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Characterization of human cytomegalovirus UL145 and UL136 genes in low-passage clinical isolates from infected Chinese infants.

Wang B, Hu JJ, Yan CF, Su HH, Ding JC, Guo YY, Ye N, Zhang SQ, Zhang XZ, Zhou SF - Med. Sci. Monit. (2011)

Bottom Line: The mRNA expression of both UL145 and UL136 was confirmed by reverse transcription (RT-PCR) assays.Both UL145 and UL136 are highly conserved.UL145 may act as an intranuclear regulating factor by direct binding to DNA, while UL136 may be a membrane receptor involving signal transduction.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Guangdong Women and Children's Hospital, Guangzhou, China. gzwbo@yahoo.com.cn

ABSTRACT

Background: Human cytomegalovirus (HCMV) is a leading cause of morbidity and mortality in immunocompromised individuals. The unique long b' (ULB') region of HCMV contains at least 19 open reading frames (ORFs); however, little is known about the function of UL145 and UL136. We characterized UL145 and UL136 in low-passage clinical isolates from Chinese infants.

Material/methods: The clinical strains of HCMV were recovered from the urine from HCMV-infected infants. Human embryonic lung fibroblasts (HELFs) were infected with clinical isolates of HCMV, and the viral DNA and mRNA for UL145 and UL136 were analyzed by polymerase chain reaction (PCR) and sequencing techniques. We also predicted the structure and function of UL145 and UL136 proteins.

Results: Sixty-two Chinese infants infected with HCMV were recruited into this study and the clinical isolates were recovered from the urine. Two strains among the low-passage isolates, D2 and D3, were obtained. The UL145 and UL136 sequences were deposited with GenBank under accession numbers of DQ180367, DQ180381, DQ180377, and DQ180389. The mRNA expression of both UL145 and UL136 was confirmed by reverse transcription (RT-PCR) assays. UL145 was predicted to contain 1 protein kinase C phosphorylation site, 2 casein kinase II phosphorylation sites and a zinc finger structure. UL136 was predicted to contain a protein kinase C phosphorylation site, N-myristoylation site, cAMP- and cGMP-dependent protein kinase phosphorylation site and tyrosine kinase II phosphorylation site. Both UL145 and UL136 are highly conserved.

Conclusions: UL145 may act as an intranuclear regulating factor by direct binding to DNA, while UL136 may be a membrane receptor involving signal transduction.

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Related in: MedlinePlus

Comparison of amino acid sequences encoded by HCMV UL145 (A) and UL136 (B) genes. The deducted amino acid number was 131 and 241 for UL145 and UL136, respectively.
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f5-medscimonit-17-8-cr423: Comparison of amino acid sequences encoded by HCMV UL145 (A) and UL136 (B) genes. The deducted amino acid number was 131 and 241 for UL145 and UL136, respectively.

Mentions: The UL136 gene cloned from the D2 isolates was 723-bp long (GenBank accession No.: DQ180377). UL136 was deducted to encode a protein of 240 amino acids. Compared to other reference strains, the sequence of UL136 was highly conserved, with aberration rates of 1.8–2.9% (Figure 5). A total of 30 out of 1019 nucleotides in UL136 from the D2 isolates showed variations. In terms of amino acid sequence, the protein encoded by the UL136 gene from the D2 isolates had 14 variations and the mutation rate was 1.6–3.7% compared to other strains.


Characterization of human cytomegalovirus UL145 and UL136 genes in low-passage clinical isolates from infected Chinese infants.

Wang B, Hu JJ, Yan CF, Su HH, Ding JC, Guo YY, Ye N, Zhang SQ, Zhang XZ, Zhou SF - Med. Sci. Monit. (2011)

Comparison of amino acid sequences encoded by HCMV UL145 (A) and UL136 (B) genes. The deducted amino acid number was 131 and 241 for UL145 and UL136, respectively.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539624&req=5

f5-medscimonit-17-8-cr423: Comparison of amino acid sequences encoded by HCMV UL145 (A) and UL136 (B) genes. The deducted amino acid number was 131 and 241 for UL145 and UL136, respectively.
Mentions: The UL136 gene cloned from the D2 isolates was 723-bp long (GenBank accession No.: DQ180377). UL136 was deducted to encode a protein of 240 amino acids. Compared to other reference strains, the sequence of UL136 was highly conserved, with aberration rates of 1.8–2.9% (Figure 5). A total of 30 out of 1019 nucleotides in UL136 from the D2 isolates showed variations. In terms of amino acid sequence, the protein encoded by the UL136 gene from the D2 isolates had 14 variations and the mutation rate was 1.6–3.7% compared to other strains.

Bottom Line: The mRNA expression of both UL145 and UL136 was confirmed by reverse transcription (RT-PCR) assays.Both UL145 and UL136 are highly conserved.UL145 may act as an intranuclear regulating factor by direct binding to DNA, while UL136 may be a membrane receptor involving signal transduction.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Guangdong Women and Children's Hospital, Guangzhou, China. gzwbo@yahoo.com.cn

ABSTRACT

Background: Human cytomegalovirus (HCMV) is a leading cause of morbidity and mortality in immunocompromised individuals. The unique long b' (ULB') region of HCMV contains at least 19 open reading frames (ORFs); however, little is known about the function of UL145 and UL136. We characterized UL145 and UL136 in low-passage clinical isolates from Chinese infants.

Material/methods: The clinical strains of HCMV were recovered from the urine from HCMV-infected infants. Human embryonic lung fibroblasts (HELFs) were infected with clinical isolates of HCMV, and the viral DNA and mRNA for UL145 and UL136 were analyzed by polymerase chain reaction (PCR) and sequencing techniques. We also predicted the structure and function of UL145 and UL136 proteins.

Results: Sixty-two Chinese infants infected with HCMV were recruited into this study and the clinical isolates were recovered from the urine. Two strains among the low-passage isolates, D2 and D3, were obtained. The UL145 and UL136 sequences were deposited with GenBank under accession numbers of DQ180367, DQ180381, DQ180377, and DQ180389. The mRNA expression of both UL145 and UL136 was confirmed by reverse transcription (RT-PCR) assays. UL145 was predicted to contain 1 protein kinase C phosphorylation site, 2 casein kinase II phosphorylation sites and a zinc finger structure. UL136 was predicted to contain a protein kinase C phosphorylation site, N-myristoylation site, cAMP- and cGMP-dependent protein kinase phosphorylation site and tyrosine kinase II phosphorylation site. Both UL145 and UL136 are highly conserved.

Conclusions: UL145 may act as an intranuclear regulating factor by direct binding to DNA, while UL136 may be a membrane receptor involving signal transduction.

Show MeSH
Related in: MedlinePlus