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The expression levels of stem cell markers importin13, c-kit, CD146, and telomerase are decreased in endometrial polyps.

Hu J, Yuan R - Med. Sci. Monit. (2011)

Bottom Line: We detected the protein expression levels of IPO13, telomerase, CD146, caspase-3, bcl-2 and bax in EPs using S-P (Streptavidin-Peroxidase) immunohistochemistry.Western blotting was performed to determine the levels of importin13 and bcl-2 proteins in EPs.The expression levels of IPO13, c-kit, telomerase, caspase3, and bax were lower in the EP tissue compared to normal endometrial tissue during the proliferation and secretion phases of the menstrual cycle (p<0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, 1st Affiliated Hospital, Chongqing Medical University, Chongqing, China.

ABSTRACT

Background: To investigate the expression levels of importin13 (IPO13), c-kit, CD146, telomerase, caspase-3, bcl-2 and bax in endometrial polyps (EPs).

Material/methods: We detected the mRNA expression levels of IPO13, c-kit, bcl-2 and bax in endometrial polyps (EPs) using real-time PCR. We detected the protein expression levels of IPO13, telomerase, CD146, caspase-3, bcl-2 and bax in EPs using S-P (Streptavidin-Peroxidase) immunohistochemistry. Western blotting was performed to determine the levels of importin13 and bcl-2 proteins in EPs.

Results: The expression levels of IPO13, c-kit, telomerase, caspase3, and bax were lower in the EP tissue compared to normal endometrial tissue during the proliferation and secretion phases of the menstrual cycle (p<0.05). The expression of CD146 was decreased in the EP tissue compared to the normal endometrial tissue during the proliferation phase of the menstrual cycle (p<0.05). The expression of bcl-2 was increased in the EP tissue compared to the normal endometrial tissue during the proliferation and secretion phases of the menstrual cycle (p<0.05).

Conclusions: The expression levels of IPO13, c-kit, telomerase, caspase3, and bax were decreased; however, the expression of bcl-2 was increased in the EP tissue compared to the normal endometrial tissue. These findings suggest that the development of EPs is associated with the deregulated activities of the endometrial stem/progenitor cells and the decreased apoptosis of endometrial cells, with the latter being the major factor involved in the development of EPs.

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The expression levels of IPO13 and caspase3 in the endometrial tissues were detected by immunohistochemistry (400×). (A) Control endometrium at the proliferation phase. (B) Control endometrium at the secretion phase. (C) EP at the proliferation phase. (D) EP at the secretion phase. (E) Control endometrium at the proliferation phase. (F) Control endometrium at the secretion phase. (G) EP at the proliferation phase. (H) EP at the secretion phase. * p<0.05 (vs. control endometrium at the proliferation phase); ** p<0.05 (vs. control endometrium at the secretion phase); error bars, SEM.
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f2-medscimonit-17-8-br221: The expression levels of IPO13 and caspase3 in the endometrial tissues were detected by immunohistochemistry (400×). (A) Control endometrium at the proliferation phase. (B) Control endometrium at the secretion phase. (C) EP at the proliferation phase. (D) EP at the secretion phase. (E) Control endometrium at the proliferation phase. (F) Control endometrium at the secretion phase. (G) EP at the proliferation phase. (H) EP at the secretion phase. * p<0.05 (vs. control endometrium at the proliferation phase); ** p<0.05 (vs. control endometrium at the secretion phase); error bars, SEM.

Mentions: IPO13, telomerase, caspase3, bcl-2 and bax were expressed both in the cytoplasm and nucleus of the epithelial and stromal cells. CD146 was mainly expressed in the cytoplasm of the stromal vascular endothelial cells (Figure 1). Telomerase was strongly expressed in the endometrium during the proliferation or secretion phase in the control group; however, it was weakly expressed or undetectable in the EP patients. The telomerase staining seems to indicate that in the secretory phase diffuse glandular staining is decreased compared to the proliferative phase. CD146 was strongly expressed in the control group; however, it was weakly expressed in the EP patients (Figure 1). IPO13 was strongly expressed in the endometrium in the control group; however, it was weakly expressed or could not be detected in the EP patients (Figure 2). Caspase3 showed very strong expression in the endometrium during the proliferation phase and strong expression during the secretion phase in the control group; however, it was weakly expressed or could not be detected in the EP patients (Figure 2). Bax was strongly expressed in the control group; however, it was weakly expressed or could not be detected in the EP patients (Figure 3). Bcl-2 was weakly expressed in the control group; however, it was strongly expressed in the EP patients. Bcl2 staining was stronger in the stroma compared to the glands (Figure 3).


The expression levels of stem cell markers importin13, c-kit, CD146, and telomerase are decreased in endometrial polyps.

Hu J, Yuan R - Med. Sci. Monit. (2011)

The expression levels of IPO13 and caspase3 in the endometrial tissues were detected by immunohistochemistry (400×). (A) Control endometrium at the proliferation phase. (B) Control endometrium at the secretion phase. (C) EP at the proliferation phase. (D) EP at the secretion phase. (E) Control endometrium at the proliferation phase. (F) Control endometrium at the secretion phase. (G) EP at the proliferation phase. (H) EP at the secretion phase. * p<0.05 (vs. control endometrium at the proliferation phase); ** p<0.05 (vs. control endometrium at the secretion phase); error bars, SEM.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539613&req=5

f2-medscimonit-17-8-br221: The expression levels of IPO13 and caspase3 in the endometrial tissues were detected by immunohistochemistry (400×). (A) Control endometrium at the proliferation phase. (B) Control endometrium at the secretion phase. (C) EP at the proliferation phase. (D) EP at the secretion phase. (E) Control endometrium at the proliferation phase. (F) Control endometrium at the secretion phase. (G) EP at the proliferation phase. (H) EP at the secretion phase. * p<0.05 (vs. control endometrium at the proliferation phase); ** p<0.05 (vs. control endometrium at the secretion phase); error bars, SEM.
Mentions: IPO13, telomerase, caspase3, bcl-2 and bax were expressed both in the cytoplasm and nucleus of the epithelial and stromal cells. CD146 was mainly expressed in the cytoplasm of the stromal vascular endothelial cells (Figure 1). Telomerase was strongly expressed in the endometrium during the proliferation or secretion phase in the control group; however, it was weakly expressed or undetectable in the EP patients. The telomerase staining seems to indicate that in the secretory phase diffuse glandular staining is decreased compared to the proliferative phase. CD146 was strongly expressed in the control group; however, it was weakly expressed in the EP patients (Figure 1). IPO13 was strongly expressed in the endometrium in the control group; however, it was weakly expressed or could not be detected in the EP patients (Figure 2). Caspase3 showed very strong expression in the endometrium during the proliferation phase and strong expression during the secretion phase in the control group; however, it was weakly expressed or could not be detected in the EP patients (Figure 2). Bax was strongly expressed in the control group; however, it was weakly expressed or could not be detected in the EP patients (Figure 3). Bcl-2 was weakly expressed in the control group; however, it was strongly expressed in the EP patients. Bcl2 staining was stronger in the stroma compared to the glands (Figure 3).

Bottom Line: We detected the protein expression levels of IPO13, telomerase, CD146, caspase-3, bcl-2 and bax in EPs using S-P (Streptavidin-Peroxidase) immunohistochemistry.Western blotting was performed to determine the levels of importin13 and bcl-2 proteins in EPs.The expression levels of IPO13, c-kit, telomerase, caspase3, and bax were lower in the EP tissue compared to normal endometrial tissue during the proliferation and secretion phases of the menstrual cycle (p<0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, 1st Affiliated Hospital, Chongqing Medical University, Chongqing, China.

ABSTRACT

Background: To investigate the expression levels of importin13 (IPO13), c-kit, CD146, telomerase, caspase-3, bcl-2 and bax in endometrial polyps (EPs).

Material/methods: We detected the mRNA expression levels of IPO13, c-kit, bcl-2 and bax in endometrial polyps (EPs) using real-time PCR. We detected the protein expression levels of IPO13, telomerase, CD146, caspase-3, bcl-2 and bax in EPs using S-P (Streptavidin-Peroxidase) immunohistochemistry. Western blotting was performed to determine the levels of importin13 and bcl-2 proteins in EPs.

Results: The expression levels of IPO13, c-kit, telomerase, caspase3, and bax were lower in the EP tissue compared to normal endometrial tissue during the proliferation and secretion phases of the menstrual cycle (p<0.05). The expression of CD146 was decreased in the EP tissue compared to the normal endometrial tissue during the proliferation phase of the menstrual cycle (p<0.05). The expression of bcl-2 was increased in the EP tissue compared to the normal endometrial tissue during the proliferation and secretion phases of the menstrual cycle (p<0.05).

Conclusions: The expression levels of IPO13, c-kit, telomerase, caspase3, and bax were decreased; however, the expression of bcl-2 was increased in the EP tissue compared to the normal endometrial tissue. These findings suggest that the development of EPs is associated with the deregulated activities of the endometrial stem/progenitor cells and the decreased apoptosis of endometrial cells, with the latter being the major factor involved in the development of EPs.

Show MeSH
Related in: MedlinePlus