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The effects of in vivo and ex vivo various degrees of cold exposure on erythrocyte deformability and aggregation.

Erken G, Erken HA, Bor-Kucukatay M, Kucukatay V, Genc O - Med. Sci. Monit. (2011)

Bottom Line: The in vivo group was further divided into control (AR), AC (4°C, 2 hours) and ALTC (4°C, 6 hours) subgroups; and the ex vivo group was divided into control (BR) and BC (4°C, 2 hours) subgroups.Aggregation of ex vivo groups was lower compared to in vivo groups.Cold exposure at various temperatures did not cause alterations in plasma total oxidant antioxidant status and oxidative stress index (TOS, TAS, OSI) when considered together.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Faculty of Medicine, Pamukkale University, Kinikli, Denizli, Turkey. gulemmun@pau.edu.tr

ABSTRACT

Background: This study aimed to investigate alterations in hemorheology by cold exposure, in vivo and ex vivo, and to determine their relationship to oxidative stress.

Material/methods: Rats were divided into 2 in vivo and ex vivo cold exposure groups. The in vivo group was further divided into control (AR), AC (4°C, 2 hours) and ALTC (4°C, 6 hours) subgroups; and the ex vivo group was divided into control (BR) and BC (4°C, 2 hours) subgroups. Blood samples were used for the determination of erythrocyte deformability, aggregation, and oxidative stress parameters.

Results: Erythrocyte deformability and aggregation were not affected by 2-hour ex vivo cold exposure. While 2 hour in vivo cold exposure reduced erythrocyte deformability, it returned to normal after 6 hours, possibly due the compensation by acute neuroendocrine response. Six hours of cold exposure decreased aggregation index, and might be an adaptive mechanism allowing the continuation of circulation. Aggregation of ex vivo groups was lower compared to in vivo groups. Cold exposure at various temperatures did not cause alterations in plasma total oxidant antioxidant status and oxidative stress index (TOS, TAS, OSI) when considered together.

Conclusions: Results of this study indicate that the alterations observed in hemorheological parameters due to cold exposure are far from being explained by the oxidative stress parameters determined herein.

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Related in: MedlinePlus

The total antioxidant status of all groups. AR: Animal at room air, AC: Animal in cold, ALTC: Animal in long term cold, BR: Blood at room air, BC: Blood in cold. Values are expressed as means ±SE. * p<0.05, difference from BC and ALTC groups.
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f6-medscimonit-17-8-br209: The total antioxidant status of all groups. AR: Animal at room air, AC: Animal in cold, ALTC: Animal in long term cold, BR: Blood at room air, BC: Blood in cold. Values are expressed as means ±SE. * p<0.05, difference from BC and ALTC groups.

Mentions: The parameters showing the oxidant (TOS) and antioxidant status (TAS), as well as the oxidative stress index of the groups, are presented in Figures 5, 6 and 7, respectively. Total oxidant status (TOS) of the blood at room air (BR) and blood in cold (BC) groups were decreased compared to animal in cold (AC, p<0.01) and animal in long-term cold (ALTC, p<0.001) groups. TOS of the animal in long-term cold (ALTC) group was highest of the groups (Figure 5). On the other hand, total antioxidant status (TAS) of the blood at room air group was lower compared to blood in cold (BC) and animal in long-term cold (ALTC) groups (p<0.05). The oxidative stress index (OSI), which was calculated for each animal as: TOS/TAS, although not statistically significant, was found to be higher for the animal in cold (AC) and animal in long-term cold (ALTC) groups.


The effects of in vivo and ex vivo various degrees of cold exposure on erythrocyte deformability and aggregation.

Erken G, Erken HA, Bor-Kucukatay M, Kucukatay V, Genc O - Med. Sci. Monit. (2011)

The total antioxidant status of all groups. AR: Animal at room air, AC: Animal in cold, ALTC: Animal in long term cold, BR: Blood at room air, BC: Blood in cold. Values are expressed as means ±SE. * p<0.05, difference from BC and ALTC groups.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539619&req=5

f6-medscimonit-17-8-br209: The total antioxidant status of all groups. AR: Animal at room air, AC: Animal in cold, ALTC: Animal in long term cold, BR: Blood at room air, BC: Blood in cold. Values are expressed as means ±SE. * p<0.05, difference from BC and ALTC groups.
Mentions: The parameters showing the oxidant (TOS) and antioxidant status (TAS), as well as the oxidative stress index of the groups, are presented in Figures 5, 6 and 7, respectively. Total oxidant status (TOS) of the blood at room air (BR) and blood in cold (BC) groups were decreased compared to animal in cold (AC, p<0.01) and animal in long-term cold (ALTC, p<0.001) groups. TOS of the animal in long-term cold (ALTC) group was highest of the groups (Figure 5). On the other hand, total antioxidant status (TAS) of the blood at room air group was lower compared to blood in cold (BC) and animal in long-term cold (ALTC) groups (p<0.05). The oxidative stress index (OSI), which was calculated for each animal as: TOS/TAS, although not statistically significant, was found to be higher for the animal in cold (AC) and animal in long-term cold (ALTC) groups.

Bottom Line: The in vivo group was further divided into control (AR), AC (4°C, 2 hours) and ALTC (4°C, 6 hours) subgroups; and the ex vivo group was divided into control (BR) and BC (4°C, 2 hours) subgroups.Aggregation of ex vivo groups was lower compared to in vivo groups.Cold exposure at various temperatures did not cause alterations in plasma total oxidant antioxidant status and oxidative stress index (TOS, TAS, OSI) when considered together.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Faculty of Medicine, Pamukkale University, Kinikli, Denizli, Turkey. gulemmun@pau.edu.tr

ABSTRACT

Background: This study aimed to investigate alterations in hemorheology by cold exposure, in vivo and ex vivo, and to determine their relationship to oxidative stress.

Material/methods: Rats were divided into 2 in vivo and ex vivo cold exposure groups. The in vivo group was further divided into control (AR), AC (4°C, 2 hours) and ALTC (4°C, 6 hours) subgroups; and the ex vivo group was divided into control (BR) and BC (4°C, 2 hours) subgroups. Blood samples were used for the determination of erythrocyte deformability, aggregation, and oxidative stress parameters.

Results: Erythrocyte deformability and aggregation were not affected by 2-hour ex vivo cold exposure. While 2 hour in vivo cold exposure reduced erythrocyte deformability, it returned to normal after 6 hours, possibly due the compensation by acute neuroendocrine response. Six hours of cold exposure decreased aggregation index, and might be an adaptive mechanism allowing the continuation of circulation. Aggregation of ex vivo groups was lower compared to in vivo groups. Cold exposure at various temperatures did not cause alterations in plasma total oxidant antioxidant status and oxidative stress index (TOS, TAS, OSI) when considered together.

Conclusions: Results of this study indicate that the alterations observed in hemorheological parameters due to cold exposure are far from being explained by the oxidative stress parameters determined herein.

Show MeSH
Related in: MedlinePlus