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The effects of in vivo and ex vivo various degrees of cold exposure on erythrocyte deformability and aggregation.

Erken G, Erken HA, Bor-Kucukatay M, Kucukatay V, Genc O - Med. Sci. Monit. (2011)

Bottom Line: The in vivo group was further divided into control (AR), AC (4°C, 2 hours) and ALTC (4°C, 6 hours) subgroups; and the ex vivo group was divided into control (BR) and BC (4°C, 2 hours) subgroups.Aggregation of ex vivo groups was lower compared to in vivo groups.Cold exposure at various temperatures did not cause alterations in plasma total oxidant antioxidant status and oxidative stress index (TOS, TAS, OSI) when considered together.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Faculty of Medicine, Pamukkale University, Kinikli, Denizli, Turkey. gulemmun@pau.edu.tr

ABSTRACT

Background: This study aimed to investigate alterations in hemorheology by cold exposure, in vivo and ex vivo, and to determine their relationship to oxidative stress.

Material/methods: Rats were divided into 2 in vivo and ex vivo cold exposure groups. The in vivo group was further divided into control (AR), AC (4°C, 2 hours) and ALTC (4°C, 6 hours) subgroups; and the ex vivo group was divided into control (BR) and BC (4°C, 2 hours) subgroups. Blood samples were used for the determination of erythrocyte deformability, aggregation, and oxidative stress parameters.

Results: Erythrocyte deformability and aggregation were not affected by 2-hour ex vivo cold exposure. While 2 hour in vivo cold exposure reduced erythrocyte deformability, it returned to normal after 6 hours, possibly due the compensation by acute neuroendocrine response. Six hours of cold exposure decreased aggregation index, and might be an adaptive mechanism allowing the continuation of circulation. Aggregation of ex vivo groups was lower compared to in vivo groups. Cold exposure at various temperatures did not cause alterations in plasma total oxidant antioxidant status and oxidative stress index (TOS, TAS, OSI) when considered together.

Conclusions: Results of this study indicate that the alterations observed in hemorheological parameters due to cold exposure are far from being explained by the oxidative stress parameters determined herein.

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Related in: MedlinePlus

Red blood cell (RBC) elongation index (EI) values of all groups mesured at 1.69 Pascal (Pa) shear stress. AR: Animal at room air, AC: Animal in cold, ALTC: Animal in long term cold, BR: Blood at room air, BC: Blood in cold. Values are expressed as means ±SE. * p<0.05, difference from AR and ALTC groups; # p<0.01, difference from BR and BC groups.
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f1-medscimonit-17-8-br209: Red blood cell (RBC) elongation index (EI) values of all groups mesured at 1.69 Pascal (Pa) shear stress. AR: Animal at room air, AC: Animal in cold, ALTC: Animal in long term cold, BR: Blood at room air, BC: Blood in cold. Values are expressed as means ±SE. * p<0.05, difference from AR and ALTC groups; # p<0.01, difference from BR and BC groups.

Mentions: RBC deformability (assessed as the elongation index EI) for the RBCs of all experimental groups was measured at 9 shear stresses between 0.3 and 30 Pa, and EI values measured at 1.69 Pa (Figure 1). RBC deformability of animals in the cold (AC) group was found to be lower compared to the other groups p<0.05 compared to animals at room air (AR) and animal in long-term cold (ALTC) groups and p<0.01 compared to blood at room air (BR) and blood in cold (BC) groups.


The effects of in vivo and ex vivo various degrees of cold exposure on erythrocyte deformability and aggregation.

Erken G, Erken HA, Bor-Kucukatay M, Kucukatay V, Genc O - Med. Sci. Monit. (2011)

Red blood cell (RBC) elongation index (EI) values of all groups mesured at 1.69 Pascal (Pa) shear stress. AR: Animal at room air, AC: Animal in cold, ALTC: Animal in long term cold, BR: Blood at room air, BC: Blood in cold. Values are expressed as means ±SE. * p<0.05, difference from AR and ALTC groups; # p<0.01, difference from BR and BC groups.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539619&req=5

f1-medscimonit-17-8-br209: Red blood cell (RBC) elongation index (EI) values of all groups mesured at 1.69 Pascal (Pa) shear stress. AR: Animal at room air, AC: Animal in cold, ALTC: Animal in long term cold, BR: Blood at room air, BC: Blood in cold. Values are expressed as means ±SE. * p<0.05, difference from AR and ALTC groups; # p<0.01, difference from BR and BC groups.
Mentions: RBC deformability (assessed as the elongation index EI) for the RBCs of all experimental groups was measured at 9 shear stresses between 0.3 and 30 Pa, and EI values measured at 1.69 Pa (Figure 1). RBC deformability of animals in the cold (AC) group was found to be lower compared to the other groups p<0.05 compared to animals at room air (AR) and animal in long-term cold (ALTC) groups and p<0.01 compared to blood at room air (BR) and blood in cold (BC) groups.

Bottom Line: The in vivo group was further divided into control (AR), AC (4°C, 2 hours) and ALTC (4°C, 6 hours) subgroups; and the ex vivo group was divided into control (BR) and BC (4°C, 2 hours) subgroups.Aggregation of ex vivo groups was lower compared to in vivo groups.Cold exposure at various temperatures did not cause alterations in plasma total oxidant antioxidant status and oxidative stress index (TOS, TAS, OSI) when considered together.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Faculty of Medicine, Pamukkale University, Kinikli, Denizli, Turkey. gulemmun@pau.edu.tr

ABSTRACT

Background: This study aimed to investigate alterations in hemorheology by cold exposure, in vivo and ex vivo, and to determine their relationship to oxidative stress.

Material/methods: Rats were divided into 2 in vivo and ex vivo cold exposure groups. The in vivo group was further divided into control (AR), AC (4°C, 2 hours) and ALTC (4°C, 6 hours) subgroups; and the ex vivo group was divided into control (BR) and BC (4°C, 2 hours) subgroups. Blood samples were used for the determination of erythrocyte deformability, aggregation, and oxidative stress parameters.

Results: Erythrocyte deformability and aggregation were not affected by 2-hour ex vivo cold exposure. While 2 hour in vivo cold exposure reduced erythrocyte deformability, it returned to normal after 6 hours, possibly due the compensation by acute neuroendocrine response. Six hours of cold exposure decreased aggregation index, and might be an adaptive mechanism allowing the continuation of circulation. Aggregation of ex vivo groups was lower compared to in vivo groups. Cold exposure at various temperatures did not cause alterations in plasma total oxidant antioxidant status and oxidative stress index (TOS, TAS, OSI) when considered together.

Conclusions: Results of this study indicate that the alterations observed in hemorheological parameters due to cold exposure are far from being explained by the oxidative stress parameters determined herein.

Show MeSH
Related in: MedlinePlus