Limits...
Lymphotropic herpesvirus DNA detection in patients with active CMV infection - a possible role in the course of CMV infection after hematopoietic stem cell transplantation.

Zawilinska B, Kopec J, Szostek S, Piatkowska-Jakubas B, Skotnicki AB, Kosz-Vnenchak M - Med. Sci. Monit. (2011)

Bottom Line: In Group I, the mean CMV load was significantly higher than in Group II, and the clinical condition of Group I patients was poorer.In addition, CMV presence was often preceded by another herpesvirus.The results suggest that other herpesviruses, mainly HHV-7, could predispose CMV to cause chronic infection.

View Article: PubMed Central - PubMed

Affiliation: Department of Virology, Chair of Microbiology, Jagiellonian University Medical College, Cracow, Poland. mbzawili@cm-uj.krakow.pl

ABSTRACT

Background: The natural history of cytomegalovirus (CMV) infection and disease in transplant recipients prompts researchers to look for other factors contributing to this infection. The ubiquity of lymphotropic herpesviruses (EBV, HHV-6, and HHV-7) and the possibility of their activation during immunosuppression may suggest their participation in progression of CMV infection in patients after hematopoietic stem cell transplantation (HSCT).

Material/methods: The presence of CMV, EBV, HHV-6 and HHV-7 was confirmed through detection of viral DNA isolated from leukocytes. Allo-HSCT recipients (n=55) were examined repeatedly within the average period of 14±7.3 months post-transplant.

Results: CMV DNA was detected in 24% of samples, while EBV, HHV-6 and HHV-7 were detected in 20%, 15% and 14% of samples, respectively. Based on the presence of CMV infection at particular time-points (months) after transplantation, the recipients were divided into 3 groups: Group I (N=15) with persistent infection, Group II (N=20) with transient infection, and Group III (N=20) without CMV infection. In Group I, the mean CMV load was significantly higher than in Group II, and the clinical condition of Group I patients was poorer. All these patients manifested clinical symptoms, and all had episodes of GvHD. All Group I patients developed multiple infections; EBV in 80%, HHV-6 in 47% and HHV-7 in 87% of patients. In the remaining groups, with the exception of HHV-6 in group II, the frequency of infected patients was lower. In addition, CMV presence was often preceded by another herpesvirus.

Conclusions: The results suggest that other herpesviruses, mainly HHV-7, could predispose CMV to cause chronic infection.

Show MeSH

Related in: MedlinePlus

Relative frequency of CMV, EBV, HHV-6 and HHV-7 DNA in peripheral blood leukocytes post-transplant.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3539614&req=5

f2-medscimonit-17-8-cr432: Relative frequency of CMV, EBV, HHV-6 and HHV-7 DNA in peripheral blood leukocytes post-transplant.

Mentions: The appearance of particular viruses following transplantation is shown in Figure 2, in which the relative frequency of viruses adjusted to the same number of samples examined for each virus was used. This approach was necessary because in the first months after transplantation, the patients were monitored more frequently as compared to the later period. Detection of CMV predominated in the early months after allo-HSCT; however, in late period CMV also appeared in both Group I and Group II. The statistical analysis of differences in CMV DNA detection in consecutive months after transplantation was significant in Group I and Group II (Wilcoxon’s test, p=0.0023). A similar relationship in Group I and Group II was observed for HHV-7 (Wilcoxon’s test, p=0.0052), and also for Group I and Group III (Wilcoxon’s test, p=0.0131). The kinetics of HHV-6 appearance was significantly different between Group II and Group III (Wilcoxon’s test, p=0.0159).


Lymphotropic herpesvirus DNA detection in patients with active CMV infection - a possible role in the course of CMV infection after hematopoietic stem cell transplantation.

Zawilinska B, Kopec J, Szostek S, Piatkowska-Jakubas B, Skotnicki AB, Kosz-Vnenchak M - Med. Sci. Monit. (2011)

Relative frequency of CMV, EBV, HHV-6 and HHV-7 DNA in peripheral blood leukocytes post-transplant.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539614&req=5

f2-medscimonit-17-8-cr432: Relative frequency of CMV, EBV, HHV-6 and HHV-7 DNA in peripheral blood leukocytes post-transplant.
Mentions: The appearance of particular viruses following transplantation is shown in Figure 2, in which the relative frequency of viruses adjusted to the same number of samples examined for each virus was used. This approach was necessary because in the first months after transplantation, the patients were monitored more frequently as compared to the later period. Detection of CMV predominated in the early months after allo-HSCT; however, in late period CMV also appeared in both Group I and Group II. The statistical analysis of differences in CMV DNA detection in consecutive months after transplantation was significant in Group I and Group II (Wilcoxon’s test, p=0.0023). A similar relationship in Group I and Group II was observed for HHV-7 (Wilcoxon’s test, p=0.0052), and also for Group I and Group III (Wilcoxon’s test, p=0.0131). The kinetics of HHV-6 appearance was significantly different between Group II and Group III (Wilcoxon’s test, p=0.0159).

Bottom Line: In Group I, the mean CMV load was significantly higher than in Group II, and the clinical condition of Group I patients was poorer.In addition, CMV presence was often preceded by another herpesvirus.The results suggest that other herpesviruses, mainly HHV-7, could predispose CMV to cause chronic infection.

View Article: PubMed Central - PubMed

Affiliation: Department of Virology, Chair of Microbiology, Jagiellonian University Medical College, Cracow, Poland. mbzawili@cm-uj.krakow.pl

ABSTRACT

Background: The natural history of cytomegalovirus (CMV) infection and disease in transplant recipients prompts researchers to look for other factors contributing to this infection. The ubiquity of lymphotropic herpesviruses (EBV, HHV-6, and HHV-7) and the possibility of their activation during immunosuppression may suggest their participation in progression of CMV infection in patients after hematopoietic stem cell transplantation (HSCT).

Material/methods: The presence of CMV, EBV, HHV-6 and HHV-7 was confirmed through detection of viral DNA isolated from leukocytes. Allo-HSCT recipients (n=55) were examined repeatedly within the average period of 14±7.3 months post-transplant.

Results: CMV DNA was detected in 24% of samples, while EBV, HHV-6 and HHV-7 were detected in 20%, 15% and 14% of samples, respectively. Based on the presence of CMV infection at particular time-points (months) after transplantation, the recipients were divided into 3 groups: Group I (N=15) with persistent infection, Group II (N=20) with transient infection, and Group III (N=20) without CMV infection. In Group I, the mean CMV load was significantly higher than in Group II, and the clinical condition of Group I patients was poorer. All these patients manifested clinical symptoms, and all had episodes of GvHD. All Group I patients developed multiple infections; EBV in 80%, HHV-6 in 47% and HHV-7 in 87% of patients. In the remaining groups, with the exception of HHV-6 in group II, the frequency of infected patients was lower. In addition, CMV presence was often preceded by another herpesvirus.

Conclusions: The results suggest that other herpesviruses, mainly HHV-7, could predispose CMV to cause chronic infection.

Show MeSH
Related in: MedlinePlus