession numbers of DQ180367, DQ180381, DQ180377, and DQ180389. The mRNA expression of both UL145 and UL136 was confirmed by reverse transcription (RT-PCR) assays. UL145 was predicted to contain 1 protein kinase C phosphorylation site, 2 casein kinase II phosphorylation sites and a zinc finger structure. UL136 was predicted to contain a protein kinase C phosphorylation site, N-myristoylation site, cAMP- and cGMP-dependent protein kinase phosphorylation site and tyrosine kinase II phosphorylation site. Both UL145 and UL136 are highly conserved.

Conclusions: UL145 may act as an intranuclear regulating factor by direct binding to DNA, while UL136 may be a membrane receptor involving signal transduction.

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Related in: MedlinePlus

The cladogram of UL136 sequences from various HCMV strains.
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f6-medscimonit-17-8-cr423: The cladogram of UL136 sequences from various HCMV strains.

Mentions: We studied the genetic evolution and sequence variations of UL136 when the UL136 gene of Toledo was employed as the root using the MEGA 4 program (http://www.megasoftware.net/). A phylogenetic tree was constructed using the nucleotide sequence of the UL136 gene from the D2 isolates and 11 of those previously published in GenBank. The cladogram is shown in Figure 6. Three groups (clades) were found from the tree, but HCMV strains did not cluster preferentially based on the measure of divergence. A further analysis of the phylogenetic tree of UL145 and UL136 did not show any preferential clustering of clinical isolates (data not shown). The assessment of the genetic distance in HCMV strains in different groups of patients revealed an even distribution of viral sequences.


Characterization of human cytomegalovirus UL145 and UL136 genes in low-passage clinical isolates from infected Chinese infants.

Wang B, Hu JJ, Yan CF, Su HH, Ding JC, Guo YY, Ye N, Zhang SQ, Zhang XZ, Zhou SF - Med. Sci. Monit. (2011)

The cladogram of UL136 sequences from various HCMV strains.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539624&req=5

f6-medscimonit-17-8-cr423: The cladogram of UL136 sequences from various HCMV strains.
Mentions: We studied the genetic evolution and sequence variations of UL136 when the UL136 gene of Toledo was employed as the root using the MEGA 4 program (http://www.megasoftware.net/). A phylogenetic tree was constructed using the nucleotide sequence of the UL136 gene from the D2 isolates and 11 of those previously published in GenBank. The cladogram is shown in Figure 6. Three groups (clades) were found from the tree, but HCMV strains did not cluster preferentially based on the measure of divergence. A further analysis of the phylogenetic tree of UL145 and UL136 did not show any preferential clustering of clinical isolates (data not shown). The assessment of the genetic distance in HCMV strains in different groups of patients revealed an even distribution of viral sequences.

Bottom Line: The mRNA expression of both UL145 and UL136 was confirmed by reverse transcription (RT-PCR) assays.Both UL145 and UL136 are highly conserved.UL145 may act as an intranuclear regulating factor by direct binding to DNA, while UL136 may be a membrane receptor involving signal transduction.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Guangdong Women and Children's Hospital, Guangzhou, China. gzwbo@yahoo.com.cn

ABSTRACT

Background: Human cytomegalovirus (HCMV) is a leading cause of morbidity and mortality in immunocompromised individuals. The unique long b' (ULB') region of HCMV contains at least 19 open reading frames (ORFs); however, little is known about the function of UL145 and UL136. We characterized UL145 and UL136 in low-passage clinical isolates from Chinese infants.

Material/methods: The clinical strains of HCMV were recovered from the urine from HCMV-infected infants. Human embryonic lung fibroblasts (HELFs) were infected with clinical isolates of HCMV, and the viral DNA and mRNA for UL145 and UL136 were analyzed by polymerase chain reaction (PCR) and sequencing techniques. We also predicted the structure and function of UL145 and UL136 proteins.

Results: Sixty-two Chinese infants infected with HCMV were recruited into this study and the clinical isolates were recovered from the urine. Two strains among the low-passage isolates, D2 and D3, were obtained. The UL145 and UL136 sequences were deposited with GenBank under accession numbers of DQ180367, DQ180381, DQ180377, and DQ180389. The mRNA expression of both UL145 and UL136 was confirmed by reverse transcription (RT-PCR) assays. UL145 was predicted to contain 1 protein kinase C phosphorylation site, 2 casein kinase II phosphorylation sites and a zinc finger structure. UL136 was predicted to contain a protein kinase C phosphorylation site, N-myristoylation site, cAMP- and cGMP-dependent protein kinase phosphorylation site and tyrosine kinase II phosphorylation site. Both UL145 and UL136 are highly conserved.

Conclusions: UL145 may act as an intranuclear regulating factor by direct binding to DNA, while UL136 may be a membrane receptor involving signal transduction.

Show MeSH
Related in: MedlinePlus